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Despite its economic significance, detailed knowledge of its reproductive anatomy and egg ultrastructure has remained limited, constraining the development of advanced molecular control strategies such as CRISPR\/Cas9\u2010based genome editing. The present study provides the first comprehensive characterization of the reproductive system and egg surface morphology of\n                    <jats:italic>P. gossypiella<\/jats:italic>\n                    using stereomicroscopy and scanning electron microscopy (SEM) techniques. The male reproductive system consists of fused, bean\u2010shaped testes, seminal vesicles, duplex and simplex ejaculatory ducts, and paired accessory glands. The female reproductive system comprises paired ovaries with four polytrophic ovarioles per ovary, lateral and common oviducts, accessory glands, corpus bursae, and spermathecal glands. Eggs are oval, dorsoventrally flattened, exhibit a reticulated chorion with distinct micropylar and aeropylar regions. SEM images revealed 6\u20139 rosette cells encircling a circular micropylar plate, 14\u201319 first order and 17\u201323\u2009s order ribs, and 250\u2013291 polygonal surface cells. The structural features of\n                    <jats:italic>P. gossypiella<\/jats:italic>\n                    eggs reveal key sites for sperm entry, aeropylar respiration, and candidate zones for microinjection in gene editing applications. These findings establish a morphological baseline critical for optimizing embryo manipulation and ribonucleoprotein (RNP) delivery in lepidopteran genome editing. This study represents a pioneering effort to integrate classical egg morphology with molecular entomology, thereby advancing precision genetic interventions aimed at resistance management and population suppression in\n                    <jats:italic>P. gossypiella<\/jats:italic>\n                    .\n                  <\/jats:p>","DOI":"10.1002\/jemt.70154","type":"journal-article","created":{"date-parts":[[2026,5,6]],"date-time":"2026-05-06T05:52:08Z","timestamp":1778046728000},"page":"1218-1229","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Ultrastructural Insights Into the Reproductive Anatomy and Eggs of Cotton Pink Bollworm,\n                    <i>Pectinophora gossypiella<\/i>\n                    Saunders (Lepidoptera: Gelechiidae)"],"prefix":"10.1002","volume":"89","author":[{"ORCID":"https:\/\/orcid.org\/0009-0000-4529-8072","authenticated-orcid":false,"given":"K.","family":"Logeswaran","sequence":"first","affiliation":[{"name":"Division of Genomic Resources ICAR \u2013 National Bureau of Agricultural Insect Resources  Bengaluru Karnataka India"},{"name":"Division of Crop Protection Indian Institute of Horticultural Research  Bengaluru Karnataka India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4678-8396","authenticated-orcid":false,"given":"Suresh R.","family":"Jambagi","sequence":"additional","affiliation":[{"name":"Division of Genomic Resources ICAR \u2013 National Bureau of Agricultural Insect Resources  Bengaluru Karnataka India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vinithashri","family":"Gautam","sequence":"additional","affiliation":[{"name":"Division of Genomic Resources ICAR \u2013 National Bureau of Agricultural Insect Resources  Bengaluru Karnataka India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T.","family":"Venkatesan","sequence":"additional","affiliation":[{"name":"Division of Genomic Resources ICAR \u2013 National Bureau of Agricultural Insect Resources  Bengaluru Karnataka India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D.","family":"Sagar","sequence":"additional","affiliation":[{"name":"Division of Genomic Resources ICAR \u2013 National Bureau of Agricultural Insect Resources  Bengaluru Karnataka India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S. N.","family":"Sushil","sequence":"additional","affiliation":[{"name":"Division of Genomic Resources ICAR \u2013 National Bureau of Agricultural Insect Resources  Bengaluru Karnataka India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2327-6988","authenticated-orcid":false,"given":"M.","family":"Mohan","sequence":"additional","affiliation":[{"name":"Division of Genomic Resources ICAR \u2013 National Bureau of Agricultural Insect Resources  Bengaluru Karnataka India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,5,5]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1163\/221194312X651391"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1017\/CBO9780511818202"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1080\/01651269.1980.10553369"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.4039\/Ent1247-1"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.11646\/zootaxa.4442.3.10"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.11646\/zootaxa.4085.3.3"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.1515\/9783112742150"},{"key":"e_1_2_12_9_1","doi-asserted-by":"publisher","DOI":"10.1242\/jeb.201.16.2425"},{"key":"e_1_2_12_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10709-009-9358-y"},{"key":"e_1_2_12_11_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12864-023-09905-1"},{"key":"e_1_2_12_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12600\u2010019\u201000738\u2010x"},{"key":"e_1_2_12_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/0020\u20107322(87)90021\u20103"},{"key":"e_1_2_12_14_1","doi-asserted-by":"publisher","DOI":"10.1093\/aesa\/68.3.417"},{"key":"e_1_2_12_15_1","doi-asserted-by":"publisher","DOI":"10.1093\/AESA\/SAU001"},{"key":"e_1_2_12_16_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.125054"},{"key":"e_1_2_12_17_1","doi-asserted-by":"publisher","DOI":"10.4161\/fly.26566"},{"key":"e_1_2_12_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biocontrol.2025.105822"},{"key":"e_1_2_12_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jip.2025.108452"},{"key":"e_1_2_12_20_1","first-page":"1052","volume-title":"Insect Reproductive System (Chinese Edition)","author":"Ji B.\u2010Z.","year":"2014"},{"key":"e_1_2_12_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jobaz.2015.09.004"},{"key":"e_1_2_12_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010018\u201007964\u20107"},{"key":"e_1_2_12_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010025\u201089575\u2010z"},{"key":"e_1_2_12_24_1","doi-asserted-by":"publisher","DOI":"10.1006\/anbe.1999.1294"},{"key":"e_1_2_12_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aspen.2018.05.011"},{"key":"e_1_2_12_26_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.en.21.010176.001215"},{"key":"e_1_2_12_27_1","volume-title":"Insect Ovary Development and Pest Forecast","author":"Li R. 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