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We engineered OMVs with cadherin 17 (CDH17) tumour\u2010targeting nanobodies, enhancing tumour selectivity and efficacy while reducing adverse effects. These engineered OMVs function as natural stimulator of interferon genes (STING) agonists, activating the cyclic GMP\u2010AMP synthase (cGAS)\u2010STING pathway in cancer cells and tumour\u2010associated macrophages (TAMs). Loading engineered OMVs with photoimmunotherapy photosensitisers further enhanced tumour inhibition and STING activation in TAMs. Combining nanobody\u2010engineered OMV\u2010mediated photoimmunotherapy with CD47 blockade effectively suppressed primary and metastatic tumours, establishing sustained antitumour immune memory. This study demonstrates the potential of nanobody\u2010engineered OMVs as STING agonists and provides insights into novel OMV\u2010based immunotherapeutic strategies harnessing the innate immune system against cancer. Our findings open new avenues for OMV applications in tumour immunotherapy, offering a promising approach to overcome current limitations in cancer treatment.<\/jats:p>","DOI":"10.1002\/jev2.70069","type":"journal-article","created":{"date-parts":[[2025,4,26]],"date-time":"2025-04-26T09:51:53Z","timestamp":1745661113000},"source":"Crossref","is-referenced-by-count":21,"title":["Nanobody Engineered and Photosensitiser Loaded Bacterial Outer Membrane Vesicles Potentiate Antitumour Immunity and Immunotherapy"],"prefix":"10.1002","volume":"14","author":[{"given":"Peng","family":"Xia","sequence":"first","affiliation":[{"name":"Zhongnan Hospital of Wuhan University, TaiKang Center for Life and Medical Sciences, Clinical Medicine Research Center for Minimally Invasive Procedure of Hepatobiliary &amp; Pancreatic Diseases of Hubei Province Wuhan University  Wuhan Hubei P. R. 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