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We critically reviewed studies involving humans and animals, examining oral and dental tissues in\u00a0vivo and ex\u00a0vivo using label\u2010free NLOM. A scientometric analysis was also conducted to assess the 25\u2010year trajectory of NLOM in dentistry. A total of 78 studies from 17 countries were evaluated. Second harmonic generation (SHG) was the most commonly employed technique (83.3%), often combined with other NLOM methods (68%). The primary specimens imaged were the oral mucosa and teeth, with at least 70% of the studies contributing new morphological or biochemical insights and 34.6% generating data with potential clinical applications. SHG and cancer diagnosis were prominent descriptors, reflecting key findings from the most\u2010cited articles. Data indicate that NLOM provides robust and reliable clinical information, particularly for cancer and dental caries diagnosis; however, challenges remain in translating this knowledge into practice. Integrating this technology into clinical devices could advance dentistry by creating noninvasive tools for long\u2010term monitoring with minimal intervention and sample preparation.<\/jats:p>","DOI":"10.1002\/jemt.70012","type":"journal-article","created":{"date-parts":[[2025,7,9]],"date-time":"2025-07-09T14:59:03Z","timestamp":1752073143000},"page":"3050-3062","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Label\u2010Free Nonlinear Optical Microscopy: Shedding Light on Oral and Dental Tissues"],"prefix":"10.1002","volume":"88","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9623-7835","authenticated-orcid":false,"given":"Tarc\u00edlia Aparecida","family":"Silva","sequence":"first","affiliation":[{"name":"Department of Oral Surgery, Pathology, and Clinical Dentistry, School of Dentistry Universidade Federal de Minas Gerais  Belo Horizonte Brazil"},{"name":"Centre de Recherche Des Cordeliers, Laboratory of Molecular Oral Pathophysiology Universit\u00e9 de Paris, Sorbonne Universit\u00e9, Inserm  Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9 Alcides Almeida","family":"de Arruda","sequence":"additional","affiliation":[{"name":"Department of Oral Surgery, Pathology, and Clinical Dentistry, School of Dentistry Universidade Federal de Minas Gerais  Belo Horizonte Brazil"},{"name":"Department of Oral Diagnosis and Pathology, School of Dentistry Universidade Federal do Rio de Janeiro  Rio de Janeiro Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nayara Concei\u00e7\u00e3o Marcos","family":"Santana","sequence":"additional","affiliation":[{"name":"Department of Oral Surgery, Pathology, and Clinical Dentistry, School of Dentistry Universidade Federal de Minas Gerais  Belo Horizonte Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Renan Souza","family":"Cunha","sequence":"additional","affiliation":[{"name":"S\u00e3o Carlos Institute of Physics Universidade de S\u00e3o Paulo  S\u00e3o Carlos Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Victor Zanetti","family":"Drumond","sequence":"additional","affiliation":[{"name":"Department of Oral Surgery, Pathology, and Clinical Dentistry, School of Dentistry Universidade Federal de Minas Gerais  Belo Horizonte Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leandro Malard","family":"Moreira","sequence":"additional","affiliation":[{"name":"Departament of Physics Universidade Federal de Minas Gerais  Belo Horizonte Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bruno Augusto Benevenuto de","family":"Andrade","sequence":"additional","affiliation":[{"name":"Department of Oral Diagnosis and Pathology, School of Dentistry Universidade Federal do Rio de Janeiro  Rio de Janeiro Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juliane","family":"Isaac","sequence":"additional","affiliation":[{"name":"Centre de Recherche Des Cordeliers, Laboratory of Molecular Oral Pathophysiology Universit\u00e9 de Paris, Sorbonne Universit\u00e9, Inserm  Paris France"},{"name":"Department of Oral Biology, Dental Faculty Universit\u00e9 de Paris  Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicolas","family":"Dupr\u00e9","sequence":"additional","affiliation":[{"name":"Centre de Recherche Des Cordeliers, Laboratory of Molecular Oral Pathophysiology Universit\u00e9 de Paris, Sorbonne Universit\u00e9, Inserm  Paris France"},{"name":"Department of Oral Biology, Dental Faculty Universit\u00e9 de Paris  Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benjamin P. 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