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Many HGSC patients have excess fluid in the peritoneum called ascites. Ascites is a tumour microenvironment (TME) containing various cells, proteins and extracellular vesicles (EVs). We isolated EVs from patients\u2019 ascites by orthogonal methods and analyzed them by mass spectrometry. We identified not only a set of \u2018core ascitic EV\u2010associated proteins\u2019 but also defined their subset unique to HGSC ascites. Using single\u2010cell RNA sequencing data, we mapped the origin of HGSC\u2010specific EVs to different types of cells present in ascites. Surprisingly, EVs did not come predominantly from tumour cells but from non\u2010malignant cell types such as macrophages and fibroblasts. Flow cytometry of ascitic cells in combination with analysis of EV protein composition in matched samples showed that analysis of cell type\u2010specific EV markers in HGSC has more substantial prognostic potential than analysis of ascitic cells. To conclude, we provide evidence that proteomic analysis of EVs can define the cellular composition of HGSC TME. This finding opens numerous avenues both for a better understanding of EV's role in tumour promotion\/prevention and for improved HGSC diagnostics.<\/jats:p>","DOI":"10.1002\/jev2.12420","type":"journal-article","created":{"date-parts":[[2024,3,16]],"date-time":"2024-03-16T02:36:32Z","timestamp":1710556592000},"source":"Crossref","is-referenced-by-count":32,"title":["Proteomic analysis of ascitic extracellular vesicles describes tumour microenvironment and predicts patient survival in ovarian cancer"],"prefix":"10.1002","volume":"13","author":[{"given":"Anna","family":"Vyhl\u00eddalov\u00e1 Kotrbov\u00e1","sequence":"first","affiliation":[{"name":"Department of Experimental Biology, Faculty of Science Masaryk University  Brno Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Krist\u00edna","family":"G\u00f6m\u00f6ryov\u00e1","sequence":"additional","affiliation":[{"name":"Department of Experimental Biology, Faculty of Science Masaryk University  Brno Czech 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