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Building on our previous understanding of how syntenin, and its PDZ partner syndecan (SDC), impact on exosome composition we optimized a small chemical compound targeting the PDZ2 domain of syntenin. In vitro , in tests on MCF\u20107 breast carcinoma cells, this compound is non\u2010toxic and impairs cell proliferation, migration and primary sphere formation. It does not affect the size or the number of secreted particles, yet it decreases the amounts of exosomal syntenin, ALIX and SDC4 while leaving other exosomal markers unaffected. Interestingly, it also blocks the sorting of EpCAM, a <jats:italic>bona fide<\/jats:italic> target used for carcinoma exosome immunocapture. Our study highlights the first characterization of a small pharmacological inhibitor of the syntenin\u2010exosomal pathway, of potential interest for exosome research and oncology.<\/jats:p>","DOI":"10.1002\/jev2.12039","type":"journal-article","created":{"date-parts":[[2020,12,19]],"date-time":"2020-12-19T23:57:23Z","timestamp":1608422243000},"source":"Crossref","is-referenced-by-count":56,"title":["Pharmacological inhibition of syntenin PDZ2 domain impairs breast cancer cell activities and exosome loading with syndecan and EpCAM cargo"],"prefix":"10.1002","volume":"10","author":[{"given":"R.","family":"Leblanc","sequence":"first","affiliation":[{"name":"Equipe labellis\u00e9e Ligue 2018 Centre de Recherche en Canc\u00e9rologie de Marseille (CRCM) Aix\u2010Marseille Universit\u00e9, Inserm, CNRS, Institut Paoli\u2010Calmettes  Marseille France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.","family":"Kashyap","sequence":"additional","affiliation":[{"name":"Equipe labellis\u00e9e Ligue 2018 Centre de Recherche en Canc\u00e9rologie de Marseille (CRCM) Aix\u2010Marseille Universit\u00e9, Inserm, CNRS, Institut Paoli\u2010Calmettes  Marseille France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K.","family":"Barral","sequence":"additional","affiliation":[{"name":"Centre de Recherche en Canc\u00e9rologie de Marseille Integrative Structural &amp; Chemical Biology Aix\u2010Marseille Universit\u00e9, Inserm, CNRS, Institut Paoli Calmettes  Marseille France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.L.","family":"Egea\u2010Jimenez","sequence":"additional","affiliation":[{"name":"Equipe labellis\u00e9e Ligue 2018 Centre de Recherche en Canc\u00e9rologie de Marseille (CRCM) Aix\u2010Marseille Universit\u00e9, Inserm, CNRS, Institut Paoli\u2010Calmettes  Marseille France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D.","family":"Kovalskyy","sequence":"additional","affiliation":[{"name":"Enamine Ltd.  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