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Here, we identified that the glycoproteins CD147 and CD98 define subpopulations of EVs that are distinct from classical tetraspanin<jats:sup>+<\/jats:sup> EVs in their biogenesis. Notably, we identified that CD147<jats:sup>+<\/jats:sup> EVs have substantially higher microRNA (miRNA) content than tetraspanin<jats:sup>+<\/jats:sup> EVs and are selectively enriched in miRNA through the interaction of CD147 with heterogeneous nuclear ribonucleoprotein A2\/B1. Studies using mouse xenograft models showed that CD147<jats:sup>+<\/jats:sup> EVs predominantly derive from cancer cells, whereas the majority of tetraspanin<jats:sup>+<\/jats:sup> EVs are not of cancer cell origin. Circulating CD147<jats:sup>+<\/jats:sup> EVs, but not tetraspanin<jats:sup>+<\/jats:sup> EVs, were significantly increased in prevalence in patients with ovarian and renal cancers as compared to healthy individuals and patients with benign conditions. Furthermore, we found that isolating miRNAs from body fluids by CD147 immunocapture increases the sensitivity of detecting cancer cell\u2010specific miRNAs, and that circulating miRNAs isolated by CD147 immunocapture more closely reflect the tumor miRNA signature than circulating miRNAs isolated by conventional methods. Collectively, our findings reveal that CD147 defines miRNA\u2010enriched, cancer cell\u2010derived EVs, and that CD147 immunocapture could be an effective approach to isolate cancer\u2010derived miRNAs for liquid biopsy.<\/jats:p>","DOI":"10.1002\/jev2.12318","type":"journal-article","created":{"date-parts":[[2023,3,27]],"date-time":"2023-03-27T23:29:15Z","timestamp":1679959755000},"source":"Crossref","is-referenced-by-count":35,"title":["The glycoprotein CD147 defines miRNA\u2010enriched extracellular vesicles that derive from cancer cells"],"prefix":"10.1002","volume":"12","author":[{"given":"Song Yi","family":"Ko","sequence":"first","affiliation":[{"name":"Department of Molecular and Cellular Oncology University of Texas MD Anderson Cancer Center  Houston Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"WonJae","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Molecular and Cellular Oncology University of Texas MD Anderson Cancer 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