{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,18]],"date-time":"2026-03-18T17:11:13Z","timestamp":1773853873863,"version":"3.50.1"},"reference-count":46,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2025,11,14]],"date-time":"2025-11-14T00:00:00Z","timestamp":1763078400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,11,14]],"date-time":"2025-11-14T00:00:00Z","timestamp":1763078400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100000038","name":"Natural Sciences and Engineering Research Council of Canada","doi-asserted-by":"publisher","award":["#2018\u201006489"],"award-info":[{"award-number":["#2018\u201006489"]}],"id":[{"id":"10.13039\/501100000038","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Microscopy Res &amp; Technique"],"published-print":{"date-parts":[[2026,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    To enhance the longevity of resin sealants on the occlusal surfaces of posterior teeth, improvements are needed in antibacterial properties, mechanical resistance, and bond strength to enamel. This study investigated the effectiveness of incorporating epigallocatechin\u2010gallate methacrylate (EGCG\u2010M) into a pit and fissure sealant (SEAL, PacSeal) by assessing its penetration depth, degree of conversion, color properties, and surface morphology. Thirty blocks of bovine enamel were divided into three groups: SEAL (control), SEAL with neat EGCG (E0), and SEAL with EGCG\u2010M. Analyses were conducted including penetration depth with confocal microscopy, degree of conversion using FTIR\u2010ATR, color analysis using CIELab, and surface morphology via 3D laser microscope. The EGCG\u2010M group exhibited the highest degree of conversion (44.9%\u2009\u00b1\u20092.1%) compared to the SEAL (41.7%\u2009\u00b1\u20092.1%) and E0 (42.1%\u2009\u00b1\u20091.1%) groups (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u20090.040). Deeper penetration was observed in the EGCG\u2010M group (9.7\u2009\u00b1\u200910\u2009\u03bcm), followed by SEAL (\u22120.1\u2009\u00b1\u200912.2\u2009\u03bcm) and E0 (\u22125.3\u2009\u00b1\u20099.7\u2009\u03bcm) groups (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u20090.002). Although no significant difference was found in\n                    <jats:italic>L<\/jats:italic>\n                    * (lightness) coordinate values between the groups (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u20090.060), the EGCG\u2010M group exhibited a more heterogeneous surface. Therefore, incorporating EGCG methacrylate into the sealant improved the degree of conversion and penetration capability without notable changes in color.\n                  <\/jats:p>","DOI":"10.1002\/jemt.70091","type":"journal-article","created":{"date-parts":[[2025,11,14]],"date-time":"2025-11-14T11:21:52Z","timestamp":1763119312000},"page":"557-564","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Epigallocatechin\u2010Gallate Methacrylate as a Novel Addition to Pit and Fissure Sealants: An In\u00a0Vitro Analysis of Physical Properties and Microscopy"],"prefix":"10.1002","volume":"89","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9298-1114","authenticated-orcid":false,"given":"Alyssa Teixeira","family":"Obeid","sequence":"first","affiliation":[{"name":"Department of Operative Dentistry, Endodontics and Dental Materials, Bauru School of Dentistry University of S\u00e3o Paulo  Bauru S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marilia Mattar","family":"Amo\u00eado Campos Velo","sequence":"additional","affiliation":[{"name":"Department of Restorative Dentistry, School of Dentistry S\u00e3o Paulo State University\u2014UNESP  Araraquara S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9299-1304","authenticated-orcid":false,"given":"Karin","family":"Landmayer","sequence":"additional","affiliation":[{"name":"Department of Operative Dentistry, School of Dentistry University of S\u00e3o Paulo  S\u00e3o Paulo S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniela Alvim","family":"Chrisostomo","sequence":"additional","affiliation":[{"name":"Restorative Discipline, Dental Research Institute, Faculty of Dentistry University of Toronto  Toronto Ontario Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carlos Alberto Spironelli","family":"Ramos","sequence":"additional","affiliation":[{"name":"Endodontics Department State University of Londrina  Londrina Paran\u00e1 Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rafael Francisco Lia","family":"Mondelli","sequence":"additional","affiliation":[{"name":"Department of Operative Dentistry, Endodontics and Dental Materials, Bauru School of Dentistry University of S\u00e3o Paulo  Bauru S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paulo Henrique","family":"dos Santos","sequence":"additional","affiliation":[{"name":"Restorative Discipline, Dental Research Institute, Faculty of Dentistry University of Toronto  Toronto Ontario Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anuradha","family":"Prakki","sequence":"additional","affiliation":[{"name":"Restorative Discipline, Dental Research Institute, Faculty of Dentistry University of Toronto  Toronto Ontario Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juliana Fraga Soares","family":"Bombonatti","sequence":"additional","affiliation":[{"name":"Department of Operative Dentistry, Endodontics and Dental Materials, Bauru School of Dentistry University of S\u00e3o Paulo  Bauru S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,11,14]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/14651858.CD001830.pub5"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.5005\/jp-journals-10024-2715"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00784\u2010011\u20100549\u20104"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.3390\/ma14051056"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cden.2008.09.003"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/idj.12191"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.dental.2021.09.005"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1067\/mpr.2002.121743"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.5005\/jp\u2010journals\u201010005\u20101742"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1159\/000448662"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/jicd.12203"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.dental.2025.05.006"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajodo.2006.10.014"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1093\/ejo\/cjm020"},{"key":"e_1_2_11_16_1","first-page":"144","article-title":"Oligomeric Proanthocyanidin Complexes: History, Structure, and Phytopharmaceutical Applications","volume":"5","author":"Fine A. 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