{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T16:01:13Z","timestamp":1787068873450,"version":"build-2736575974"},"reference-count":64,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2025,6,20]],"date-time":"2025-06-20T00:00:00Z","timestamp":1750377600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Microscopy Res &amp; Technique"],"published-print":{"date-parts":[[2025,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>The behavior and incidence of the gadolinium (Gd) in the organism, has been carried out because of its increasing industrial and medical uses. Gadolinium was intraperitoneally administered to adult male Wistar rats, allowing access to organs via the blood. First, the nutritional status was daily determined using body weight and food intake of animals. Second, testicles, seminal vesicles, epididymides and their caudae, as well as liver, kidneys and brain weights were recorded. Third, treated and control organs were prepared for ultrastructural and microanalytical observations and were used for Gd quantification by ICP\u2010MS technique. Seminal vesicles and the kidneys of treated rats showed significant increase in absolute weight. The ICP\u2010MS showed that Gd was found in very important amounts in liver, testicle and urines collected over 24\u2009h. The ultrastructural and microanalytical techniques showed that Gd was found in the lysosomes of Sertoli, Leydig and hepatic cells in the form of electron\u2010dense grains associated with phosphorus. Ultrastructural alterations were also noticed in those cells. These original results showed that the lysosome was certainly exceeded since Gd was highly detected in many organs like liver and testicles. The presence of Gd in the rat organism seemed to cause toxicity and alteration in testicular cell organelles leading very probably to disruption in the reproductive tract functions. In this case, the organism seemed to activate elimination of Gd via hepatic biliary ducts and kidney excretion confirmed by the presence of Gd in the different analyzed liver territories and in the collected urines.<\/jats:p>","DOI":"10.1002\/jemt.70015","type":"journal-article","created":{"date-parts":[[2025,6,20]],"date-time":"2025-06-20T03:19:42Z","timestamp":1750389582000},"page":"2744-2753","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Gadolinium and Its Effects on Nutritional Status, Microscopy and Microanalytical Outcomes: Quantification by ICP\u2010MS and Incidence on Reproductive Tract and Liver of Wistar Rats"],"prefix":"10.1002","volume":"88","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4623-231X","authenticated-orcid":false,"given":"Samira","family":"Maghraoui","sequence":"first","affiliation":[{"name":"Laboratory of Physiology Faculty of Medicine of Tunis  Tunis Tunisia"},{"name":"Laboratory of Medical Biology Abderahmen Mami Hospital of Pneumology\u2010Phtisiology  Ariana Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adrian","family":"Florea","sequence":"additional","affiliation":[{"name":"Department of Cell and Molecular Biology Faculty of Medicine, \u201cIuliu Hatieganu\u201d University of Medicine and Pharmacy  Cluj\u2010Napoca Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leila","family":"Tekaya","sequence":"additional","affiliation":[{"name":"Laboratory of Physiology Faculty of Medicine of Tunis  Tunis Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,6,20]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.crvi.2008.02.006"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12011\u2010008\u20108121\u2010z"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/jemt.20628"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jtemb.2017.09.011"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12011\u2010017\u20101135\u20107"},{"issue":"1","key":"e_1_2_11_7_1","first-page":"1016","article-title":"Gold Deposits Within Lysosomes of Placental Cells: Ultrastructural and Quantitative Study","volume":"3","author":"Badri N.","year":"2020","journal-title":"Annali di Ostetricia, Ginecologia, Medicina Perinatale"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.4172\/2329\u20106577.1000186"},{"issue":"3","key":"e_1_2_11_9_1","first-page":"1022","article-title":"Lysosomes of Brain Cells: A Site of Handling of Lanthanum","volume":"2","author":"Badri N.","year":"2019","journal-title":"American Journal of Pharmacology"},{"issue":"3","key":"e_1_2_11_10_1","first-page":"395","article-title":"The Role of Lysosomes in the Selective Concentration of Mineral Elements. A Microanalytical Study","volume":"42","author":"Berry J. P.","year":"1996","journal-title":"Cellular and Molecular Biology (Noisy\u2010le\u2010Grand, France)"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/and.13031"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pan.2012.12.002"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejrad.2008.02.011"},{"issue":"1","key":"e_1_2_11_14_1","first-page":"46","article-title":"Effects of Gadolinium Depositions In\u00a0Vivo","volume":"93","author":"Bruno D.","year":"2021","journal-title":"Radiologic Technology"},{"key":"e_1_2_11_15_1","first-page":"395","article-title":"Microanalyse Par \u00c9mission Ionique Secondaire","volume":"1","author":"Castaing R.","year":"1960","journal-title":"Journal of Microscopy"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1039\/b905145g"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.3967\/bes2018.047"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1001\/jamadermatol.2014.2660"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jtemb.2016.08.007"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1037\/h0077264"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.rli.0000112789.57341.01"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1093\/ndt\/gfk062"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/0730\u2010725x(93)90194\u2010i"},{"key":"e_1_2_11_24_1","unstructured":"Hocine N. J. P.Berry F.Escaig andP.Galle.1994.Mapping and Localization of Gadolinium. A Study Using Ion Microanalysis and Electron MicroprobeLes \u00e9ditions de Physique 841\u2013842."