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Elevation of their plasmatic level has been reported in a variety of infectious and cardiovascular disorders, but the clinical use of this potential biomarker is hampered by methodological issues. Although flow cytometry (FCM) is currently used to detect NDEVs in the plasma of patients, an extensive characterization of NDEVs has never been done. Moreover, their detection remains challenging because of their small size and low antigen density. Therefore, the objective of the present study was first to establish a surface antigenic signature of NDEVs detectable by FCM and therefore to improve their detection in biological fluids by developing a strategy allowing to overcome their low fluorescent signal and reduce the background noise. By testing a large panel of 54 antibody specificities already reported to be positive on PMNs, we identified a profile of 15 membrane protein markers, including 4 (CD157, CD24, CD65 and CD66c) never described on NDEVs. Among them, CD15, CD66b and CD66c were identified as the most sensitive and specific markers to detect NDEVs by FCM. Using this antigenic signature, we developed a new strategy combining the three best antibodies in a cocktail and reducing the background noise by size exclusion chromatography (SEC). This strategy allowed a significant improvement in NDEVs enumeration in plasma from sepsis patients and made it feasible to efficiently sort NDEVs from COVID\u201019 patients. Altogether, this work opens the door to a more valuable measurement of NDEVs as a potential biomarker in clinical practice. A similar strategy could also be applied to improve detection by FCM of other rare subpopulations of EVs generated by tissues with limited access, such as vascular endothelium, cancer cells or placenta.<\/jats:p>","DOI":"10.1002\/jev2.12204","type":"journal-article","created":{"date-parts":[[2022,4,1]],"date-time":"2022-04-01T00:23:55Z","timestamp":1648772635000},"source":"Crossref","is-referenced-by-count":16,"title":["A new strategy to count and sort neutrophil\u2010derived extracellular vesicles: Validation in infectious disorders"],"prefix":"10.1002","volume":"11","author":[{"given":"Amandine","family":"Bonifay","sequence":"first","affiliation":[{"name":"Aix\u2010Marseille University, C2VN, INSERM 1263, INRA 1260  Marseille France"},{"name":"Department of Hematology and Vascular Biology CHU La Conception, APHM  Marseille 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