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Microfluidic shear flow assays provide a highly sensitive tool for studying tumor cell communication at the single cell level with the microenvironment. Adhesion thereby serves as a functional readout that reflects cellular state, including loss of viability. However, previous platforms required extensive manual handling and time\u2010consuming post\u2010assay analysis. We developed a bright\u2010field microscopy\u2010enabled, semi\u2010automated shear flow platform that combines hardware operation with a machine\u2010learning\u2010based analysis pipeline. 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