{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,28]],"date-time":"2026-07-28T12:47:50Z","timestamp":1785242870142,"version":"3.55.0"},"reference-count":56,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2025,3,16]],"date-time":"2025-03-16T00:00:00Z","timestamp":1742083200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Microscopy Res &amp;amp; Technique"],"published-print":{"date-parts":[[2025,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>The Papilionoideae is a taxonomically complex subfamily within the Fabaceae. Pollen characteristics are often used as additional diagnostic features in the taxonomy of Papilionoideae. Unfortunately, the pollen morphology of the Papilionoideae in India remains poorly understood. This study aims to bridge the palynological knowledge gap in the Papilionoideae taxa, focusing on Northeast India. In this investigation, light and scanning electron microscopy (SEM) were employed to examine the pollen morphology of 10 species belonging to nine genera of Papilionoideae: <jats:styled-content style=\"fixed-case\"><jats:italic>Aeschynomene americana<\/jats:italic><\/jats:styled-content>, <jats:styled-content style=\"fixed-case\"><jats:italic>Cajanus scarabaeoides<\/jats:italic><\/jats:styled-content>, <jats:styled-content style=\"fixed-case\"><jats:italic>Calopogonium mucunoides<\/jats:italic><\/jats:styled-content>, <jats:italic>Crotalaria calycina<\/jats:italic>, <jats:styled-content style=\"fixed-case\"><jats:italic>Crotalaria spectabilis<\/jats:italic><\/jats:styled-content>, <jats:italic>Grona triflora<\/jats:italic>, <jats:styled-content style=\"fixed-case\"><jats:italic>Lablab purpureus<\/jats:italic><\/jats:styled-content>, <jats:styled-content style=\"fixed-case\"><jats:italic>Pueraria phaseoloides<\/jats:italic><\/jats:styled-content>, <jats:styled-content style=\"fixed-case\"><jats:italic>Tephrosia candida<\/jats:italic><\/jats:styled-content>, and <jats:italic>Uraria crinita<\/jats:italic>. The pollen grains were prepared using Wodehouse's method; photomicrographs were taken to describe and measure the pollen traits. Pollen traits such as size, aperture number, and shape are stable traits, while exine sculptures, P\/E ratio, and gap between two adjacent colpi (GBC) traits can differentiate Papilionoideae taxa. Most of the species (nine species) had tricolporate pollen grains except <jats:styled-content style=\"fixed-case\"><jats:italic>Aeschynomene americana<\/jats:italic><\/jats:styled-content> with syncolporate pollen. Medium\u2010sized pollen was observed in most of the studied species (eight species), except with small\u2010sized pollen grains (<jats:styled-content style=\"fixed-case\"><jats:italic>A. americana<\/jats:italic><\/jats:styled-content>, <jats:styled-content style=\"fixed-case\"><jats:italic>C. calycina<\/jats:italic><\/jats:styled-content>). Three distinct pollen shapes were identified among the studied species: oblate\u2010spheroidal (six species), prolate\u2010spheroidal (three species), and prolate in <jats:italic>Uraria crinita<\/jats:italic>. Different types of exine sculpture observed were reticulate, fossulate, fossulate\u2010verrucate, perforate, foveolate\u2010reticulate, foveolate\u2010granulate, rugulate\u2010perforate, and psilate\u2010perforate. The first two principal component analysis (PCA) axis showed 60.3% of the total data variance. Polar axis (PA), equatorial diameter (ED), apocolpium, mesocolpium, and GBC, colpi breadth (ColpiB), P\/E, and nexine (&gt;\u20090.7) contribute significantly to the PCA. Further, a taxonomic key has been prepared based on the pollen morphological characters for correct species identification. This study strengthens and validates the subfamily's taxonomic status and will serve as a foundation for more phylogenetic and molecular research on Papilionoideae.