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While recent studies on EV\u2010associated DNA (EV\u2010DNA) in human biofluids have generated a large amount of data, there is currently no database that catalogues information on EV\u2010DNA. To fill this gap, we have manually curated a database of EV\u2010DNA data derived from human biofluids (liquid biopsy) and <jats:italic>in\u2010vitro<\/jats:italic> studies, called the <jats:styled-content>E<\/jats:styled-content>xtracellular <jats:styled-content>V<\/jats:styled-content>esicle\u2010<jats:styled-content>A<\/jats:styled-content>ssociated <jats:styled-content>D<\/jats:styled-content>NA <jats:styled-content>D<\/jats:styled-content>atabase (EV\u2010ADD). This database contains validated experimental details and data extracted from peer\u2010reviewed published literature. It can be easily queried to search for EV isolation methods and characterization, EV\u2010DNA isolation techniques, quality validation, DNA fragment size, volume of starting material, gene names and disease context. Currently, our database contains samples representing 23 diseases, with 13 different types of EV isolation techniques applied on eight different human biofluids (e.g. blood, saliva). In addition, EV\u2010ADD encompasses EV\u2010DNA data both representing the whole genome and specifically including oncogenes, such as <jats:italic>KRAS, EGFR, BRAF, MYC<\/jats:italic>, and mitochondrial DNA (mtDNA). An EV\u2010ADD data metric system was also integrated to assign a compliancy score to the MISEV guidelines based on experimental parameters reported in each study. While currently available databases document the presence of proteins, lipids, RNA and metabolites in EVs (e.g. Vesiclepedia, ExoCarta, ExoBCD, EVpedia, and EV\u2010TRACK), to the best of our knowledge, EV\u2010ADD is the first of its kind to compile all available EV\u2010DNA datasets derived from human biofluid samples. We believe that this database provides an important reference resource on EV\u2010DNA\u2010based liquid biopsy research, serving as a learning tool and to showcase the latest developments in the EV\u2010DNA field. EV\u2010ADD will be updated yearly as newly published EV\u2010DNA data becomes available and it is freely available at <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/www.evdnadatabase.com\">www.evdnadatabase.com<\/jats:ext-link>.<\/jats:p>","DOI":"10.1002\/jev2.12270","type":"journal-article","created":{"date-parts":[[2022,10,22]],"date-time":"2022-10-22T09:34:05Z","timestamp":1666431245000},"source":"Crossref","is-referenced-by-count":39,"title":["EV\u2010ADD, a database for EV\u2010associated DNA in human liquid biopsy samples"],"prefix":"10.1002","volume":"11","author":[{"given":"Thupten","family":"Tsering","sequence":"first","affiliation":[{"name":"Cancer Research Program Research Institute of the McGill University Health Centre  Montreal Quebec Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mingyang","family":"Li","sequence":"additional","affiliation":[{"name":"Cancer Research Program Research Institute of the McGill 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