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However, little is known about the contribution of EVs derived from cancer cells to the vicious cycle of bone metastasis. Here, we report a direct regulatory mode between tumour cells and osteoclasts in metastatic niche of prostate cancer via vesicular miRNAs transfer. Combined analysis of miRNAs profiles both in tumour\u2010derived small EVs (sEVs) and osteoclasts identified miR\u2010152\u20103p as a potential osteolytic molecule. sEVs were enriched in miR\u2010152\u20103p, which targets osteoclastogenic regulator MAFB. Blocking miR\u2010152\u20103p in sEVs upregulated the expression of MAFB and impaired osteoclastogenesis in vitro. In vivo experiments of xenograft mouse model found that blocking of miR\u2010152\u20103p in sEVs significantly slowed down the loss of trabecular architecture, while systemic inhibition of miR\u2010152\u20103p using antagomir\u2010152\u20103p reduced the osteolytic lesions of cortical bone while preserving basic trabecular architecture. Our findings suggest that miR\u2010152\u20103p carried by prostate cancer\u2010derived sEVs deliver osteolytic signals from tumour cells to osteoclasts, facilitating osteolytic progression in bone metastasis.<\/jats:p>","DOI":"10.1002\/jev2.12068","type":"journal-article","created":{"date-parts":[[2021,2,20]],"date-time":"2021-02-20T17:09:22Z","timestamp":1613840962000},"source":"Crossref","is-referenced-by-count":45,"title":["Small extracellular vesicles deliver osteolytic effectors and mediate cancer\u2010induced osteolysis in bone metastatic niche"],"prefix":"10.1002","volume":"10","author":[{"given":"Qinyu","family":"Ma","sequence":"first","affiliation":[{"name":"Department of Orthopedics Southwest Hospital Third Military Medical University  Chongqing 400038 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mengmeng","family":"Liang","sequence":"additional","affiliation":[{"name":"Department of Biomedical Materials Science Third Military Medical University  Chongqing 400038 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yutong","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Orthopedics Southwest Hospital Third Military Medical University  Chongqing 400038 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ce","family":"Dou","sequence":"additional","affiliation":[{"name":"Department of Orthopedics Southwest Hospital Third Military Medical University  Chongqing 400038 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianzhong","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Orthopedics Southwest Hospital Third Military Medical University  Chongqing 400038 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shiwu","family":"Dong","sequence":"additional","affiliation":[{"name":"Department of Biomedical Materials Science Third Military Medical University  Chongqing 400038 China"},{"name":"State Key Laboratory of Trauma Burns and Combined Injury Third Military Medical University  Chongqing 400038 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fei","family":"Luo","sequence":"additional","affiliation":[{"name":"Department of Orthopedics Southwest Hospital Third Military Medical University  Chongqing 400038 China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,2,18]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12079-015-0311-9"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature02871"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(04)00045-5"},{"issue":"2","key":"e_1_2_8_5_1","first-page":"114","article-title":"How close are miRNAs from clinical practice? 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