{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,18]],"date-time":"2026-10-18T03:06:39Z","timestamp":1792292799902,"version":"4.4.0"},"reference-count":101,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2022,11,2]],"date-time":"2022-11-02T00:00:00Z","timestamp":1667347200000},"content-version":"vor","delay-in-days":1,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Extracellular Vesicle"],"published-print":{"date-parts":[[2022,11]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    The choroid plexus secrets cerebrospinal fluid (CSF) composed of electrolytes, cytokines, growth factors, metabolites and extracellular vesicles (EVs) that flow through the interconnected brain ventricles. On their course, CSF components can act as signals that affect, for example, neural stem cells (NSCs) residing in niches of the ventricular wall. We studied EV\u2010born CSF signals in an in vitro culture system. We purified EVs from the secretome of a choroid plexus cell line (Z310 cells), and from primary choroid plexus cultures and co\u2010cultured those EVs with NSCs isolated from the niche of the lateral and the third ventricle. EVs\n                    <jats:sup>Z310<\/jats:sup>\n                    and EVs\n                    <jats:sup>CHP<\/jats:sup>\n                    were purified by differential centrifugation. This yielded fractions of EVs of 50\u2013150\u2010nm diameter that induced a complex multicellular network formation and NSC differentiation. Both types of EV converted the round NSCs to cells that extended long processes that contacted nearby, alike\u2010shaped cells. Mass spectrometry showed that the differentiation\u2010inducing EV\n                    <jats:sup>Z310<\/jats:sup>\n                    were enriched for membrane and membrane\u2010associated proteins involved in cell differentiation, membrane trafficking, and membrane organization. We hypothesize that this type of EV\n                    <jats:sup>Z310<\/jats:sup>\n                    cargo causes changes of stem cell morphology that leads to multicellular networks in the niches. This cell\u2010shape transition may represent an initial step in NSC differentiation.\n                  <\/jats:p>","DOI":"10.1002\/jev2.12276","type":"journal-article","created":{"date-parts":[[2022,11,3]],"date-time":"2022-11-03T02:36:48Z","timestamp":1667443008000},"source":"Crossref","is-referenced-by-count":12,"title":["Extracellular vesicles derived from the choroid plexus trigger the differentiation of neural stem cells"],"prefix":"10.1002","volume":"11","author":[{"given":"Zuzana","family":"Ditte","sequence":"first","affiliation":[{"name":"Department of Genes and Behavior Max Planck Institute for Multidisciplinary Sciences  G\u00f6ttingen Germany"},{"name":"Biological Rhythms Max Planck Institute for Dynamics and Self Organization  G\u00f6ttingen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ivan","family":"Silbern","sequence":"additional","affiliation":[{"name":"The Bioanalytical Mass Spectrometry Group Max Planck Institute for Multidisciplinary Sciences  G\u00f6ttingen Germany"},{"name":"Institute for Clinical Chemistry University Medical Center G\u00f6ttingen  G\u00f6ttingen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Ditte","sequence":"additional","affiliation":[{"name":"Department of Genes and Behavior Max Planck Institute for Multidisciplinary Sciences  G\u00f6ttingen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Henning","family":"Urlaub","sequence":"additional","affiliation":[{"name":"The Bioanalytical Mass Spectrometry Group Max Planck Institute for Multidisciplinary Sciences  G\u00f6ttingen Germany"},{"name":"Institute for Clinical Chemistry University Medical Center G\u00f6ttingen  G\u00f6ttingen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gregor","family":"Eichele","sequence":"additional","affiliation":[{"name":"Department of Genes and Behavior Max Planck Institute for Multidisciplinary Sciences  G\u00f6ttingen Germany"},{"name":"Biological Rhythms Max Planck Institute for Dynamics and Self Organization  G\u00f6ttingen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,11,2]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-physiol-020911-153305"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.15252\/emmm.201606271"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/adhm.202002048"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gky1049"},{"key":"e_1_2_9_6_1","first-page":"501","article-title":"Exosomes as novel regulators of adult neurogenic niches","volume":"9","author":"Batiz L. F.","year":"2015","journal-title":"Frontiers in Cellular Neuroscience"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-021-22126-y"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1177\/1759091420981206"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2018.06.004"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2015.11.015"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1021\/pr101065j"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.7770779"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1080\/20013078.2020.1722433"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.1971.51.2.273"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1152\/physiol.00011.2010"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00401-009-0624-y"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1093\/braincomms\/fcaa150"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/35096019"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)80783-7"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41593-020-00770-9"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41583-021-00525-w"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1098\/rstb.2019.0154"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2020.01.027"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aae0450"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0088810"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.1001604"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00401-018-1807-1"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms3123"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.2437-12.2013"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-08-3176"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2016.12.001"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1186\/2045-8118-11-26"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41594-019-0263-5"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00441-019-03132-5"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.15252\/msb.20145625"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.E17-05-0271"