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Here, we tagged CD63\u2010 or CD9\u2010positive EVs secreted by triple negative breast cancer cells with Nanoluciferase enzyme, to set\u2010up a miniaturized method to quantify secretion of these two EV subtypes directly in the supernatant of cells. We performed a cell\u2010based high\u2010content screening to identify clinically\u2010approved drugs able to affect EV secretion. One of the identified hits is Homosalate, an anti\u2010inflammatory drug found in sunscreens which robustly increased EVs\u2019 release. Comparing EVs induced by Homosalate with those induced by Bafilomycin A1, we demonstrate that: (1) the two drugs act on EVs generated in distinct subcellular compartments, and (2) EVs released by Homosalate\u2010, but not by Bafilomycin A1\u2010treated cells enhance resistance to anchorage loss in another recipient epithelial tumour cell line. In conclusion, we identified a new drug modifying EV release and demonstrated that under influence of different drugs, triple negative breast cancer cells release EV subpopulations from different subcellular origins harbouring distinct functional properties.<\/jats:p>","DOI":"10.1002\/jev2.12242","type":"journal-article","created":{"date-parts":[[2022,7,5]],"date-time":"2022-07-05T03:44:57Z","timestamp":1656992697000},"source":"Crossref","is-referenced-by-count":19,"title":["Homosalate boosts the release of tumour\u2010derived extracellular vesicles with protection against anchorage\u2010loss property"],"prefix":"10.1002","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4585-9244","authenticated-orcid":false,"given":"Eleonora","family":"Grisard","sequence":"first","affiliation":[{"name":"Institut Curie PSL Research University  INSERM U932 Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6350-7122","authenticated-orcid":false,"given":"Nathalie","family":"Nevo","sequence":"additional","affiliation":[{"name":"Institut Curie PSL Research University  INSERM U932 Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aurianne","family":"Lescure","sequence":"additional","affiliation":[{"name":"Institut Curie PSL Research University, Translational Research Department, BioPhenics Platform  PICT\u2010IBISA Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8933-5362","authenticated-orcid":false,"given":"Sebastian","family":"Doll","sequence":"additional","affiliation":[{"name":"Institute of Metabolism and Cell Death Helmholtz Zentrum M\u00fcnchen  Neuherberg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maxime","family":"Corb\u00e9","sequence":"additional","affiliation":[{"name":"Institut Curie PSL Research University, Translational Research Department, BioPhenics Platform  PICT\u2010IBISA Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3115-4681","authenticated-orcid":false,"given":"Mabel","family":"Jouve","sequence":"additional","affiliation":[{"name":"Institut Curie PSL Research University  CNRS UMR3215 Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9852-4977","authenticated-orcid":false,"given":"Gregory","family":"Lavieu","sequence":"additional","affiliation":[{"name":"Institut Curie PSL Research University  INSERM U932 Paris France"},{"name":"Universit\u00e9 Paris Cit\u00e9, INSERM U1316  CNRS UMR 7057 Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2215-1550","authenticated-orcid":false,"given":"Alain","family":"Joliot","sequence":"additional","affiliation":[{"name":"Institut Curie PSL Research University  INSERM U932 Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9654-5202","authenticated-orcid":false,"given":"Elaine Del","family":"Nery","sequence":"additional","affiliation":[{"name":"Institut Curie PSL Research University, Translational Research Department, BioPhenics Platform  PICT\u2010IBISA Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1511-8576","authenticated-orcid":false,"given":"Lorena","family":"Martin\u2010Jaular","sequence":"additional","affiliation":[{"name":"Institut Curie PSL Research University  INSERM U932 Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8294-6884","authenticated-orcid":false,"given":"Clotilde","family":"Th\u00e9ry","sequence":"additional","affiliation":[{"name":"Institut Curie PSL Research University  INSERM U932 Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,7,5]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/jat.3525"},{"key":"e_1_2_8_3_1","first-page":"1","article-title":"Interferences with Luciferase Reporter Enzymes","author":"Auld D. 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