<\/jats:p>","DOI":"10.1002\/jemt.24849","type":"journal-article","created":{"date-parts":[[2025,3,17]],"date-time":"2025-03-17T00:55:39Z","timestamp":1742172939000},"page":"2195-2209","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Contribution to the Palynology and Its Taxonomic Relevance in Selected Taxa of Papilionoideae (Fabaceae) From Northeast India"],"prefix":"10.1002","volume":"88","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2677-8422","authenticated-orcid":false,"given":"Sani","family":"Das","sequence":"first","affiliation":[{"name":"Plant Taxonomy and Biodiversity Laboratory, Department of Botany Tripura University  Suryamaninagar Tripura India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1632-5312","authenticated-orcid":false,"given":"Suman","family":"Paul","sequence":"additional","affiliation":[{"name":"Plant Taxonomy and Biodiversity Laboratory, Department of Botany Tripura University  Suryamaninagar Tripura India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Priyashi","family":"Kar","sequence":"additional","affiliation":[{"name":"Plant Taxonomy and Biodiversity Laboratory, Department of Botany Tripura University  Suryamaninagar Tripura India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marami","family":"Mili","sequence":"additional","affiliation":[{"name":"Plant Taxonomy and Biodiversity Laboratory, Department of Botany Tripura University  Suryamaninagar Tripura India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antara","family":"Bose","sequence":"additional","affiliation":[{"name":"Plant Taxonomy and Biodiversity Laboratory, Department of Botany Tripura University  Suryamaninagar Tripura India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Badal Kumar","family":"Datta","sequence":"additional","affiliation":[{"name":"Plant Taxonomy and Biodiversity Laboratory, Department of Botany Tripura University  Suryamaninagar Tripura India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,3,16]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1201\/b10256"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/jemt.24131"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/jemt.23701"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1080\/00173134.2022.2126727"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1080\/00173134.2019.1571626"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1590\/0001-3765202020181221"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/jemt.22971"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1080\/01916122.2022.2144521"},{"key":"e_1_2_11_10_1","first-page":"1","article-title":"Pollen Micromorphology of Selected Caesalpinioideae Taxa and Its Taxonomic Role","volume":"72","author":"Bahadur S.","year":"2024","journal-title":"Genetic Resources and Crop Evolution"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/jemt.24062"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/jemt.23188"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/tax.615011"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.sajb.2013.05.001"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1071\/BT98098"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.14719\/pst.2016.3.1.166"},{"key":"e_1_2_11_17_1","volume-title":"The Flora of Tripura State","author":"Deb D. B.","year":"1983"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.102.018150"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1890\/ES11-00147.1"},{"key":"e_1_2_11_20_1","doi-asserted-by":"crossref","unstructured":"Erdtman G.1969.\u201cHandbook of Palynology: Morphology Taxonomy Ecology.\u201dNo. 581.3325 E7.","DOI":"10.1163\/9789004631038"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.5194\/we-20-19-2020"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1080\/01916122.2021.1918279"},{"key":"e_1_2_11_23_1","first-page":"49","article-title":"Pollen Morphology in Diploid Species of Crotalaria L","volume":"88","author":"Gupta P. K.","year":"1979","journal-title":"Proceedings of the Indian Academy of Sciences\u2010Section B. Part 2, Plant Sciences"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-319-71365-6"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12870-023-04324-6"},{"key":"e_1_2_11_26_1","first-page":"1047","article-title":"Palynological Investigations on Some Selected Bee Forage Plants of Family Fabaceae Using Light and Scanning Electron Microscopy From Himachal Pradesh, India","volume":"21","author":"Jamwal R.","year":"2021","journal-title":"Plant