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We demonstrate that <jats:italic>Ascaris<\/jats:italic> EVs are efficiently internalised by monocytes in human peripheral blood mononuclear cells and increase the percentage of classical monocytes. Furthermore, EV treatment of monocytes induced a novel anti\u2010inflammatory phenotype characterised by CD14<jats:sup>+<\/jats:sup>, CD16<jats:sup>\u2212<\/jats:sup>, CC chemokine receptor 2 (CCR2<jats:sup>\u2212<\/jats:sup>) and programmed death\u2010ligand 1 (PD\u2010L1)<jats:sup>+<\/jats:sup> cells. In addition, <jats:italic>Ascaris<\/jats:italic> EVs induced T cell anergy in a monocyte\u2010dependent mechanism. Targeting professional phagocytes to induce both direct and indirect pathways of immune modulation presents a highly novel and efficient mechanism of EV\u2010mediated host\u2010parasite communication. Intra\u2010peritoneal administration of EVs induced protection against gut inflammation in the dextran sodium sulphate model of colitis in mice. <jats:italic>Ascaris<\/jats:italic> EVs were shown to affect circulating immune cells and protect against gut inflammation; this highlights their potential as a subject for further investigation in inflammatory conditions driven by dysregulated immune responses. However, their clinical translation would require further studies and careful consideration of ethical implications.<\/jats:p>","DOI":"10.1002\/jev2.70027","type":"journal-article","created":{"date-parts":[[2025,1,16]],"date-time":"2025-01-16T03:05:07Z","timestamp":1736996707000},"source":"Crossref","is-referenced-by-count":8,"title":["Helminth extracellular vesicles co\u2010opt host monocytes to drive T cell anergy"],"prefix":"10.1002","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7710-4391","authenticated-orcid":false,"given":"Anne","family":"Borup","sequence":"first","affiliation":[{"name":"Department of Clinical Medicine Aarhus University  Aarhus Denmark"},{"name":"Department of Infectious Diseases Aarhus University Hospital  Aarhus Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7950-955X","authenticated-orcid":false,"given":"Mohammad 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