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These studies are hampered by the lack of specific markers to identify the tdEVs. Here, we show that oncofoetal chondroitin sulphate, a malignancy\u2010associated glycosaminoglycan modification, is present on tdEVs and can be targeted by the malaria VAR2CSA protein or C9 antibody. Using a fluorescently labelled recombinant VAR2CSA protein, we identified EVs from cancer cells in vitro by super\u2010resolution fluorescence microscopy and flow cytometry, as well as in a proof\u2010of\u2010concept study using plasma samples from pancreatic adenocarcinoma patients. 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