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Manure acidification and nitrification inhibitors (NIs) have been proposed as mitigation strategies, but their effectiveness likely varies with soil characteristics. To evaluate the influence of acidified and NI\u2010treated manure on N cycling across different soil types, we conducted a 28\u2010day laboratory incubation using soils collected from major agricultural regions in Wisconsin. We measured emissions of nitrous oxide (N\n                    <jats:sub>2<\/jats:sub>\n                    O), nitric oxide (NO), carbon dioxide (CO\n                    <jats:sub>2<\/jats:sub>\n                    ), and methane (CH\n                    <jats:sub>4<\/jats:sub>\n                    ), along with soil ammonium (NH\n                    <jats:sub>4<\/jats:sub>\n                    <jats:sup>+<\/jats:sup>\n                    ) and nitrate (NO\n                    <jats:sub>3<\/jats:sub>\n                    <jats:sup>\u2212<\/jats:sup>\n                    ) dynamics. Acidified manure enhanced short\u2010term NH\n                    <jats:sub>4<\/jats:sub>\n                    <jats:sup>+<\/jats:sup>\n                    retention in all soils but increased cumulative N\n                    <jats:sub>2<\/jats:sub>\n                    O emissions in the sandy soil compared to untreated manure, likely due to pH\u2010driven disruption of nitrification and incomplete denitrification. In contrast, the NI dicyandiamide (DCD) consistently suppressed nitrification early in the incubation, resulting in significantly lower N\n                    <jats:sub>2<\/jats:sub>\n                    O and NO emissions, often approaching levels observed in the no\u2010manure controls, particularly in the sandy soil. Manure amendments increased CO\n                    <jats:sub>2<\/jats:sub>\n                    fluxes relative to the no\u2010manure controls, but acidified manure emitted less CO\n                    <jats:sub>2<\/jats:sub>\n                    than DCD\u2010treated manure, likely due to temporary suppression of microbial respiration. CH\n                    <jats:sub>4<\/jats:sub>\n                    emissions were minimal and largely unaffected by treatments. NIs offer consistent benefits in reducing N\n                    <jats:sub>2<\/jats:sub>\n                    O and NO losses, while acidification can increase these emissions in certain soil conditions, highlighting the importance of tailoring management practices to specific soil characteristics.\n                  <\/jats:p>","DOI":"10.1002\/jeq2.70123","type":"journal-article","created":{"date-parts":[[2025,12,15]],"date-time":"2025-12-15T07:51:30Z","timestamp":1765785090000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Acidification and nitrification inhibition of manure alters greenhouse gas emissions and nitrogen cycling in diverse agricultural soils"],"prefix":"10.1002","volume":"55","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-0967-8471","authenticated-orcid":false,"given":"Mitch D.","family":"Wodrich","sequence":"first","affiliation":[{"name":"Department of Soil and Environmental Sciences University of Wisconsin\u2010Madison  Madison Wisconsin USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7841-2019","authenticated-orcid":false,"given":"Steven J.","family":"Hall","sequence":"additional","affiliation":[{"name":"Department of Plant and Agroecosystem Sciences University of Wisconsin\u2010Madison  Madison Wisconsin USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9050-3696","authenticated-orcid":false,"given":"Xia","family":"Zhu\u2010Barker","sequence":"additional","affiliation":[{"name":"Department of Soil and Environmental Sciences University of Wisconsin\u2010Madison  Madison Wisconsin USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,12,14]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0020453"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010017\u201001753\u20104"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1098\/rstb.2011.0321"},{"key":"e_1_2_10_5_1","volume-title":"Assessment of NOx emissions from soil in California cropping systems","author":"Burger M.","year":"2013"},{"key":"e_1_2_10_6_1","first-page":"207","article-title":"Influence of pH on the production of N2O and N2 by different denitrifying bacteria and Fusarium solani","volume":"35","author":"Burth I.","year":"1983","journal-title":"Ecological Bulletins"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1009747109538"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.agee.2014.09.008"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/sum.12194"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1080\/00288233.2013.797914"},{"key":"e_1_2_10_11_1","unstructured":"Code of Good Agricultural Practice (COGAP) for Reducing Ammonia Emissions. 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