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Emerging as a promising avenue for biomarker development, proteomic technology leveraging liquid biopsy from small extracellular vesicles (sEV) offers new insights. Here, we evaluated various methods for sEV isolation and identified polysaccharide chitosan (CS) as an optimal approach. Subsequently, we employed optimized CS\u2010based magnetic beads (Mag\u2010CS) for sEV separation from serum samples of healthy controls, CHB, LC, and HBV\u2010HCC patients. Leveraging data\u2010independent acquisition mass spectrometry coupled with machine learning, we uncovered potential vesicular protein biomarker signatures (KNG1, F11, KLKB1, CAPNS1, CDH1, CPN2, NME2) capable of distinguishing HBV\u2010HCC from CHB, LC, and non\u2010HCC conditions. Collectively, our findings highlight the utility of Mag\u2010CS\u2010based sEV isolation for identifying early detection biomarkers in HBV\u2010HCC.<\/jats:p>","DOI":"10.1002\/jev2.12499","type":"journal-article","created":{"date-parts":[[2024,8,29]],"date-time":"2024-08-29T08:15:11Z","timestamp":1724919311000},"source":"Crossref","is-referenced-by-count":17,"title":["The trajectory of vesicular proteomic signatures from HBV\u2010HCC by chitosan\u2010magnetic bead\u2010based separation and DIA\u2010proteomic analysis"],"prefix":"10.1002","volume":"13","author":[{"given":"Lin","family":"Cao","sequence":"first","affiliation":[{"name":"Institute of Hematology Provincial Key Laboratory of Biotechnology, School of Medicine Northwest University  Xi'an Shaanxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yue","family":"Zhou","sequence":"additional","affiliation":[{"name":"Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, Provincial Key Laboratory of Biotechnology, College of Life Sciences Northwest University  Xi'an Shaanxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuai","family":"Lin","sequence":"additional","affiliation":[{"name":"Department of Oncology The Second Affiliated Hospital of Xi'an Jiaotong University  Xi'an Shaanxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunyan","family":"Yang","sequence":"additional","affiliation":[{"name":"Institute of Basic and Translational Medicine Xi'an Medical University  Xi'an Shaanxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zixuan","family":"Guan","sequence":"additional","affiliation":[{"name":"Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, Provincial Key Laboratory of Biotechnology, College of Life Sciences Northwest University  Xi'an Shaanxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaofan","family":"Li","sequence":"additional","affiliation":[{"name":"Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, Provincial Key Laboratory of Biotechnology, College of Life Sciences Northwest University  Xi'an Shaanxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shujie","family":"Yang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, Provincial Key Laboratory of Biotechnology, College of Life Sciences Northwest University  Xi'an Shaanxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tong","family":"Gao","sequence":"additional","affiliation":[{"name":"Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, Provincial Key Laboratory of Biotechnology, College of Life Sciences Northwest University  Xi'an Shaanxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiazhen","family":"Zhao","sequence":"additional","affiliation":[{"name":"Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, Provincial Key Laboratory of Biotechnology, College of Life Sciences Northwest University  Xi'an Shaanxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ning","family":"Fan","sequence":"additional","affiliation":[{"name":"Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, Provincial Key Laboratory of Biotechnology, College of Life Sciences Northwest University  Xi'an Shaanxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanan","family":"Song","sequence":"additional","affiliation":[{"name":"Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, Provincial Key Laboratory of Biotechnology, College of Life Sciences Northwest University  Xi'an Shaanxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dongmin","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences Xi'an Jiaotong University Health Science Center  Xi'an Shaanxi P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiang","family":"Li","sequence":"additional","affiliation":[{"name":"Institute of Hematology Provincial Key Laboratory of Biotechnology, School of Medicine Northwest University  Xi'an Shaanxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhuo","family":"Li","sequence":"additional","affiliation":[{"name":"Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, Provincial Key Laboratory of Biotechnology, College of Life Sciences Northwest University  Xi'an Shaanxi China"},{"name":"Department of Laboratory Medicine The First Affiliated Hospital of Xi'an Medical University  Xi'an Shaanxi P.R. 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