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In this study, silver nanoparticles (AgNPs) were green\u2010synthesized using <jats:styled-content style=\"fixed-case\"><jats:italic>Chromolaena odorata<\/jats:italic><\/jats:styled-content> (<jats:italic>CO<\/jats:italic>) and characterized using UV\u2013Vis spectroscopy, Fourier\u2010transform infrared spectroscopy (FTIR), scanning electron microscopy\/energy\u2010dispersive X\u2010ray spectroscopy (SEM\/EDS), x\u2010ray diffraction (XRD), thermogravimetric analysis (TGA), and transmission electron microscopy (TEM). The interactions of Co\u2010AgNPs, cisplatin, and their combination with biomolecules calf thymus DNA (ctDNA), bovine serum albumin (BSA), and human serum albumin (HSA) were assessed for complex formation and structural alterations in the biomolecules. UV\u2013Vis spectroscopy revealed surface plasmon resonance at 441\u2009nm. SEM analysis indicated a near\u2010Gaussian size distribution with a mean diameter of 0.44\u2009\u03bcm, while TEM confirmed the predominantly spherical morphology and polydispersity of the AgNPs within a size range of 10.5\u201332.9\u2009nm. XRD confirmed a face\u2010centered cubic (FCC) crystalline structure with an average size of 15.57\u2009nm, and EDS indicated the presence of silver, chlorine, oxygen, and nitrogen in the nanoparticle composition. TGA demonstrated the thermal stability of the nanoparticles up to 220\u00b0C. Bioassay experiments showed that AgNPs and cisplatin individually interacted with ct\u2010DNA to form stable complexes; the combined AgNP\u2010cisplatin system induced significant conformational changes in the DNA structure, indicating a synergistic effect. Similarly, BSA binding studies revealed stable complex formation with both AgNPs and the AgNP\u2010cisplatin combination, causing pronounced conformational changes in the protein. For HSA, the combination exhibited enhanced binding efficiency compared to individual agents, suggesting improved transport and bioavailability potential. Moreover, molecular docking studies demonstrated that the combined AgNP\u2010cisplatin system exhibited the lowest <jats:italic>E<\/jats:italic><jats:sub>total<\/jats:sub> of \u2212254\u2009kcal\/mol, compared to \u2212223.35\u2009kcal\/mol for AgNPs and \u2212112.30\u2009kcal\/mol for cisplatin alone. This indicates that the combined system interacts more strongly with ct\u2010DNA, likely within the minor groove, displaying enhanced binding affinity.<\/jats:p>","DOI":"10.1002\/jemt.24846","type":"journal-article","created":{"date-parts":[[2025,3,12]],"date-time":"2025-03-12T04:56:15Z","timestamp":1741755375000},"page":"2138-2158","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Green Synthesis of Silver Nanoparticles: Enhancing Cisplatin Binding to Biomolecules for Improved Drug Delivery and Therapeutic Synergy"],"prefix":"10.1002","volume":"88","author":[{"given":"Zainab","family":"Muhammad","sequence":"first","affiliation":[{"name":"Faculty of Science and Marine Environment Universiti Malaysia Terengganu  Kuala Terengganu Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5188-1130","authenticated-orcid":false,"given":"Mahboob","family":"Alam","sequence":"additional","affiliation":[{"name":"Department of Safety Engineering Dongguk University WISE  Gyeongju\u2010si Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3263-9568","authenticated-orcid":false,"given":"Nurul Huda Abd","family":"Kadir","sequence":"additional","affiliation":[{"name":"Faculty of Science and Marine Environment Universiti Malaysia Terengganu  Kuala Terengganu Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,3,11]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules28217352"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.3390\/nano12244494"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.colsurfb.2010.06.029"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules28165942"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jphotobiol.2017.05.015"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep20414"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1155\/2013\/936254"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010024\u201057972\u20105"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.3389\/fchem.2023.1273388"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.inoche.2023.111363"},{"issue":"3","key":"e_1_2_9_12_1","first-page":"1125","article-title":"UV\u2010Absorption Mechanisms of Ni 2+\u2010Binding Bovine Serum Albumin","volume":"7","author":"Dieaconu M.","year":"2012","journal-title":"Digest Journal of Nanomaterials and Biostructures"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11696\u2010020\u201001056\u2010x"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010022\u201026818\u20103"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1166\/jbn.2012.1451"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1080\/17518253.2024.2319246"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40486\u2010021\u201000131\u20106"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jphotobiol.2019.03.022"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joim.2018.10.002"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.3390\/ma15217695"},{"key":"e_1_2_9_21_1","first-page":"16","article-title":"PH\u00c2N L\u1eacP V\u00c0 NH\u1eacN DANH C\u1ea4U TR\u00daC M\u1ed8T CHALCONE T\u1eea D\u1ecaCH CHI\u1ebeT ETHYL ACETATE C\u1ee6A C\u00c2Y C\u1ece L\u00c0O\u2010Eupatorium ODORATUM L","volume":"8","author":"Lu\u00e2n N. 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