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As PEGs are widely used in personal care products and pharmaceuticals, their increasing presence in marine ecosystems poses a potential threat to non\u2010target organisms. A total of 150 mussels were exposed to different concentrations of PEG (0.1\u2009mg\/L and 10\u2009mg\/L) over 14\u2009days. The impact of PEG exposure was assessed through cell viability assays, regulatory volume decrease (RVD) tests, and histological analysis, respectively, on hemolymph and digestive gland (DG) cells, on gills, DG, and gonads. Significant reductions in cell viability were observed in hemocytes and DG cells, particularly at higher PEG concentrations. Histological analysis revealed pronounced tissue damage, including hemocyte infiltration, lipofuscin aggregation, and epithelial disorganization in the gills, hepatopancreas, and gonads, indicating PEG\u2010induced cytotoxicity. The study also observed impaired RVD mechanisms in DG cells, highlighting changes in cell volume regulation. These findings demonstrate that PEG can induce significant physiological and morphological alterations in marine mussels, raising concerns about its impact on aquatic ecosystems.<\/jats:p>","DOI":"10.1002\/jemt.24854","type":"journal-article","created":{"date-parts":[[2025,3,17]],"date-time":"2025-03-17T03:40:02Z","timestamp":1742182802000},"page":"2222-2231","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["A Comparative Analysis of Physiological and Morphological Alteration in <scp><i>Mytilus galloprovincialis<\/i><\/scp> After Exposure to Polyethylene Glycol (<scp>PEG<\/scp>)"],"prefix":"10.1002","volume":"88","author":[{"given":"Federica","family":"Impellitteri","sequence":"first","affiliation":[{"name":"Department of Veterinary Sciences University of Messina  Messina Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cristiana 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