{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T14:45:52Z","timestamp":1786977952304,"version":"build-2736575974"},"reference-count":50,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2025,4,4]],"date-time":"2025-04-04T00:00:00Z","timestamp":1743724800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Microscopy Res &amp;amp; Technique"],"published-print":{"date-parts":[[2025,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Coccidiosis, caused by\n                    <jats:italic>Eimeria<\/jats:italic>\n                    spp., significantly impacts gastrointestinal health and leads to economic losses. This study investigated juglone, a phenolic compound, as a potential treatment for mouse coccidiosis caused by\n                    <jats:italic>Eimeria papillata<\/jats:italic>\n                    . Twenty\u2010five male Swiss albino mice were categorized into five groups: Group 1: non\u2010infected, Group 2: juglone control (JG), Group 3: infected with 1,000 sporulated oocysts, Group 4: infected\u2010treated with juglone (Infected\u2009+\u2009JG), and Group 5: infected\u2010treated with anticoccidial drug, amprolium (Infected\u2009+\u2009ACD). Following 5\u2009days of treatment, the mice were euthanized, and numerous analyses were performed such as growth performance, oocyst shedding, oxidative stress, histology, goblet cell response, and inflammation. Results showed that\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>E. papillata<\/jats:italic>\n                    <\/jats:styled-content>\n                    infection significantly decreased body weight and feed intake, increased oocyst shedding, and elevated oxidative stress markers while reducing antioxidant enzyme levels. Histopathological analysis indicated severe intestinal damage, with intracellular parasitic stages and reduced goblet cell density and MUC\u20102 expression. Infection also heightened gene expression of inflammatory cytokines including tumor necrosis factor\u2010alpha (TNF\u2010\u03b1), interferon\u2010gamma (IFN\u2010\u03b3), interleukin 1\u03b2 (IL1\u03b2), and interleukin 10 (IL10). Juglone treatment effectively mitigated these negative effects, improving body weight and feed consumption, lowering oocyst shedding, and restoring antioxidant enzyme activity. It also repaired intestinal structure, enhanced goblet cell density and MUC\u20102 expression, as well as diminished inflammatory cytokines. The findings indicated that juglone significantly mitigates the pathological effects induced by\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>E. papillata<\/jats:italic>\n                    <\/jats:styled-content>\n                    by decreasing parasite shedding, oxidative stress, inflammation, and modulating immune responses while facilitating intestinal recovery and enhancing overall health in infected mice.\n                  <\/jats:p>","DOI":"10.1002\/jemt.24871","type":"journal-article","created":{"date-parts":[[2025,4,5]],"date-time":"2025-04-05T18:28:44Z","timestamp":1743877724000},"page":"2429-2438","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Juglone as a Therapeutic Agent in Murine Coccidiosis: Enhancing Intestinal Recovery and Regulating Host Immune Responses"],"prefix":"10.1002","volume":"88","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8061-0014","authenticated-orcid":false,"given":"Shaimaa M.","family":"Kasem","sequence":"first","affiliation":[{"name":"Zoology Department Faculty of Science, Kafrelsheikh University  Kafr Elsheikh Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1869-5800","authenticated-orcid":false,"given":"Mohamed A.","family":"Dkhil","sequence":"additional","affiliation":[{"name":"Department of Zoology and Entomology Faculty of Science, Helwan University  Cairo Egypt"},{"name":"Applied Science Research Center Applied Science Private University  Amman Jordan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amal M.","family":"Marey","sequence":"additional","affiliation":[{"name":"Chemistry Department (Biotechnology Program), Faculty of Science Helwan University  Cairo Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rewaida","family":"Abdel\u2010Gaber","sequence":"additional","affiliation":[{"name":"Department of Zoology, College of Science King Saud University  Riyadh Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Felwa A.","family":"Thagfan","sequence":"additional","affiliation":[{"name":"Department of Biology, College of Science Princess Nourah Bint Abdulrahman University  Riyadh Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sherif","family":"Elshanat","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Faculty of Veterinary Medicine Alexandria University  Alexandria Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,4,4]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.21162\/pakjas\/23.184"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1177\/09731296241264060"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2023.1139899"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jksus.2020.03.016"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1080\/1828051X.2020.1815598"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.japr.2022.100314"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.3390\/antiox8040091"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.5829\/idosi.bjps.2017.80.86"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1590\/fst.123722"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.parint.2023.102741"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1590\/s1984\u201029612019068"},{"issue":"7","key":"e_1_2_11_13_1","first-page":"42","article-title":"Phenolics and Flavonoids Contents of Medicinal Plants, as Natural Ingredients for Many Therapeutic Purposes\u2010A Review","volume":"10","author":"Al\u2010Snafi A. 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