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However, the underlying mechanism responsible for the initiation of tumour cell dissemination and tumour metastasis in NPC is not well understood. Here, we demonstrated that epidermal growth factor receptor (EGFR) was highly expressed in tumour tissues of NPC patients with distant metastases and was associated with a decrease in reactive oxygen species (ROS). We also revealed that extracellular vesicles (EVs) transfer occurred from highly to poorly metastatic NPC cells, mediating cell\u2013cell communication and enhancing the metastatic potential of poorly metastatic NPC cells. Further experiments indicated that EVs derived from highly metastatic NPC cells induced the up\u2010regulation of EGFR and down\u2010regulation of ROS in low metastatic NPC cells. Mechanistically, EGFR\u2010rich EVs\u2010mediated EGFR overexpression down\u2010regulated intracellular ROS levels through the PI3K\/AKT pathway, thus promoting the metastatic potential of poorly metastatic NPC cells. Strikingly, treatment with EVs secreted from highly metastatic NPC cells was significantly associated with rapid NPC progression and shorter survival in xenografted mice. These findings not only improve our understanding of EVs\u2010mediated NPC metastatic mechanism but also have important implications for the detection and treatment of NPC patients accompanied by aberrant EGFR\u2010rich EVs transmission.<\/jats:p>","DOI":"10.1002\/jev2.12003","type":"journal-article","created":{"date-parts":[[2020,12,3]],"date-time":"2020-12-03T10:40:45Z","timestamp":1606992045000},"source":"Crossref","is-referenced-by-count":39,"title":["EGFR\u2010rich extracellular vesicles derived from highly metastatic nasopharyngeal carcinoma cells accelerate tumour metastasis through PI3K\/AKT pathway\u2010suppressed ROS"],"prefix":"10.1002","volume":"10","author":[{"given":"Fei","family":"Li","sequence":"first","affiliation":[{"name":"State Key Laboratory of Biocontrol School of Life Sciences Sun Yat\u2010sen University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin","family":"Zhao","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Biocontrol School of Life Sciences Sun Yat\u2010sen University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rui","family":"Sun","sequence":"additional","affiliation":[{"name":"Department of Nasopharyngeal Carcinoma State Key Laboratory of Oncology in South China Collaborative Innovation Center for Cancer Medicine Sun Yat\u2010sen University Cancer Center  Guangzhou China"},{"name":"Guangdong Key Laboratory of Nasopharyngeal Carcinoma Diagnosis and Therapy  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinxin","family":"Ou","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Biocontrol School of Life Sciences Sun Yat\u2010sen University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junyu","family":"Huang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Biocontrol School of Life Sciences Sun Yat\u2010sen University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nanyan","family":"Yang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Biocontrol School of Life Sciences Sun Yat\u2010sen University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ting","family":"Xu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Biocontrol School of Life Sciences Sun Yat\u2010sen University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingyao","family":"Li","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Biocontrol School of Life Sciences Sun Yat\u2010sen University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiner","family":"He","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Biocontrol School of Life Sciences Sun Yat\u2010sen University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chaoyi","family":"Li","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Biocontrol School of Life Sciences Sun Yat\u2010sen University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mo","family":"Yang","sequence":"additional","affiliation":[{"name":"The Seventh Affiliated Hospital Sun Yat\u2010sen University  Shenzhen China"},{"name":"Lianjiang People's Hospital  Lianjiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qing","family":"Zhang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Biocontrol School of Life Sciences Sun Yat\u2010sen University  Guangzhou China"},{"name":"Institute of Sun Yat\u2010sen University in Shenzhen  Shenzhen China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,10,30]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/cvr\/cvx115"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1080\/20013078.2019.1626654"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrc2981"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncr.28001"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.29562"},{"issue":"7","key":"e_1_2_8_7_1","first-page":"496","article-title":"Recent advances in the treatment of nasopharyngeal carcinoma","volume":"103","author":"Chang J. 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