},{"issue":"2","key":"e_1_2_11_25_1","first-page":"271","article-title":"Subcellular Localization of Gadolinium Injected as Soluble Salt in Rats: A Microanalytical Study","volume":"41","author":"Hocine N.","year":"1995","journal-title":"Cellular and Molecular Biology"},{"key":"e_1_2_11_26_1","unstructured":"Hocine N. J. P.Berry M. H.Jaafoura andP.Galle.1994.\u201cIntracellular Localization of Gadolinium Lanthanum and Terbium. Three Rare Earths. A Microanalytical Study Using Ion Microanalysis and Electron Microprobe Metal Ion in Biology and Medicine.\u201dLes \u00e9ditions de Physique 193\u2013198."},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.3174\/ajnr.A1205"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mri.2016.08.024"},{"key":"e_1_2_11_29_1","doi-asserted-by":"publisher","DOI":"10.1098\/rspb.1950.0065"},{"key":"e_1_2_11_30_1","doi-asserted-by":"publisher","DOI":"10.54203\/scil.2022.wvj37"},{"key":"e_1_2_11_31_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010023\u201036991\u20108"},{"key":"e_1_2_11_32_1","doi-asserted-by":"publisher","DOI":"10.1097\/RLI.0000000000000344"},{"key":"e_1_2_11_33_1","doi-asserted-by":"publisher","DOI":"10.1093\/jmicro\/dfr009"},{"key":"e_1_2_11_34_1","doi-asserted-by":"publisher","DOI":"10.1093\/jmicro\/dft002"},{"key":"e_1_2_11_35_1","doi-asserted-by":"publisher","DOI":"10.1002\/jemt.20945"},{"key":"e_1_2_11_36_1","doi-asserted-by":"publisher","DOI":"10.1556\/APhysiol.100.2013.021"},{"key":"e_1_2_11_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jtemb.2022.126997"},{"key":"e_1_2_11_38_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12011\u2010022\u201003180\u2010z"},{"key":"e_1_2_11_39_1","first-page":"33","article-title":"Localisation et Incidences Subcellulaires du Gadolinium au Niveau Des Testicules et du Foie de Rat: Etude Histologique, Ultrastructurale et Microanalytique","author":"Maghraoui S.","year":"2024","journal-title":"Revue FSB"},{"issue":"3","key":"e_1_2_11_40_1","first-page":"195","article-title":"R\u00f4les Des Lysosomes Dans le Comportement Intracellulaire D'\u00e9l\u00e9ments Min\u00e9raux: Cas du Lanthane","volume":"78","author":"Manoubi\u2010Tekaya L.","year":"2000","journal-title":"Tunis M\u00e9dical"},{"key":"e_1_2_11_41_1","doi-asserted-by":"publisher","DOI":"10.1212\/01.WNL.0000156805.45547.6E"},{"key":"e_1_2_11_42_1","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.2006060601"},{"key":"e_1_2_11_43_1","doi-asserted-by":"publisher","DOI":"10.1148\/rg.2020190110"},{"key":"e_1_2_11_44_1","doi-asserted-by":"publisher","DOI":"10.1148\/radiol.2016152846"},{"key":"e_1_2_11_45_1","doi-asserted-by":"publisher","DOI":"10.1148\/radiol.2017161594"},{"issue":"11","key":"e_1_2_11_46_1","first-page":"41","article-title":"The Transplacental Passage of Gadolinium and the Ultrastructural Modifications in Placenta Tissues of Rats","volume":"8","author":"Mhamdi M.","year":"2019","journal-title":"EC Gynaecology"},{"issue":"6","key":"e_1_2_11_47_1","first-page":"238","article-title":"The Effects of Gadolinium in the Uterus and in the Ovary of Pregnant Rat: Ultrastructural Study","volume":"5","author":"Mhamdi M.","year":"2017","journal-title":"EC Gynaecology"},{"key":"e_1_2_11_48_1","doi-asserted-by":"publisher","DOI":"10.1542\/peds.2015\u20102222"},{"key":"e_1_2_11_49_1","doi-asserted-by":"publisher","DOI":"10.1097\/00004424\u2010199402000\u201000017"},{"key":"e_1_2_11_50_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0231495"},{"key":"e_1_2_11_51_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0003\u20104509(06)75324\u2010X"},{"key":"e_1_2_11_52_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1773\u2010035X(21)00171\u20104"},{"key":"e_1_2_11_53_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mri.2016.09.005"},{"key":"e_1_2_11_54_1","doi-asserted-by":"publisher","DOI":"10.1002\/cld.1024"},{"key":"e_1_2_11_55_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.braindev.2015.08.009"},{"key":"e_1_2_11_56_1","doi-asserted-by":"publisher","DOI":"10.1097\/00004424\u2010199406001\u201000051"},{"key":"e_1_2_11_57_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.forsciint.2007.06.026"},{"key":"e_1_2_11_58_1","doi-asserted-by":"publisher","DOI":"10.2214\/AJR.15.15842"},{"key":"e_1_2_11_59_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0378\u20104320(03)00100\u20103"},{"key":"e_1_2_11_60_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrm.1115"},{"key":"e_1_2_11_61_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijrefrig.2010.12.009"},{"key":"e_1_2_11_62_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.heliyon.2024.e27419"},{"key":"e_1_2_11_63_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0720\u2010048x(02)00332\u20107"},{"key":"e_1_2_11_64_1","doi-asserted-by":"publisher","DOI":"10.1039\/C2MD00279E"},{"key":"e_1_2_11_65_1","doi-asserted-by":"publisher","DOI":"10.1118\/1.1644518"}],"container-title":["Microscopy Research and Technique"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jemt.70015","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,16]],"date-time":"2025-10-16T15:57:22Z","timestamp":1760630242000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/jemt.70015"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,6,20]]},"references-count":64,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2025,10]]}},"alternative-id":["10.1002\/jemt.70015"],"URL":"https:\/\/doi.org\/10.1002\/jemt.70015","archive":["Portico"],"relation":{},"ISSN":["1059-910X","1097-0029"],"issn-type":[{"value":"1059-910X","type":"print"},{"value":"1097-0029","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,6,20]]},"assertion":[{"value":"2025-04-17","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-06-11","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-06-20","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}