{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T18:21:33Z","timestamp":1790878893092,"version":"4.1.0"},"reference-count":37,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2022,5,18]],"date-time":"2022-05-18T00:00:00Z","timestamp":1652832000000},"content-version":"vor","delay-in-days":17,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Extracellular Vesicle"],"published-print":{"date-parts":[[2022,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Amniotic fluid surrounding the developing fetus is a complex biological fluid rich in metabolically active bio\u2010factors. The presence of extracellular vesicles (EVs) in amniotic fluid has been mainly related to foetal urine. We here characterized EVs from term amniotic fluid in terms of surface marker expression using different orthogonal techniques. EVs appeared to be a heterogeneous population expressing markers of renal, placental, epithelial and stem cells. Moreover, we compared amniotic fluid EVs from normal pregnancies with those of preeclampsia, a hypertensive disorder affecting up to 8% of pregnancies worldwide. An increase of CD105 (endoglin) expressing EVs was observed in preeclamptic amniotic fluid by bead\u2010based cytofluorimetric analysis, and further confirmed using a chip\u2010based analysis. HLA\u2010G, a typical placental marker, was not co\u2010expressed by the majority of CD105\n                    <jats:sup>+<\/jats:sup>\n                    EVs, in analogy with amniotic fluid stromal cell derived\u2010EVs. At a functional level, preeclampsia\u2010derived EVs, but not normal pregnancy EVs, showed an antiangiogenic effect, possibly due to the decoy effect of endoglin. Our results provide a characterization of term amniotic fluid\u2010EVs, supporting their origin from foetal and placental cells. In preeclampsia, the observed antiangiogenic characteristics of amniotic fluid\u2010EVs may reflect the hypoxic and antiangiogenic microenvironment and could possibly impact on the developing fetus or on the surrounding foetal membranes.\n                  <\/jats:p>","DOI":"10.1002\/jev2.12217","type":"journal-article","created":{"date-parts":[[2022,5,18]],"date-time":"2022-05-18T03:59:44Z","timestamp":1652846384000},"source":"Crossref","is-referenced-by-count":46,"title":["Single extracellular vesicle analysis in human amniotic fluid shows evidence of phenotype alterations in preeclampsia"],"prefix":"10.1002","volume":"11","author":[{"given":"Natalia","family":"Gebara","sequence":"first","affiliation":[{"name":"Department of Molecular Biotechnology and Health Sciences University of Turin  Turin Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julia","family":"Scheel","sequence":"additional","affiliation":[{"name":"Department of Systems Biology and Bioinformatics University of Rostock  Rostock Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Renata","family":"Skovronova","sequence":"additional","affiliation":[{"name":"Department of Molecular Biotechnology and Health Sciences University of Turin  Turin Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cristina","family":"Grange","sequence":"additional","affiliation":[{"name":"Department of Medical Sciences University of Turin  Turin Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luca","family":"Marozio","sequence":"additional","affiliation":[{"name":"Department of Surgical Sciences, Obstetrics and Gynecology, University of Turin  Turin Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shailendra","family":"Gupta","sequence":"additional","affiliation":[{"name":"Department of Systems Biology and Bioinformatics University of Rostock  Rostock Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Veronica","family":"Giorgione","sequence":"additional","affiliation":[{"name":"Vascular Biology Research Centre Molecular and Clinical Sciences Research Institute St George's University of London  London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Federico","family":"Caicci","sequence":"additional","affiliation":[{"name":"DiBio Imaging Facility University of Padua  Padua Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chiara","family":"Benedetto","sequence":"additional","affiliation":[{"name":"Department of Surgical Sciences, Obstetrics and Gynecology, University of Turin  Turin Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Asma","family":"Khalil","sequence":"additional","affiliation":[{"name":"Vascular Biology Research Centre Molecular and Clinical Sciences Research Institute St George's University of London  London UK"},{"name":"Foetal Medicine Unit St George's University Hospitals NHS Foundation Trust St George's University of London  London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3663-5134","authenticated-orcid":false,"given":"Benedetta","family":"Bussolati","sequence":"additional","affiliation":[{"name":"Department of Molecular Biotechnology and Health Sciences University of Turin  Turin Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,5,18]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.2147\/VHRM.S64798"},{"issue":"3","key":"e_1_2_7_3_1","first-page":"208","article-title":"Isolation, differentiation, and characterization of mesenchymal stem cells from human bone marrow","volume":"10","author":"Baghaei K.","year":"2017","journal-title":"Gastroenterology and Hepatology from Bed to Bench"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/sctm.16-0297"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.25206"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1161\/HYPERTENSIONAHA.117.10803"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1472-6483(10)60111-3"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1161\/HYPERTENSIONAHA.118.11706"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0104490"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.2018-00073"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.semperi.2009.02.010"},{"key":"e_1_2_7_12_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3389\/fphys.2016.00098","article-title":"Role of extracellular vesicles and microRNAs on dysfunctional angiogenesis during preeclamptic pregnancies","volume":"7","author":"Escudero C. 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