{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T08:32:56Z","timestamp":1790670776029,"version":"4.1.0"},"reference-count":36,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2022,12,5]],"date-time":"2022-12-05T00:00:00Z","timestamp":1670198400000},"content-version":"vor","delay-in-days":4,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["U01AI144673"],"award-info":[{"award-number":["U01AI144673"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004704","name":"National Research Council of Thailand","doi-asserted-by":"publisher","award":["N42A650870"],"award-info":[{"award-number":["N42A650870"]}],"id":[{"id":"10.13039\/501100004704","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Extracellular Vesicle"],"published-print":{"date-parts":[[2022,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The interaction of SARS\u2010CoV\u20102 infection with extracellular vesicles (EVs) is of particular interest at the moment. Studying SARS\u2010CoV\u20102 contaminated\u2010EV isolates in instruments located outside of the biosafety level\u20103 (BSL\u20103) environment requires knowing how viral inactivation methods affect the structure and function of extracellular vesicles (EVs). Therefore, three common viral inactivation methods, ultraviolet\u2010C (UVC; 1350 mJ\/cm<jats:sup>2<\/jats:sup>), \u03b2\u2010propiolactone (BPL; 0.005%), heat (56\u00b0C, 45\u00a0min) were performed on defined EV particles and their proteins, RNAs, and function. Small EVs were isolated from the supernatant of SARS\u2010CoV\u20102\u2010infected human lung epithelial Calu\u20103 cells by stepwise centrifugation, ultrafiltration and qEV size\u2010exclusion chromatography. The EV isolates contained SARS\u2010CoV\u20102. UVC, BPL and heat completely abolished SARS\u2010CoV\u20102 infectivity of the contaminated EVs. Particle detection by electron microscopy and nanoparticle tracking was less affected by UVC and BPL than heat treatment. Western blot analysis of EV markers was not affected by any of these three methods. UVC reduced SARS\u2010CoV\u20102 spike detectability by quantitative RT\u2010PCR and slightly altered EV\u2010derived \u03b2\u2010actin detection. Fibroblast migration\u2010wound healing activity of the SARS\u2010CoV\u20102 contaminated\u2010EV isolate was only retained after UVC treatment. In conclusion, specific viral inactivation methods are compatible with specific measures in SARS\u2010CoV\u20102 contaminated\u2010EV isolates. UVC treatment seems preferable for studying functions of EVs released from SARS\u2010CoV\u20102 infected cells.<\/jats:p>","DOI":"10.1002\/jev2.12291","type":"journal-article","created":{"date-parts":[[2022,12,5]],"date-time":"2022-12-05T09:40:42Z","timestamp":1670233242000},"source":"Crossref","is-referenced-by-count":17,"title":["Comparison of viral inactivation methods on the characteristics of extracellular vesicles from SARS\u2010CoV\u20102 infected human lung epithelial cells"],"prefix":"10.1002","volume":"11","author":[{"given":"Supasek","family":"Kongsomros","sequence":"first","affiliation":[{"name":"Chakri Naruebodindra Medical Institute, Faculty of Medicine Ramathibodi Hospital Mahidol University  Samut Prakan Thailand"},{"name":"Pediatric Translational Research Unit, Department of Pediatrics Faculty of Medicine Ramathibodi Hospital, Mahidol University  Bangkok Thailand"},{"name":"Department of Microbiology, Faculty of Science Mahidol University  Bangkok 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Epidemiology, Department of Environmental and Public Health Sciences University of Cincinnati College of Medicine  Cincinnati Ohio USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,12,5]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jobb.2020.12.001"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamem.2013.09.021"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.3390\/ma14051075"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.3390\/v12101200"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.3389\/fbioe.2020.00146"},{"key":"e_1_2_8_7_1","unstructured":"Centers for Disease Control and Prevention. 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