{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T13:27:48Z","timestamp":1787318868750,"version":"build-2736575974"},"reference-count":59,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2025,7,16]],"date-time":"2025-07-16T00:00:00Z","timestamp":1752624000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,7,16]],"date-time":"2025-07-16T00:00:00Z","timestamp":1752624000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Microscopy Res &amp; Technique"],"published-print":{"date-parts":[[2025,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Herein, we adapted a quick, cost\u2010effective, and environmentally friendly biological method for the synthesis of Ag\u2010NPs using silver nitrate solution as a precursor, and the aqueous leaf extract of\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Lespedeza juncea<\/jats:italic>\n                    <\/jats:styled-content>\n                    (Chinese lespedeza) was exploited to synthesize Ag\u2010NPs. Various physiochemical characterization techniques were used to characterize the nanoparticles. The UV spectrum revealed a resonance absorption peak at 428\u2009nm, indicating successful synthesis of the nanoparticles. The EDX results indicated the presence of Ag, C, O, and Cl elements in biosynthesized Ag\u2010NPs with elemental compositions of 53.93%, 32.34%, 12.49%, and 1.23%, respectively. The XRD analysis displayed the crystalline nature of the nanoparticles with a face\u2010centered cubic lattice. The FTIR spectrum confirmed the involvement of plant\u2010based biological compounds as reducing and capping agents. TEM revealed quasi\u2010spherical Ag\u2010NPs of 50\u2009nm or smaller in size. An insight into its biological activities reveals significant antimicrobial activities against\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Staphylococcus aureus<\/jats:italic>\n                    <\/jats:styled-content>\n                    (14.1\u2009\u00b1\u20090.76\u2009mm),\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Pseudomonas aeruginosa<\/jats:italic>\n                    <\/jats:styled-content>\n                    (11.50\u2009\u00b1\u20090.40\u2009mm),\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Escherichia coli<\/jats:italic>\n                    <\/jats:styled-content>\n                    (16\u2009\u00b1\u20090.95\u2009mm), and the fungal strains, viz.\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Candida albicans<\/jats:italic>\n                    <\/jats:styled-content>\n                    (14\u2009\u00b1\u20090.80\u2009mm),\n                    <jats:italic>Aspergillus flavus<\/jats:italic>\n                    (16.50\u2009\u00b1\u20090.50\u2009mm),\n                    <jats:italic>Aspergillus niger<\/jats:italic>\n                    (17\u2009\u00b1\u20090.86\u2009mm) and afflicted 75% mortality to\n                    <jats:italic>\n                      <jats:styled-content style=\"fixed-case\">Caenorhabditis<\/jats:styled-content>\n                      elegans\n                    <\/jats:italic>\n                    . Furthermore, the Ag\u2010NPs were found to be potent inhibitors of the enzymes tyrosinase, urease, acetylcholinesterase, and butyrylcholinesterase with IC\n                    <jats:sub>50<\/jats:sub>\n                    values of 14.3\u2009\u00b1\u20090.2, 19.5\u2009\u00b1\u20091.1, 9.3\u2009\u00b1\u20091.3, and 32.65\u2009\u00b1\u20091.9\u2009\u03bcg\/mL, respectively. The overall outcome of the study suggests that\n                    <jats:italic>L. juncea<\/jats:italic>\n                    mediated synthesized Ag\u2010NPs hold the potential to be employed as a promising tool for their antibacterial, antifungal, nematocidal, and for a variety of enzymes inhibitory activities.\n                  <\/jats:p>","DOI":"10.1002\/jemt.70040","type":"journal-article","created":{"date-parts":[[2025,7,17]],"date-time":"2025-07-17T04:25:20Z","timestamp":1752726320000},"page":"3171-3181","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Green Synthesis and Characterization of Silver Nanoparticles Using\n                    <scp>\n                      <i>Lespedeza juncea<\/i>\n                    <\/scp>\n                    Extract: An Insight Into Its Antibacterial, Antifungal, and Enzyme Inhibitory Potential"],"prefix":"10.1002","volume":"88","author":[{"given":"Muhammad","family":"Irshad","sequence":"first","affiliation":[{"name":"Department of Biochemistry, Faculty of Biological and Health Sciences Hazara University  Mansehra Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0562-4950","authenticated-orcid":false,"given":"Luqman","family":"Shah","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Faculty of Biological and Health Sciences Hazara University  Mansehra Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nodia","family":"Shujaat","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Faculty of Biological and Health Sciences Hazara University  Mansehra Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Majid","family":"Khan","sequence":"additional","affiliation":[{"name":"College of Chemistry, Fuzhou University  Fuzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zeeshan","family":"Niaz","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Faculty of Biological and Health Sciences Hazara University  Mansehra Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7070-9053","authenticated-orcid":false,"given":"Ikram","family":"Ullah","sequence":"additional","affiliation":[{"name":"Department of Biotechnology and Genetic Engineering, Faculty of Biological and Health Sciences Hazara University  Mansehra Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,7,16]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.surfin.2019.100359"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.15666\/aeer\/1603_26512662"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1155\/2019\/8642303"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40643-014-0003-y"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.inoche.2021.108647"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.2147\/IJN.S193679"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.21307\/jofnem-2020-002"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1093\/ajcp\/45.4_ts.493"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.scp.2021.100426"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1515\/gps-2020-0012"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1080\/24701556.2021.2025078"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.rinp.2017.07.044"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4020-8455-3_29"},{"issue":"2","key":"e_1_2_10_15_1","doi-asserted-by":"crossref","first-page":"49","DOI":"10.16955\/bitkorb.485072","article-title":"Effects of Silver Nanoparticle (AgNPs) Applications Prepared Using Lantana Camara L. 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