{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T17:58:49Z","timestamp":1790099929909,"version":"4.0.1"},"reference-count":26,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2024,10,14]],"date-time":"2024-10-14T00:00:00Z","timestamp":1728864000000},"content-version":"vor","delay-in-days":13,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["52072418"],"award-info":[{"award-number":["52072418"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["52373165"],"award-info":[{"award-number":["52373165"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003453","name":"Natural Science Foundation of Guangdong Province","doi-asserted-by":"publisher","award":["2022A1515010977"],"award-info":[{"award-number":["2022A1515010977"]}],"id":[{"id":"10.13039\/501100003453","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Extracellular Vesicle"],"published-print":{"date-parts":[[2024,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Bacterial infections, especially those caused by multidrug\u2010resistant pathogens, pose a significant threat to public health. Vaccines are a crucial tool in fighting these infections; however, no clinically available vaccine exists for the most common bacterial infections, such as those caused by <jats:italic>Pseudomonas aeruginosa<\/jats:italic>. Herein, a multiantigenic antibacterial nanovaccine (AuNP@HMV@SPs) is reported to combat <jats:italic>P. aeruginosa<\/jats:italic> infections. This nanovaccine utilizes the hybrid membrane vesicles (HMVs) created by fusing macrophage membrane vesicles (MMVs) with bacterial outer membrane vesicles (OMVs). The HMVs mitigate the toxic effects of both OMVs and bacterial secreted toxins (SP) adsorbed on the surface of MMVs, while preserving their stimulating properties. Gold nanoparticles (AuNPs) are utilized as adjuvant to enhance immune response without comprising safety. The nanovaccine AuNP@HMV@SPs induces robust humoral and cellular immune responses, leading to destruction of bacterial cells and neutralization of their secreted toxins. In murine models of septicemia and pneumonia caused by <jats:italic>P. aeruginosa<\/jats:italic>, AuNP@HMV@SPs exhibits superior prophylactic efficacy compared to control groups including OMVs, or MMVs@SPs and HMV@SPs, achieving 100% survival in septicemia and\u00a0&gt;\u00a099.9% reduction in lung bacterial load in pneumonia. This study highlights AuNP@HMV@SPs as a safe and effective antibacterial nanovaccine, targeting both bacteria and their secreted toxins, and offers a promising platform for developing multiantigenic antibacterial vaccines against multidrug\u2010resistant pathogens.<\/jats:p>","DOI":"10.1002\/jev2.12524","type":"journal-article","created":{"date-parts":[[2024,10,14]],"date-time":"2024-10-14T06:46:18Z","timestamp":1728888378000},"source":"Crossref","is-referenced-by-count":28,"title":["A multiantigenic antibacterial nanovaccine utilizing hybrid membrane vesicles for combating <i>Pseudomonas aeruginosa<\/i> infections"],"prefix":"10.1002","volume":"13","author":[{"given":"Xinran","family":"Peng","sequence":"first","affiliation":[{"name":"School of Pharmaceutical Sciences (Shenzhen) Shenzhen Campus of Sun Yat\u2010sen University  Shenzhen PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuanjing","family":"Luo","sequence":"additional","affiliation":[{"name":"School of Pharmaceutical Sciences (Shenzhen) Shenzhen Campus of Sun Yat\u2010sen University  Shenzhen PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Yang","sequence":"additional","affiliation":[{"name":"School of Pharmaceutical Sciences (Shenzhen) Shenzhen Campus of Sun Yat\u2010sen University  Shenzhen PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi Yan","family":"Yang","sequence":"additional","affiliation":[{"name":"Bioprocessing Technology Institute (BTI), Agency for Science, Technology and Research (A*STAR)  Singapore Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peiyan","family":"Yuan","sequence":"additional","affiliation":[{"name":"School of Pharmaceutical Sciences (Shenzhen) Shenzhen Campus of Sun Yat\u2010sen University  Shenzhen PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinhai","family":"Chen","sequence":"additional","affiliation":[{"name":"Institute of Infectious Diseases, Shenzhen Bay Laboratory  Shenzhen China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guo\u2010Bao","family":"Tian","sequence":"additional","affiliation":[{"name":"Department of Immunology and Microbiology, Advanced Medical Technology Center, The First Affiliated Hospital, Zhongshan School of Medicine Sun Yat\u2010sen University  Guangzhou China"},{"name":"Key Laboratory of Tropical Diseases Control (Sun Yat\u2010sen University), Ministry of Education  Guangzhou China"},{"name":"School of Medicine Sun Yat\u2010Sen University  Shenzhen China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6993-3034","authenticated-orcid":false,"given":"Xin","family":"Ding","sequence":"additional","affiliation":[{"name":"School of Medicine Sun Yat\u2010Sen University  Shenzhen China"},{"name":"State Key Laboratory of Anti\u2010Infective Drug Discovery and Development; 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