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Over the last decade, there has been a growing concern of antibiotic residues entering the environment via animal manure. Similar studies have focused on the occurrence and biological effects of antibiotics in land\u2010applied animal feedlots; however, limited research has been conducted on the occurrence and persistence of antibiotics in animal feedlots. Therefore, the objective of this study was to evaluate antibiotic persistence, fate, and transport in surface water runoff and feedlot sediment in feedlot pens with livestock either receiving or not receiving antibiotic treatments through injection and feed. The two antibiotics (tylosin and monensin) added to animal feed were observed to persist in the soil environment for more than 30\u00a0days along with injected florfenicol. Monensin (5.6\u00d7 higher) and tylosin (20\u00d7 higher) were significantly higher in livestock pens receiving antibiotics compared to livestock pens not receiving the antibiotics. Further, rainfall was observed to significantly impact soil surface concentrations of florfenicol. Other antibiotics administrated by injection were not observed to statistically increase in concentrations in runoff or feedlot sediment. Our findings emphasize antibiotics administered in feedlots have the potential to persist and remain in feedlot sediment and runoff, particularly in instances of regular administration in feed.<\/jats:p>","DOI":"10.1002\/jeq2.20516","type":"journal-article","created":{"date-parts":[[2023,9,22]],"date-time":"2023-09-22T20:38:47Z","timestamp":1695415127000},"page":"1193-1205","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Occurrence and persistence of antibiotics administered to cattle in a newly established feedlot"],"prefix":"10.1002","volume":"52","author":[{"given":"Brittany","family":"Trejo","sequence":"first","affiliation":[{"name":"School of Natural Resources East Campus University of Nebraska\u2013Lincoln  Lincoln NE USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Matthew","family":"Russell","sequence":"additional","affiliation":[{"name":"Biosystems and Agricultural Engineering Department University of Kentucky  Lexington KY USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shannon","family":"Bartelt\u2010Hunt","sequence":"additional","affiliation":[{"name":"Department of Civil and Environmental Engineering University of Nebraska\u2013Lincoln  Lincoln NE USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nasrin Naderi","family":"Beni","sequence":"additional","affiliation":[{"name":"Department of Civil and Environmental Engineering University of Nebraska\u2013Lincoln  Lincoln NE USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0885-0504","authenticated-orcid":false,"given":"Daniel D.","family":"Snow","sequence":"additional","affiliation":[{"name":"Water Sciences Laboratory &amp; Nebraska Water Center, part of the Daugherty Water for Food Global Institute Institute of Agriculture and Natural Resources University of Nebraska\u2013Lincoln Lincoln Nebraska USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7772-9842","authenticated-orcid":false,"given":"Tiffany L.","family":"Messer","sequence":"additional","affiliation":[{"name":"Biosystems and Agricultural Engineering Department University of Kentucky  Lexington KY USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2023,10,11]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.2134\/jeq2015.07.0408"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.procbio.2006.03.010"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.envpol.2008.03.011"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jconhyd.2010.12.010"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1021\/es202680q"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chemosphere.2008.11.070"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.2134\/jeq2004.0261"},{"key":"e_1_2_10_9_1","unstructured":"Committee for Veterinary Medicinal Products (CVMP). 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