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We investigated the prevalence, serotypes, and antimicrobial resistance genes (ARGs) of <jats:italic>Salmonella<\/jats:italic> in urban karst groundwater and groundwater\u2010fed surface waters. Weekly water samples (<jats:italic>n<\/jats:italic>\u00a0=\u00a0443) were collected from nine groundwater sites and the Barren River in Bowling Green, KY. Additionally, cross\u2010sectional samples were collected from 45 sites, including groundwaters and two rivers, in Tampa, FL. <jats:italic>Salmonella<\/jats:italic> was detected in 14.5% of Bowling Green samples resulting in 176 isolates representing 18 serotypes. Two (4.4%) of the Tampa samples were positive yielding eight isolates representing two serotypes. In Bowling Green, <jats:italic>Salmonella<\/jats:italic> prevalence varied by sampling site, week, month, and season, with the highest prevalence in surface water in the month of November and during summer. <jats:italic>Salmonella<\/jats:italic> Newport was the most common serotype detected. Nine of the serotypes detected in the study were among the top 20 serotypes commonly associated with human infections in the United States. Isolates were clustered with human clinical isolates, or isolates obtained from food animals, suggesting the importance of humans and agricultural practices as a source of water contamination. ARGs were detected in 12.5% (<jats:italic>n<\/jats:italic>\u00a0=\u00a0176) of the Bowling Green isolates; overall, 81.8% (<jats:italic>n<\/jats:italic>\u00a0=\u00a022) of these were multidrug\u2010resistant (resistance to \u22653 antimicrobial classes). Notably, the four <jats:italic>Salmonella<\/jats:italic> Agona isolates were resistant to four antimicrobial classes, mercury, and quaternary ammonium compounds. Seasonality and the widespread occurrence of <jats:italic>Salmonella<\/jats:italic> both in the groundwater and groundwater\u2010fed surface waters is a potential risk to public health.<\/jats:p>","DOI":"10.1002\/jeq2.20470","type":"journal-article","created":{"date-parts":[[2023,2,28]],"date-time":"2023-02-28T05:38:32Z","timestamp":1677562712000},"page":"691-705","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Prevalence and whole\u2010genome sequencing characterization of <i>Salmonella<\/i> in urban karst groundwater and predominantly groundwater\u2010fed surface waters for serotypes and antimicrobial resistance"],"prefix":"10.1002","volume":"52","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9903-5283","authenticated-orcid":false,"given":"Getahun E.","family":"Agga","sequence":"first","affiliation":[{"name":"USDA\u2010ARS, Food Animal Environmental Systems Research Unit  Bowling Green KY USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9020-4327","authenticated-orcid":false,"given":"Rachel","family":"Kaiser","sequence":"additional","affiliation":[{"name":"Department of Earth, Environmental, and Atmospheric Sciences Western Kentucky University  Bowling Green KY USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5997-7543","authenticated-orcid":false,"given":"Jason","family":"Polk","sequence":"additional","affiliation":[{"name":"Department of Earth, Environmental, and Atmospheric Sciences Western Kentucky University  Bowling Green KY USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marc","family":"Allard","sequence":"additional","affiliation":[{"name":"U.S. Food and Drug Administration Center for Food Safety and Applied Nutrition  College Park MD USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,3,24]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0132586"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1089\/fpd.2021.0037"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.watres.2019.115360"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2015.00415"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1097\/QCO.0b013e328358afe5"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid2701.200316"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1128\/ecosalplus.ESP\u20100008\u20102020"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.est.1c06472"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1289\/EHP7815"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.envres.2018.09.012"},{"key":"e_1_2_11_12_1","unstructured":"CDC. 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