{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T20:25:25Z","timestamp":1792441525632,"version":"4.5.0"},"reference-count":74,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2024,10,8]],"date-time":"2024-10-08T00:00:00Z","timestamp":1728345600000},"content-version":"vor","delay-in-days":7,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Extracellular Vesicle"],"published-print":{"date-parts":[[2024,10]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Despite the advances in the understanding of Huntington's disease (HD), there is a need for molecular biomarkers to categorize mutation carriers during the preclinical stage of the disease preceding functional decline. Small RNAs (sRNAs) are a promising source of biomarkers since their expression levels are highly sensitive to pathobiological processes. Here, using an optimized method for plasma extracellular vesicles (EVs) purification and an exhaustive analysis pipeline of sRNA sequencing data, we show that EV\u2010sRNAs are downregulated early in mutation carriers and that this deregulation is associated with premanifest cognitive performance. Seven candidate sRNAs (tRF\u2010Glu\u2010CTC, tRF\u2010Gly\u2010GCC, miR\u2010451a, miR\u201021\u20105p, miR\u201026a\u20105p, miR\u201027a\u20103p and let7a\u20105p) were validated in additional subjects, showing a significant diagnostic accuracy at premanifest stages. Of these, miR\u201021\u20105p was significantly decreased over time in a longitudinal study; and miR\u201021\u20105p and miR\u201026a\u20105p levels correlated with cognitive changes in the premanifest cohort. In summary, the present results suggest that deregulated plasma EV\u2010sRNAs define an early biosignature in mutation carriers with specific species highlighting the progression and cognitive changes occurring at the premanifest stage.<\/jats:p>","DOI":"10.1002\/jev2.12522","type":"journal-article","created":{"date-parts":[[2024,10,8]],"date-time":"2024-10-08T12:39:43Z","timestamp":1728391183000},"source":"Crossref","is-referenced-by-count":18,"title":["Small RNAs in plasma extracellular vesicles define biomarkers of premanifest changes in Huntington's disease"],"prefix":"10.1002","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2693-0700","authenticated-orcid":false,"given":"Marina","family":"Herrero\u2010Lorenzo","sequence":"first","affiliation":[{"name":"Department of Biomedicine Faculty of Medicine, Institute of Neurosciences University of Barcelona  Barcelona Catalunya Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9835-0484","authenticated-orcid":false,"given":"Jes\u00fas","family":"P\u00e9rez\u2010P\u00e9rez","sequence":"additional","affiliation":[{"name":"Movement Disorders Unit Neurology Department Sant Pau Hospital  Barcelona Catalunya Spain"},{"name":"Biomedical Research Institute (IIB\u2010Sant Pau)  Barcelona Catalunya Spain"},{"name":"Center\u00a0for Networked Biomedical Research in\u00a0Neurodegenerative Diseases (CIBERNED)  Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5416-3177","authenticated-orcid":false,"given":"Georgia","family":"Escaram\u00eds","sequence":"additional","affiliation":[{"name":"Department of Biomedicine Faculty of Medicine, Institute of Neurosciences University of Barcelona  Barcelona Catalunya Spain"},{"name":"Biomedical Research Networking\u00a0Center\u00a0for\u00a0Epidemiology and Public Health (CIBERESP) Spanish Ministry of Science and Innovation  Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0125-7249","authenticated-orcid":false,"given":"Sa\u00fcl","family":"Mart\u00ednez\u2010Horta","sequence":"additional","affiliation":[{"name":"Movement Disorders Unit Neurology Department Sant Pau Hospital  Barcelona Catalunya Spain"},{"name":"Biomedical Research Institute (IIB\u2010Sant Pau)  Barcelona Catalunya Spain"},{"name":"Center\u00a0for Networked Biomedical Research in\u00a0Neurodegenerative Diseases (CIBERNED)  Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0641-2001","authenticated-orcid":false,"given":"Roc\u00edo","family":"P\u00e9rez\u2010Gonz\u00e1lez","sequence":"additional","affiliation":[{"name":"Movement Disorders Unit Neurology Department Sant Pau Hospital  Barcelona Catalunya Spain"},{"name":"Biomedical Research Institute (IIB\u2010Sant Pau)  Barcelona Catalunya Spain"},{"name":"Center\u00a0for Networked Biomedical Research in\u00a0Neurodegenerative Diseases (CIBERNED)  Madrid Spain"},{"name":"Alicante Institute for Health and Biomedical Research (ISABIAL) and Neuroscience Institute  