{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T14:24:34Z","timestamp":1791815074092,"version":"4.3.4"},"reference-count":44,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2025,7,7]],"date-time":"2025-07-07T00:00:00Z","timestamp":1751846400000},"content-version":"vor","delay-in-days":6,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2025,7,1]],"date-time":"2025-07-01T00:00:00Z","timestamp":1751328000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100002794","name":"Cancerfonden","doi-asserted-by":"publisher","award":["243860Pj01H"],"award-info":[{"award-number":["243860Pj01H"]}],"id":[{"id":"10.13039\/501100002794","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004359","name":"Vetenskapsr\u00e5det","doi-asserted-by":"publisher","award":["2021\u201003538"],"award-info":[{"award-number":["2021\u201003538"]}],"id":[{"id":"10.13039\/501100004359","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Extracellular Vesicle"],"published-print":{"date-parts":[[2025,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Bacterial extracellular vesicles are spherical, nanosized structures with lipid bilayer membranes and can suppress tumour growth in cancer models. However, the efficacy of some of these models is limited. One potential way to enhance their effects is by loading the bacterial vesicles with immunostimulatory molecules. We have here utilised synthetic bacterial vesicles (SyBV), previously shown to have anti\u2010tumour effects but with reduced side effects. We hypothesized that loading SyBV with a STimulator of InterferoN Genes (STING) agonist can enhance anti\u2010tumour effects. SyBV were generated from\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    membranes through cell breakdown induced by lysozyme and ionic stress. The produced nanovesicles encapsulated the STING agonist (SyBV\n                    <jats:sup>STING<\/jats:sup>\n                    ). SyBV\n                    <jats:sup>STING<\/jats:sup>\n                    synergistically activated dendritic cells, leading to enhanced production of Interferon\u2010\u03b2. Furthermore, in vivo experiments showed that immunisation with SyBV\n                    <jats:sup>STING<\/jats:sup>\n                    synergistically suppresses melanoma and colon cancer growth by increasing the tumour infiltration of T cells. Intratumoural or subcutaneous injection of the SyBV resulted in retention in the tumour tissue over 24\u00a0h, but with some distribution to local lymph nodes. A toxicology experiment resulted in no histopathological concerns with SyBV\n                    <jats:sup>STING<\/jats:sup>\n                    . These findings show that SyBV loaded with a STING agonist synergistically enhance anti\u2010tumour immunity and may be a promising clinical immuno\u2010oncology tool.\n                  <\/jats:p>","DOI":"10.1002\/jev2.70117","type":"journal-article","created":{"date-parts":[[2025,7,7]],"date-time":"2025-07-07T03:25:44Z","timestamp":1751858744000},"source":"Crossref","is-referenced-by-count":9,"title":["STING Agonist Drug Delivery by Bacterial Extracellular Vesicles Induces Synergistic Immuno\u2010Oncology Responses and Efficient Inhibition of Tumour Growth"],"prefix":"10.1002","volume":"14","author":[{"given":"Jan","family":"L\u00f6tvall","sequence":"first","affiliation":[{"name":"Krefting Research Centre, Institute of Medicine, Sahlgrenska Academy University of Gothenburg  Gothenburg Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Negar","family":"Ordouzadeh","sequence":"additional","affiliation":[{"name":"Krefting Research Centre, Institute of Medicine, Sahlgrenska Academy University of Gothenburg  Gothenburg Sweden"},{"name":"Exocure Sweden AB  Gothenburg Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1714-3169","authenticated-orcid":false,"given":"Rossella","family":"Crescitelli","sequence":"additional","affiliation":[{"name":"Sahlgrenska Center for Cancer Research and Wallenberg Centre for Molecular and Translational Medicine, Department of Surgery, Institute of Clinical Sciences, Sahlgrenska Academy University of Gothenburg  Gothenburg Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meghshree","family":"Deshmukh","sequence":"additional","affiliation":[{"name":"Department of Rheumatology and Inflammation Research, Institute of Medicine, Sahlgrenska Academy University of Gothenburg  Gothenburg Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tao","family":"Jin","sequence":"additional","affiliation":[{"name":"Department of Rheumatology and Inflammation Research, Institute of Medicine, Sahlgrenska Academy University of Gothenburg  Gothenburg Sweden"},{"name":"Department of Rheumatology Sahlgrenska University Hospital  Gothenburg Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0902-7800","authenticated-orcid":false,"given":"Kyong\u2010Su","family":"Park","sequence":"additional","affiliation":[{"name":"Krefting Research Centre, Institute of Medicine, Sahlgrenska Academy University of Gothenburg  Gothenburg 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