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The development of novel anticancer agents in combination with nanotechnology is a highly promising area as it could offer safer and more effective delivery methods for better overall outcomes. Considering this, a naturally occurring silkworm protein (sericin) was combined with nanoparticle formulations to explore the subsequent anticancer effects in breast cancer. In this study, a breast cancer animal mice model was developed by using 7, 12\u2010dimethylbenzneanthracene (DMBA) (a carcinogenic compound) followed by evaluation of the antitumor effects of a natural protein (sericin) and its nanoparticle conjugates. For this purpose, the mice were treated with two different concentrations of individual sericin protein together with conjugated silver nanoparticles (100\u2013200\u2009mg\/kg, b.w.). Mice were dissected after 60\u2009days of the treatment, and biochemical results showed that sericin and its conjugated nanoparticles had significant effects against the DMBA\u2010induced carcinogenic group in comparison with the untreated control group. Particularly, sericin\u2010conjugated NPs induced more prominent effects in the animal model. The histopathological analysis of the spleen and mammary tissues showed cytotoxic effects in the DMBA group, and sericin\u2010conjugated NPs were highly effective and minimized the cytotoxic effects of DMBA. Consequently, sericin\u2010conjugated nanoparticles induced more efficient anti\u2013breast tumor effects in animal mice models and have promising future clinical applications in overcoming drug resistance.<\/jats:p>","DOI":"10.1002\/jemt.24882","type":"journal-article","created":{"date-parts":[[2025,6,3]],"date-time":"2025-06-03T07:48:56Z","timestamp":1748936936000},"page":"2675-2689","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Biochemical and Histopathological Impact of <scp>DMBA<\/scp>\u2010Induced Breast Cancer and Therapeutic Potential of Silk Sericin and Sericin Silver Nanoparticles"],"prefix":"10.1002","volume":"88","author":[{"given":"Samaira","family":"Mumtaz","sequence":"first","affiliation":[{"name":"Medical Toxicology and Biochemistry Laboratory, Department of Zoology Government College University  Lahore Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shaukat","family":"Ali","sequence":"additional","affiliation":[{"name":"Medical Toxicology and Biochemistry Laboratory, Department of Zoology Government College University  Lahore Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Asim","family":"Pervaiz","sequence":"additional","affiliation":[{"name":"Human Genetics and Molecular Biology Department University of Health Sciences Lahore  Lahore Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Farooq","family":"Ahmad","sequence":"additional","affiliation":[{"name":"Department of Zoology The Islamia University of Bahawalpur  Bahawalpur Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Asma Abdul","family":"Latif","sequence":"additional","affiliation":[{"name":"Department of Zoology Lahore College for Women University  Lahore Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mah Noor","family":"Khan","sequence":"additional","affiliation":[{"name":"Department of Zoology University of Jhang  Jhang Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Muhammad Khalil Ahmad","family":"Khan","sequence":"additional","affiliation":[{"name":"Department of Zoology University of Okara  Okara Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Muhammad Adeeb","family":"Khan","sequence":"additional","affiliation":[{"name":"Green Hills Science College  Muzaffarabad Azad Jammu and Kashmir Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ali","family":"Hassan","sequence":"additional","affiliation":[{"name":"Medical Toxicology and Biochemistry Laboratory, Department of Zoology Government College University  Lahore Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rizwan","family":"Ullah","sequence":"additional","affiliation":[{"name":"Department of Zoology Mirpur University of Science and Technology (MUST), Mirpur\u201010250  AJK Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4815-2284","authenticated-orcid":false,"given":"Tafail Akbar","family":"Mughal","sequence":"additional","affiliation":[{"name":"Department of Zoology Women University of Azad Jammu &amp; 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