{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T02:16:53Z","timestamp":1790302613848,"version":"4.1.0"},"reference-count":54,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2025,7,27]],"date-time":"2025-07-27T00:00:00Z","timestamp":1753574400000},"content-version":"vor","delay-in-days":26,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,7,1]],"date-time":"2025-07-01T00:00:00Z","timestamp":1751328000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["esskajournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J. exp. orthop."],"published-print":{"date-parts":[[2025,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Purpose<\/jats:title>\n                    <jats:p>Purpose of the present study is to report a modified protocol for stromal vascular fraction (SVF) isolation from abdominal fat, analyse its cellular composition and gene expression profile, and verify the safety and feasibility of the harvesting\u2010preparation technique and subsequent intra\u2010articular knee injection.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>The SVF was obtained after mechanical dissociation of the autologous harvested adipose tissue. It was combined with autologous platelet\u2010rich plasma and subsequently intra\u2010articularly injected into both knees of patients with osteoarthritis. Part of the SVF solution was used to analyse its cellular composition via flow cytometry and its gene expression profile via real\u2010time polymerase chain reaction. Any postinjection complications were documented.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Twenty\u2010three patients (10 female; 62.4\u2009\u00b1\u20098.4 years old) were enrolled. Posttreatment adverse events were mild and spontaneously resolved. Patients needed 3.7\u2009\u00b1\u20091.3 days for their knee to feel the same as before the injection. No patient provided less than 60\u2009mL of lipoaspirate. Per 1\u2009mL of SVF the total amount of viable nucleated cells was 49.7\u2009\u00b1\u200922.9\u2009\u00d7\u200910\n                      <jats:sup>6<\/jats:sup>\n                      on average, including 44.5\u2009\u00b1\u200923.2\u2009\u00d7\u200910\n                      <jats:sup>6<\/jats:sup>\n                      CD90+\/CD105+ adipose\u2010derived stem cells, 0.54\u2009\u00b1\u20090.18\u2009\u00d7\u200910\n                      <jats:sup>6<\/jats:sup>\n                      hematopoietic stem cells, 2.71\u2009\u00b1\u20091.18\u2009\u00d7\u200910\n                      <jats:sup>6<\/jats:sup>\n                      pericytes and 1.88\u2009\u00b1\u20090.64\u2009\u00d7\u200910\n                      <jats:sup>6<\/jats:sup>\n                      endothelial cells. The polymerase chain reaction analysis revealed the following average values: Transforming growth factor beta 7.94\u2009\u00b1\u20093.31; vascular endothelial growth factor 12.43\u2009\u00b1\u20095.20; interleukin\u201010 7.54\u2009\u00b1\u20092.88; octamer\u2010binding transcription factor 3\/4 2.65\u2009\u00b1\u20091.69; interleukin\u20101 beta 5.45\u2009\u00b1\u20093.27 and ki\u201067 6.01\u2009\u00b1\u20093.65.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>A modification of an existing mechanical SVF preparation technique was introduced. The technique was feasible, safe and yielded a substantial volume of SVF (2.5\u20135\u2009mL). The SVF obtained had a high cellular composition. Age, gender and body mass index (BMI) did not affect the cell count, but elder patients presented a decreased composition in cytokines and growth factors.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Level of Evidence<\/jats:title>\n                    <jats:p>Level V.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jeo2.70378","type":"journal-article","created":{"date-parts":[[2025,7,27]],"date-time":"2025-07-27T12:09:21Z","timestamp":1753618161000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["A modified technique for mechanical isolation of stromal vascular fraction yields increased final product volume and high viable nucleated cells count"],"prefix":"10.1002","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5729-7755","authenticated-orcid":false,"given":"Trifon","family":"Totlis","sequence":"first","affiliation":[{"name":"Thessaloniki Minimally Invasive (The\u2010MIS) Orthopaedic Center, St. Luke's Hospital Thessaloniki Greece"},{"name":"School of Medicine, Faculty of Health Sciences Aristotle University of Thessaloniki Thessaloniki Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-2630-2552","authenticated-orcid":false,"given":"Panagiotis\u2010Konstantinos","family":"Emfietzis","sequence":"additional","affiliation":[{"name":"School of Medicine, Faculty of Health Sciences Aristotle University of Thessaloniki Thessaloniki Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Argiro","family":"Niti","sequence":"additional","affiliation":[{"name":"Biohellenika Biotechnology Company Thessaloniki Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vlasi\u03bfs","family":"Achlatis","sequence":"additional","affiliation":[{"name":"School of Medicine, Faculty of Health Sciences Aristotle University of Thessaloniki Thessaloniki Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5152-9532","authenticated-orcid":false,"given":"Lucienne A.","family":"Vonk","sequence":"additional","affiliation":[{"name":"Xintela AB Lund Sweden"},{"name":"Department of Orthopaedics University Medical Center Utrecht, Utrecht University Utrecht the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ioannis","family":"Terzidis","sequence":"additional","affiliation":[{"name":"Thessaloniki Minimally Invasive (The\u2010MIS) Orthopaedic Center, St. Luke's Hospital Thessaloniki Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kokkona","family":"Kouzi\u2010Koliakou","sequence":"additional","affiliation":[{"name":"Biohellenika Biotechnology Company Thessaloniki Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,7,27]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells10061365"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/jocd.12458"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1080\/14712598.2019.1671970"},{"key":"e_1_2_12_5_1","unstructured":"Arthrex GmbH. 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