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Extracellular vesicles (EVs) are key mediators involved in bacteria\u2010to\u2010host communication. However, their potential role in mediating addictive behaviour remains unexplored. This study investigates the role of gut microbiota\u2010derived bacterial extracellular vesicles (bEVs) in driving high alcohol consumption. bEVs were isolated from the gut microbiota of a high alcohol\u2010drinking rat strain (UChB rats), either ethanol\u2010na\u00efve or following chronic alcohol consumption and administered intraperitoneally or orally to alcohol\u2010rejecting male and female Wistar rats. Both types of UChB\u2010derived bEVs increased Wistar's voluntary alcohol consumption (three bottle choice test) up to 10\u2010fold (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.0001), indicating that bEVs are able and sufficient to transmit drinking behaviour across different rat strains. Molecular analysis revealed that bEVs administration did not induce systemic or brain inflammation in the recipient animals, suggesting that the increased alcohol intake triggered by UChB\u2010derived bEVs operates through an inflammation\u2010independent mechanism. Furthermore, we demonstrate that the vagus nerve mediates the bEV\u2010induced increase in alcohol consumption, as bilateral vagotomy completely abolished the high drinking behaviour induced by both intraperitoneally injected and orally administered bEVs. Thus, this study identifies bEVs as a novel mechanism underlying gut microbiota\u2010induced high alcohol intake in a vagus nerve\u2010dependent manner.<\/jats:p>","DOI":"10.1002\/jev2.70059","type":"journal-article","created":{"date-parts":[[2025,3,18]],"date-time":"2025-03-18T02:26:16Z","timestamp":1742264776000},"source":"Crossref","is-referenced-by-count":18,"title":["Gut Microbiota\u2010Derived Extracellular Vesicles Influence Alcohol Intake Preferences in Rats"],"prefix":"10.1002","volume":"14","author":[{"given":"Macarena","family":"D\u00edaz\u2010Ubilla","sequence":"first","affiliation":[{"name":"Center for Regenerative Medicine, Faculty of Medicine Cl\u00ednica Alemana\u2010Universidad del Desarrollo  Santiago Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7431-5046","authenticated-orcid":false,"given":"Aliosha I.","family":"Figueroa\u2010Vald\u00e9s","sequence":"additional","affiliation":[{"name":"Center of Interventional 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