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Here, we report a versatile delivery platform based on engineered ARRDC1\u2010mediated microvesicles (ARMMs) capable of delivering cargo to defined cell populations. By decorating ARMMs with engineered Nipah virus (NiV)\u2010derived fusion and attachment proteins conjugated to cell\u2010specific ligands, we enable selective binding and membrane fusion\u2010mediated cargo release. ARMMs functionalized with anti\u2010CD8 single\u2010chain variable fragment (scFv) delivered protein, mRNA, or CRISPR\u2010Cas9 base editor selectively to CD8\u207a T cells. Similarly, ARMMs displaying a designed ankyrin repeat protein (DARPin) targeting the GluA4 receptor enabled delivery to parvalbumin\u2010positive (PV\u207a) neurons. In vivo, administration of targeted ARMMs resulted in functional delivery to CD8\u207a splenocytes and PV\u207a cortical neurons in mice. These findings establish surface\u2010engineered ARMMs as a programmable and modular system for precision delivery of therapeutic macromolecules, with broad applicability in gene and RNA\u2010based medicine.<\/jats:p>","DOI":"10.1002\/jev2.70199","type":"journal-article","created":{"date-parts":[[2025,12,15]],"date-time":"2025-12-15T06:54:07Z","timestamp":1765781647000},"source":"Crossref","is-referenced-by-count":6,"title":["Targeted Intracellular Delivery via Precision Programming of ARRDC1\u2010Mediated Microvesicles"],"prefix":"10.1002","volume":"14","author":[{"given":"Zhi","family":"Qiao","sequence":"first","affiliation":[{"name":"Departments of Environmental Health &amp; Molecular Metabolism Harvard T.H. Chan School of Public Health  Boston Massachusetts USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sengjin","family":"Choi","sequence":"additional","affiliation":[{"name":"Departments of Environmental Health &amp; Molecular Metabolism Harvard T.H. 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