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)48095-7"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-019-09298-4"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.1001450"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.aav0618"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2015.09.019"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1186\/1743-8454-7-11"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.stem.2012.06.016"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1155\/2018\/8545347"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1521230113"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.23355"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2011.01.023"},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.15252\/embj.2018100481"},{"key":"e_1_2_9_49_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells8101118"},{"key":"e_1_2_9_50_1","doi-asserted-by":"publisher","DOI":"10.1002\/glia.22558"},{"key":"e_1_2_9_51_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21228819"},{"key":"e_1_2_9_52_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2012.11.024"},{"key":"e_1_2_9_53_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrn3921"},{"key":"e_1_2_9_54_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.3000363"},{"key":"e_1_2_9_55_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41556-018-0250-9"},{"key":"e_1_2_9_56_1","doi-asserted-by":"publisher","DOI":"10.5772\/61186"},{"key":"e_1_2_9_57_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-019-11534-w"},{"key":"e_1_2_9_58_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-59745-019-5_15"},{"key":"e_1_2_9_59_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.3744-09.2010"},{"key":"e_1_2_9_60_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M112.410944"},{"key":"e_1_2_9_61_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2019.02.018"},{"key":"e_1_2_9_62_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-018-03507-2"},{"key":"e_1_2_9_63_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2016.12.158"},{"key":"e_1_2_9_64_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0928-4257(01)00084-5"},{"key":"e_1_2_9_65_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.156059"},{"key":"e_1_2_9_66_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.24376"},{"key":"e_1_2_9_67_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gky1106"},{"key":"e_1_2_9_68_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms3049"},{"key":"e_1_2_9_69_1","doi-asserted-by":"publisher","DOI":"10.1080\/20013078.2019.1684862"},{"key":"e_1_2_9_70_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1119133"},{"key":"e_1_2_9_71_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature10098"},{"key":"e_1_2_9_72_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.20288"},{"key":"e_1_2_9_73_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0234614"},{"key":"e_1_2_9_74_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1902513116"},{"key":"e_1_2_9_75_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tiv.2007.07.007"},{"key":"e_1_2_9_76_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-cancerbio-030617-050519"},{"key":"e_1_2_9_77_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1712108114"},{"key":"e_1_2_9_78_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-0716-1024-4_14"},{"key":"e_1_2_9_79_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.stem.2016.06.013"},{"key":"e_1_2_9_80_1","doi-asserted-by":"publisher","DOI":"10.1007\/0-387-29362-0_23"},{"key":"e_1_2_9_81_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.202101075"},{"key":"e_1_2_9_82_1","doi-asserted-by":"publisher","DOI":"10.1111\/1467-9868.00346"},{"key":"e_1_2_9_83_1","doi-asserted-by":"publisher","DOI":"10.1186\/1479-5876-10-5"},{"key":"e_1_2_9_84_1","doi-asserted-by":"publisher","DOI":"10.1186\/1743-8454-4-11"},{"key":"e_1_2_9_85_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2006.10.030"},{"key":"e_1_2_9_86_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2011870117"},{"key":"e_1_2_9_87_1","doi-asserted-by":"publisher","DOI":"10.1080\/20013078.2018.1535750"},{"key":"e_1_2_9_88_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0113116"},{"key":"e_1_2_9_89_1","doi-asserted-by":"publisher","DOI":"10.1098\/rstb.2016.0479"},{"key":"e_1_2_9_90_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncb1596"},{"key":"e_1_2_9_91_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm.2017.125"},{"key":"e_1_2_9_92_1","doi-asserted-by":"publisher","DOI":"10.1093\/jb\/mvj128"},{"key":"e_1_2_9_93_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetimm.2008.04.002"},{"issue":"84","key":"e_1_2_9_94_1","first-page":"e51225","article-title":"One mouse, two cultures: Isolation and culture of adult neural stem cells from the two neurogenic zones of individual mice","volume":"2014","author":"Walker T. L.","year":"2014","journal-title":"Journal of Visualized Experiments: JoVE"},{"key":"e_1_2_9_95_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.expneurol.2022.114124"},{"key":"e_1_2_9_96_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1207003"},{"key":"e_1_2_9_97_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neulet.2016.10.042"},{"key":"e_1_2_9_98_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pneurobio.2018.04.001"},{"key":"e_1_2_9_99_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-012-0957-x"},{"key":"e_1_2_9_100_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature23282"},{"key":"e_1_2_9_101_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-8993(02)03683-1"},{"key":"e_1_2_9_102_1","doi-asserted-by":"publisher","DOI":"10.1016\/bs.ctdb.2020.02.005"}],"container-title":["Journal of Extracellular Vesicles"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jev2.12276","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jev2.12276","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/isevjournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jev2.12276","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,16]],"date-time":"2025-10-16T13:38:28Z","timestamp":1760621908000},"score":1,"resource":{"primary":{"URL":"https:\/\/isevjournals.onlinelibrary.wiley.com\/doi\/10.1002\/jev2.12276"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,11]]},"references-count":101,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2022,11]]}},"alternative-id":["10.1002\/jev2.12276"],"URL":"https:\/\/doi.org\/10.1002\/jev2.12276","archive":["Portico"],"relation":{},"ISSN":["2001-3078","2001-3078"],"issn-type":[{"value":"2001-3078","type":"print"},{"value":"2001-3078","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,11]]},"article-number":"12276"}}