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In this study, we compared mesenchymal stem cell (MSC)\u2010derived apoVs and exosomes from three different types of MSCs including human bone marrow MSCs (hBMSCs), human adipose MSCs (hASCs), and mouse bone marrow MSCs (mBMSCs). We established a unique protein map of MSC\u2010derived apoVs and identified the differences between apoVs and exosomes in terms of functional protein cargo and surface markers. Furthermore, we identified 13 proteins specifically enriched in apoVs compared to exosomes, which can be used as apoV\u2010specific biomarkers. In addition, we showed that apoVs inherited apoptotic imprints such as Fas to ameliorate haemophilia A in factor VIII knockout mice via binding to the platelets\u2019 FasL to activate platelet functions, and therefore rescuing the blood clotting disorder. In summary, we systemically characterized MSC\u2010derived apoVs and identified their therapeutic role in haemophilia A treatment through a previously unknown Fas\/FasL linkage mechanism.<\/jats:p>","DOI":"10.1002\/jev2.12240","type":"journal-article","created":{"date-parts":[[2022,7,2]],"date-time":"2022-07-02T00:14:18Z","timestamp":1656720858000},"source":"Crossref","is-referenced-by-count":104,"title":["Proteomic analysis of MSC\u2010derived apoptotic vesicles identifies Fas inheritance to ameliorate haemophilia a via activating platelet functions"],"prefix":"10.1002","volume":"11","author":[{"given":"Xiao","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Prosthodontics Peking University School and Hospital of Stomatology &amp; National Center of Stomatology &amp; National Clinical Research Center for Oral Diseases &amp; National Engineering Laboratory for Digital and Material Technology of Stomatology &amp; Beijing Key Laboratory of Digital Stomatology 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