{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T13:50:05Z","timestamp":1792158605169,"version":"4.4.0"},"reference-count":55,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["acsess.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Env Quality"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Native grasses of the Pampa biome are essential for ecosystem functioning and restoration, yet understanding of how native species respond to nutrient availability remains limited by challenges in standardizing plant material for controlled experiments. To address this, we developed a reproducible ex vitro propagation protocol to standardize native grass materials and applied it to three representative Pampa biome grasses,\n                    <jats:italic>Paspalum notatum<\/jats:italic>\n                    ,\n                    <jats:italic>Andropogon lateralis, and Axonopus affinis<\/jats:italic>\n                    . Using a minimally disturbed soil column system, we evaluated species\u2010specific responses to contrasting phosphorus (P) availability through measurements of plant growth, P dynamics, and phosphatase activity. Propagation was assessed using successive tiller transplants over 60 days, followed by a soil experiment to quantify plant growth, P dynamics, and phosphatase activity. The propagation protocol enabled rapid production of uniform plant material, generating up to 1197.9, 131.4, and 39.4 viable plantlets per initial plant for\n                    <jats:italic>A. affinis<\/jats:italic>\n                    ,\n                    <jats:italic>P. notatum<\/jats:italic>\n                    , and\n                    <jats:italic>A. lateralis<\/jats:italic>\n                    , respectively. Results indicate contrasting P\u2010use strategies among species.\n                    <jats:italic>Paspalum notatum<\/jats:italic>\n                    showed strong increases in biomass and leaf P concentration under P addition, consistent with a resource\u2010acquisitive strategy, and reduced phosphatase activity under high P availability. In contrast,\n                    <jats:italic>A. lateralis<\/jats:italic>\n                    exhibited limited growth response to P addition but maintained greater P\u2010use efficiency under low P conditions, indicative of a resource\u2010conservative strategy. These findings demonstrate that native grasses differ in their physiological responses to P availability, reflecting trade\u2010offs between productivity and nutrient\u2010use efficiency. The propagation protocol provides a standardized platform for controlled studies, while the identified functional differences support trait\u2010based species selection under contrasting soil P conditions.\n                  <\/jats:p>","DOI":"10.1002\/jeq2.70242","type":"journal-article","created":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T04:21:59Z","timestamp":1788322919000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Ex vitro standardization enables evaluation of phosphorus\u2010use strategies in Pampa biome grasses"],"prefix":"10.1002","volume":"55","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2268-5501","authenticated-orcid":false,"given":"J\u00falia","family":"Farias","sequence":"first","affiliation":[{"name":"USDA\u2010ARS, U.S. Arid Land Agricultural Research Center Maricopa Arizona USA"},{"name":"Departamento de Biologia, Centro de Ci\u00eancias Naturais e Exatas Universidade Federal de Santa Maria  Santa Maria Rio Grande do Sul Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0866-8761","authenticated-orcid":false,"given":"Anderson Cesar Ramos","family":"Marques","sequence":"additional","affiliation":[{"name":"Departamento de Biologia, Centro de Ci\u00eancias Naturais e Exatas Universidade Federal de Santa Maria  Santa Maria Rio Grande do Sul Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1969-5263","authenticated-orcid":false,"given":"Leandro Bittencourt","family":"de Oliveira","sequence":"additional","affiliation":[{"name":"Departamento de Fitotecnia e Fitossanidade, Setor de Ci\u00eancias Agr\u00e1rias Universidade Federal do Paran\u00e1  Curitiba Paran\u00e1 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bianca","family":"Knebel Del Frari","sequence":"additional","affiliation":[{"name":"Departamento de Biologia, Centro de 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