Alicante Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elisa","family":"Rivas\u2010Asensio","sequence":"additional","affiliation":[{"name":"Movement Disorders Unit Neurology Department Sant Pau Hospital  Barcelona Catalunya Spain"},{"name":"Biomedical Research Institute (IIB\u2010Sant Pau)  Barcelona Catalunya Spain"},{"name":"Center\u00a0for Networked Biomedical Research in\u00a0Neurodegenerative Diseases (CIBERNED)  Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4870-1431","authenticated-orcid":false,"given":"Jaime","family":"Kulisevsky","sequence":"additional","affiliation":[{"name":"Movement Disorders Unit Neurology Department Sant Pau Hospital  Barcelona Catalunya Spain"},{"name":"Biomedical Research Institute (IIB\u2010Sant Pau)  Barcelona Catalunya Spain"},{"name":"Center\u00a0for Networked Biomedical Research in\u00a0Neurodegenerative Diseases (CIBERNED)  Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9530-2751","authenticated-orcid":false,"given":"Ana","family":"G\u00e1mez\u2010Valero","sequence":"additional","affiliation":[{"name":"Department of Biomedicine Faculty of Medicine, Institute of Neurosciences University of Barcelona  Barcelona Catalunya Spain"},{"name":"Biomedical Research Networking\u00a0Center\u00a0for\u00a0Epidemiology and Public Health (CIBERESP) Spanish Ministry of Science and Innovation  Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1030-3158","authenticated-orcid":false,"given":"Eul\u00e0lia","family":"Mart\u00ed","sequence":"additional","affiliation":[{"name":"Department of Biomedicine Faculty of Medicine, Institute of Neurosciences University of Barcelona  Barcelona Catalunya Spain"},{"name":"Biomedical Research Networking\u00a0Center\u00a0for\u00a0Epidemiology and Public Health (CIBERESP) Spanish Ministry of Science and Innovation  Madrid Spain"},{"name":"August Pi i Sunyer Biomedical research Institute (IDIBAPS), Barcelona  Catalunya Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,10,8]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1009951"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1019055108"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrdp.2015.5"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/ajmg.b.32719"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1474-4422(17)30124-2"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.3233\/JHD-140115"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells10123355"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2216330120"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00401-021-02272-9"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phrs.2016.05.005"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/bts635"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.3389\/fncel.2021.705348"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12031-022-02012-y"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/jev2.12043"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a007476"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-nutr-071715-050711"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.wneu.2019.05.194"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40035-019-0169-5"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep33641"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2008323117"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnagi.2022.987174"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41576-018-0071-5"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12920-015-0083-3"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/mds.26457"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2019.02.029"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/nn.4256"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0190550"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.201904113"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1080\/15476286.2016.1249092"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1186\/gb-2013-14-7-r78"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13059-014-0550-8"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12964-021-00757-4"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(93)90585-E"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.3000363"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkq575"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkq575"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.14806\/ej.17.1.200"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