Archives"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.1111\/nph.18024"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.3417\/2019337"},{"key":"e_1_2_11_29_1","doi-asserted-by":"publisher","DOI":"10.1002\/jemt.24097"},{"key":"e_1_2_11_30_1","doi-asserted-by":"publisher","DOI":"10.1002\/jemt.23405"},{"key":"e_1_2_11_31_1","doi-asserted-by":"publisher","DOI":"10.1080\/00173139209429451"},{"key":"e_1_2_11_32_1","doi-asserted-by":"publisher","DOI":"10.12705\/622.8"},{"key":"e_1_2_11_33_1","doi-asserted-by":"publisher","DOI":"10.12705\/661.3"},{"issue":"3","key":"e_1_2_11_34_1","first-page":"384","article-title":"A Palynological Study of the Genus Crotalaria L. (Leguminosae)","volume":"44","author":"Lin H. W.","year":"1999","journal-title":"Taiwania"},{"key":"e_1_2_11_35_1","doi-asserted-by":"publisher","DOI":"10.3897\/zookeys.651.10097"},{"key":"e_1_2_11_36_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-642-20992-5_1"},{"key":"e_1_2_11_37_1","doi-asserted-by":"publisher","DOI":"10.1038\/35002501"},{"key":"e_1_2_11_38_1","first-page":"4","article-title":"Vegan: Community Ecology Package","volume":"2","author":"Oksanen","year":"2017","journal-title":"R Package Version"},{"key":"e_1_2_11_39_1","doi-asserted-by":"publisher","DOI":"10.1111\/nph.20024"},{"key":"e_1_2_11_40_1","unstructured":"POWO.2024.\u201cPlants of the World Online.\u201d Facilitated by the Royal Botanic Gardens Kew. Published on the Internet.https:\/\/powo.science.kew.org\/."},{"key":"e_1_2_11_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.revpalbo.2006.06.008"},{"key":"e_1_2_11_42_1","unstructured":"R Core Team.2022.\u201cR: A Language and Environment for Statistical Computing.\u201dR Foundation for Statistical Computing Vienna Austria.https:\/\/www.R\u2010project.org\/."},{"issue":"13","key":"e_1_2_11_43_1","first-page":"1301","article-title":"Lesser Known Ethnomedicinal Plants of Mizoram, North East India: An Indo\u2010Burma Hotspot Region","volume":"4","author":"Rai P. K.","year":"2010","journal-title":"Journal of Medicinal Plants Research"},{"key":"e_1_2_11_44_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10661-011-1951-8"},{"key":"e_1_2_11_45_1","first-page":"1847","volume-title":"Floristic Diversity and Conservation Strategies in India","author":"Sanjappa M.","year":"2001"},{"key":"e_1_2_11_46_1","first-page":"300","volume-title":"Flowering Plants of India an Annotated Checklist (Dicotyledons)","author":"Sanjappa M.","year":"2020"},{"key":"e_1_2_11_47_1","doi-asserted-by":"publisher","DOI":"10.30848\/PJB2024\u20103(42)"},{"key":"e_1_2_11_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.flora.2024.152473"},{"key":"e_1_2_11_49_1","doi-asserted-by":"publisher","DOI":"10.54991\/jop.2022.538"},{"key":"e_1_2_11_50_1","doi-asserted-by":"publisher","DOI":"10.1080\/01916122.2023.2233586"},{"key":"e_1_2_11_51_1","doi-asserted-by":"publisher","DOI":"10.1080\/01916122.2022.2052993"},{"key":"e_1_2_11_52_1","doi-asserted-by":"publisher","DOI":"10.21105\/joss.01686"},{"key":"e_1_2_11_53_1","volume-title":"Pollen Grains","author":"Wodehouse R. P.","year":"1935"},{"key":"e_1_2_11_54_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0161762"},{"key":"e_1_2_11_55_1","doi-asserted-by":"publisher","DOI":"10.1002\/jemt.23999"},{"key":"e_1_2_11_56_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00606-015-1275-1"},{"key":"e_1_2_11_57_1","doi-asserted-by":"crossref","unstructured":"Zhao X. L. F.Wang J.Miao andS. M.Zhou.2023.\u201cPollen Morphology of Uraria Desv. and Christia Moench (Fabaceae) in China.\u201dAuthorea Preprints.","DOI":"10.22541\/au.168303789.95029188\/v1"}],"container-title":["Microscopy Research and Technique"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jemt.24849","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,16]],"date-time":"2025-10-16T16:24:41Z","timestamp":1760631881000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/jemt.24849"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,3,16]]},"references-count":56,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2025,8]]}},"alternative-id":["10.1002\/jemt.24849"],"URL":"https:\/\/doi.org\/10.1002\/jemt.24849","archive":["Portico"],"relation":{},"ISSN":["1059-910X","1097-0029"],"issn-type":[{"value":"1059-910X","type":"print"},{"value":"1097-0029","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,3,16]]},"assertion":[{"value":"2024-08-01","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-03-02","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-03-16","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}