{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T16:41:27Z","timestamp":1790095287356,"version":"4.0.1"},"reference-count":32,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2024,4,4]],"date-time":"2024-04-04T00:00:00Z","timestamp":1712188800000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2024,4,4]],"date-time":"2024-04-04T00:00:00Z","timestamp":1712188800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100007917","name":"Agricultural Research Service","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100007917","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["acsess.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Env Quality"],"published-print":{"date-parts":[[2024,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Given the high cost and non\u2010renewability of mineral\u2010based fertilizers, there is increasing interest in the innovative use of manure\u2010based materials, such as poultry litter (PL). However, manure\u2010based fertilizers add both nutrients and microbes to the soil, including antibiotic\u2010resistant <jats:italic>Escherichia coli<\/jats:italic> (AREc). PL soil amendment impact on AREc in corn fields was evaluated in a randomized field experiment (May\u2013October 2017). Two winter cropping systems (fallow and cover crop) were assigned to whole plots, with three spring\u2010applied fertilizer treatments (untreated control [UC], PL, and commercial fertilizer [CF]) assigned to subplots. Soil was collected from 0 to 15\u00a0cm on days 0, 7, 28, 70, 98, and 172 post\u2010treatment applications. Samples were cultured for the enumeration and prevalence of generic, tetracycline\u2010resistant (TET<jats:sup>r<\/jats:sup>), third\u2010generation cephalosporin\u2010resistant (3GC<jats:sup>r<\/jats:sup>) <jats:italic>E. coli<\/jats:italic> isolates, and extended spectrum beta\u2010lactamase (ESBL)\u2010producing Enterobacteriaceae. PL soil amendment significantly (<jats:italic>p<\/jats:italic> &lt; 0.05) increased the levels of generic <jats:italic>E. coli<\/jats:italic>, TET<jats:sup>r<\/jats:sup> <jats:italic>E. coli<\/jats:italic>, and 3GC<jats:sup>r<\/jats:sup> <jats:italic>E. coli<\/jats:italic> on days 7 and 28 compared to UC or CF. Beyond day 28, AREc did not significantly (<jats:italic>p\u00a0<\/jats:italic>&gt;\u00a00.05) differ by fertilizer treatment and returned to baseline on day 70. ESBL\u2010producing Enterobacteriaceae were detected from 16 samples, mostly on day 70. Cover crop significantly decreased TET<jats:sup>r<\/jats:sup> <jats:italic>E. coli<\/jats:italic> concentration on day 28, with no significant effects on the prevalence of 3GC<jats:sup>r<\/jats:sup> <jats:italic>E. coli<\/jats:italic> and ESBL\u2010producing Enterobacteriaceae compared to no cover crop. All ESBL\u2010producing Enterobacteriaceae and 79% of the 3GC<jats:sup>r<\/jats:sup> <jats:italic>E. coli<\/jats:italic> isolates were positive for <jats:italic>bla<\/jats:italic><jats:sub>CTX\u2010M<\/jats:sub> gene by polymerase chain reaction. Results show that PL soil amendment transiently increases the levels of AREc compared to mineral fertilizer.<\/jats:p>","DOI":"10.1002\/jeq2.20560","type":"journal-article","created":{"date-parts":[[2024,4,5]],"date-time":"2024-04-05T02:50:11Z","timestamp":1712285411000},"page":"300-313","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Effect of poultry litter soil amendment on antibiotic\u2010resistant <i>Escherichia coli<\/i>"],"prefix":"10.1002","volume":"53","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9903-5283","authenticated-orcid":false,"given":"Getahun E.","family":"Agga","sequence":"first","affiliation":[{"name":"USDA\u2010ARS Food Animal Environmental Research Unit  Bowling Green Kentucky USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lisa M.","family":"Durso","sequence":"additional","affiliation":[{"name":"USDA\u2010ARS Agroecosystem Management Research Unit  Lincoln Nebraska USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karamat R.","family":"Sistani","sequence":"additional","affiliation":[{"name":"USDA\u2010ARS Food Animal Environmental Research Unit  Bowling Green Kentucky USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,4,4]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0132586"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0212510"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.3390\/antibiotics11030391"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jfp.2023.100144"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.4315\/JFP\u201022\u2010178"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/9781119192428.ch11"},{"key":"e_1_2_10_8_1","unstructured":"CDC. (2019).Antibiotic resistance threats in the United States 2019. Atlanta GA: U.S. Department of Health and Human Services U.S.Centers for Disease control and Prevention (CDC).http:\/\/www.cdc.gov\/drugresistance\/Biggest\u2010Threats.html"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.2134\/jeq2008.0128"},{"key":"e_1_2_10_10_1","volume-title":"Performance standards for antimicrobial susceptibility testing","author":"CLSI","year":"2023"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.2134\/jeq2013.10.0432"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mib.2011.04.003"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/9781119192428.ch12"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.apsoil.2020.103510"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365\u20102672.2008.03838.x"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.scitotenv.2020.139459"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijerph16193521"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jafc.3c05433"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1093\/femsec\/fiy010"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1139\/w2012\u2010082"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.2134\/jeq2019.02.0042"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0043933917000071"},{"key":"e_1_2_10_23_1","unstructured":"NASS. (2019).Poultry production and value: Final estimates 2013\u20132017(Statistical Bulletin Number 1061).United States Department of Agriculture National Agricultural Statistics Service."},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.2134\/asaspecpub67.c1"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.3389\/fenvs.2020.575466"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0160\u20104120(02)00084\u20103"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.02392\u201018"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.2134\/agronj2015.0529"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro1553"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.soilbio.2020.107965"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1409836111"},{"key":"e_1_2_10_32_1","volume-title":"Critically important antimicrobials for human medicine, 6th revision","author":"WHO","year":"2019"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2019.02639"}],"container-title":["Journal of Environmental Quality"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/acsess.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jeq2.20560","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/acsess.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jeq2.20560","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,16]],"date-time":"2025-10-16T16:34:37Z","timestamp":1760632477000},"score":1,"resource":{"primary":{"URL":"https:\/\/acsess.onlinelibrary.wiley.com\/doi\/10.1002\/jeq2.20560"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,4,4]]},"references-count":32,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2024,5]]}},"alternative-id":["10.1002\/jeq2.20560"],"URL":"https:\/\/doi.org\/10.1002\/jeq2.20560","archive":["Portico"],"relation":{},"ISSN":["0047-2425","1537-2537"],"issn-type":[{"value":"0047-2425","type":"print"},{"value":"1537-2537","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,4,4]]},"assertion":[{"value":"2023-12-27","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-03-14","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-04-04","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}