{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T15:26:03Z","timestamp":1791473163026,"version":"4.3.4"},"reference-count":36,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2024,6,29]],"date-time":"2024-06-29T00:00:00Z","timestamp":1719619200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Extracellular Vesicle"],"published-print":{"date-parts":[[2024,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Haematopoiesis dysregulation with the presence of immature myeloid and erythroid immunosuppressive cells are key characteristics of the immune escape phase of tumour development. Here, the role of in vitro generated B16F10 tumour cell\u2010derived extracellular vesicles (tEVs) as indirect cellular communicators, participating in tumour\u2010induced dysregulation of haematopoiesis, was explored. The isolated tEVs displayed features of small EVs with a size range of 100\u2013200\u00a0nm, expressed the common EV markers CD63, CD9, and Alix, and had a spherical shape with a lipid bilayer membrane. Proteomic profiling revealed significant levels of angiogenic factors, particularly vascular endothelial growth factor (VEGF), osteopontin, and tissue factor, associated with the tEVs. Systemic administration of these tEVs in syngeneic mice induced splenomegaly and disrupted haematopoiesis, leading to extramedullary haematopoiesis, expansion of splenic immature erythroid progenitors, reduced bone marrow cellularity, medullary expansion of granulocytic myeloid suppressor cells, and the development of anaemia. These effects closely mirrored those observed in tumour\u2010bearing mice and were not seen after heat inactivating the tEVs. In vitro studies demonstrated that tEVs independently induced the expansion of bone marrow granulocytic myeloid suppressor cells and B cells while reducing the frequency of cells in the erythropoietic lineage. These effects of tEVs were significantly abrogated by the blockade of VEGF or heat inactivation. Our findings underscore the important role of tEVs in dysregulating haematopoiesis during the immune escape phase of cancer immunoediting, suggesting their potential as targets for addressing immune evasion and reinstating normal hematopoietic processes.<\/jats:p>","DOI":"10.1002\/jev2.12471","type":"journal-article","created":{"date-parts":[[2024,6,30]],"date-time":"2024-06-30T00:19:52Z","timestamp":1719706792000},"source":"Crossref","is-referenced-by-count":18,"title":["Extracellular vesicles originating from melanoma cells promote dysregulation in haematopoiesis as a component of cancer immunoediting"],"prefix":"10.1002","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4785-0796","authenticated-orcid":false,"given":"Doste R.","family":"Mamand","sequence":"first","affiliation":[{"name":"Biomolecular and Cellular Medicine (BCM), Department of Laboratory Medicine Karolinska Institutet  Huddinge Sweden"},{"name":"Breast Center, Karolinska Comprehensive Cancer Center, Karolinska University Hospital, Stockholm, Sweden Karolinska Institute  Stockholm Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Safa","family":"Bazaz","sequence":"additional","affiliation":[{"name":"Biomolecular and Cellular Medicine (BCM), Department of Laboratory Medicine Karolinska Institutet  Huddinge Sweden"},{"name":"Department of Cellular Therapy and Allogeneic Stem Cell Transplantation (CAST) Karolinska University Hospital Huddinge and Karolinska Comprehensive Cancer Center  Stockholm Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dara K.","family":"Mohammad","sequence":"additional","affiliation":[{"name":"Center for Hematology and Regenerative Medicine (HERM), Department of Medicine Huddinge Karolinska Institutet  Stockholm Sweden"},{"name":"College of Agricultural Engineering Sciences Salahaddin University\u2010Erbil  Erbil Iraq"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0202-1211","authenticated-orcid":false,"given":"Xiuming","family":"Liang","sequence":"additional","affiliation":[{"name":"Biomolecular and Cellular Medicine (BCM), Department of Laboratory Medicine Karolinska Institutet  Huddinge Sweden"},{"name":"Department of Cellular Therapy and Allogeneic Stem Cell Transplantation (CAST) Karolinska University Hospital Huddinge and Karolinska Comprehensive Cancer Center  Stockholm Sweden"},{"name":"Karolinska ATMP Center, ANA Futura, Huddinge, Sweden Karolinska Institute  Stockholm Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Svetlana","family":"Pavlova","sequence":"additional","affiliation":[{"name":"Biomolecular and Cellular Medicine (BCM), Department of Laboratory Medicine Karolinska Institutet  Huddinge Sweden"},{"name":"Department of Cellular Therapy and Allogeneic Stem Cell Transplantation (CAST) Karolinska University Hospital Huddinge and Karolinska Comprehensive Cancer Center  Stockholm Sweden"},{"name":"Karolinska ATMP Center, ANA Futura, Huddinge, Sweden Karolinska Institute  Stockholm Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carsten","family":"Mim","sequence":"additional","affiliation":[{"name":"Department of Protein Science KTH Royal Institute of Technology  Stockholm Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1771-1346","authenticated-orcid":false,"given":"Susanne","family":"Gabrielsson","sequence":"additional","affiliation":[{"name":"Division of Immunology and Allergy, Department of Medicine Solna Karolinska Institutet  Solna Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3653-7710","authenticated-orcid":false,"given":"Joel Z.","family":"Nordin","sequence":"additional","affiliation":[{"name":"Biomolecular and Cellular Medicine (BCM), Department of Laboratory Medicine Karolinska Institutet  Huddinge Sweden"},{"name":"Department of Clinical Immunology and Transfusion Medicine (KITM) Karolinska University Hospital  Stockholm Sweden"},{"name":"Karolinska ATMP Center, ANA Futura, Huddinge, Sweden Karolinska Institute  Stockholm Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oscar P. 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