1002\/cpsc.82"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-022-04410-z"},{"key":"e_1_2_8_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2019.02.018"},{"key":"e_1_2_8_42_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btr527"},{"key":"e_1_2_8_43_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btv632"},{"key":"e_1_2_8_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.parkreldis.2021.04.017"},{"key":"e_1_2_8_45_1","doi-asserted-by":"publisher","DOI":"10.1136\/jnnp.2007.128728"},{"key":"e_1_2_8_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-801893-4.00006-7"},{"key":"e_1_2_8_47_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40478-022-01407-7"},{"key":"e_1_2_8_48_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms22041561"},{"key":"e_1_2_8_49_1","doi-asserted-by":"publisher","DOI":"10.1212\/WNL.0000000000004844"},{"key":"e_1_2_8_50_1","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.abc2888"},{"key":"e_1_2_8_51_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cels.2019.03.004"},{"key":"e_1_2_8_52_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00062-022-01150-5"},{"key":"e_1_2_8_53_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1474-4422(20)30143-5"},{"key":"e_1_2_8_54_1","doi-asserted-by":"publisher","DOI":"10.1155\/2013\/253957"},{"key":"e_1_2_8_55_1","doi-asserted-by":"publisher","DOI":"10.1212\/WNL.0000000000004743"},{"key":"e_1_2_8_56_1","doi-asserted-by":"publisher","DOI":"10.18632\/aging.100486"},{"key":"e_1_2_8_57_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41556-021-00652-7"},{"key":"e_1_2_8_58_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep12166"},{"key":"e_1_2_8_59_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2019.03.024"},{"key":"e_1_2_8_60_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41582-020-0389-4"},{"key":"e_1_2_8_61_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2019.01.039"},{"key":"e_1_2_8_62_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1474-4422(22)00120-X"},{"key":"e_1_2_8_63_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jns.2013.10.002"},{"key":"e_1_2_8_64_1","unstructured":"Th\u00e9ry C. Witwer K. W. Aikawa E. Alcaraz M. J. Anderson J. D. Andriantsitohaina R. Antoniou A. Arab T. Archer F. Atkin\u2010Smith G. K. Ayre D. C. Bach J. M. Bachurski D. Baharvand H. Balaj L. Baldacchino S. Bauer N. N. Baxter A. A. Bebawy M. \u2026Zuba\u2010Surma E. K.(2018).Minimal information for studies of extracellular vesicles 2018 (MISEV2018): A position statement of the International Society for Extracellular Vesicles and update of the MISEV2014 guidelines.119."},{"key":"e_1_2_8_65_1","doi-asserted-by":"publisher","DOI":"10.1261\/rna.1232808"},{"key":"e_1_2_8_66_1","doi-asserted-by":"publisher","DOI":"10.1080\/15476286.2020.1729584"},{"key":"e_1_2_8_67_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkv432"},{"key":"e_1_2_8_68_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkaa674"},{"key":"e_1_2_8_69_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.4185"},{"key":"e_1_2_8_70_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncb2210"},{"key":"e_1_2_8_71_1","doi-asserted-by":"publisher","DOI":"10.1017\/erm.2023.19"},{"key":"e_1_2_8_72_1","doi-asserted-by":"publisher","DOI":"10.3389\/fneur.2020.551411"},{"key":"e_1_2_8_73_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1474-4422(11)70070-9"},{"key":"e_1_2_8_74_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI80743"},{"key":"e_1_2_8_75_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep44061"}],"container-title":["Journal of Extracellular Vesicles"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/isevjournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jev2.12522","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,16]],"date-time":"2025-10-16T17:31:34Z","timestamp":1760635894000},"score":1,"resource":{"primary":{"URL":"https:\/\/isevjournals.onlinelibrary.wiley.com\/doi\/10.1002\/jev2.12522"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,10]]},"references-count":74,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2024,10]]}},"alternative-id":["10.1002\/jev2.12522"],"URL":"https:\/\/doi.org\/10.1002\/jev2.12522","archive":["Portico"],"relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/2023.12.01.568823","asserted-by":"object"}]},"ISSN":["2001-3078","2001-3078"],"issn-type":[{"value":"2001-3078","type":"print"},{"value":"2001-3078","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,10]]},"article-number":"e12522"}}