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Extracellular vesicles (EVs) in the pulmonary microenvironment significantly affect the immune system, but their underlying effects on Tregs are unclear. Here, we demonstrate increased endothelial cell\u2010derived EVs (CD31\n                    <jats:sup>+<\/jats:sup>\n                    EVs) in lipopolysaccharide (LPS)\u2010induced ALI models by single\u2010EV analysis. EVs from activated pulmonary endothelial cells (ECs) exhibit proinflammatory effects and suppress Treg induction. Exposure of Tregs to these EVs induces massive production of interleukin (IL)\u201021, which has been proven to reduce Foxp3 expression. Mechanistically, we find that Med1 enriched in these EVs can directly bind to the promoter region of the IL\u201021 gene thus activating IL\u201021 transcription in Tregs. Moreover, we confirm that suppressing Med1 accumulation in EVs from activated pulmonary ECs can reverse Treg differentiation, and alleviate lung inflammation. Finally, we observe a significant increase of EVs carrying Med1 in the BALF of patients with ARDS. Taken together, this study identifies that EV\u2010mediated pulmonary endothelium\u2010Treg communication is crucial for Treg suppression in ARDS and may provide potential therapeutic targets for the treatment of this fatal clinical syndrome.\n                  <\/jats:p>","DOI":"10.1002\/jev2.70235","type":"journal-article","created":{"date-parts":[[2026,2,9]],"date-time":"2026-02-09T07:35:25Z","timestamp":1770622525000},"source":"Crossref","is-referenced-by-count":2,"title":["Endothelium\u2010Treg Communication Through Extracellular Vesicle Transfer Exacerbates Acute Respiratory Distress Syndrome"],"prefix":"10.1002","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2264-5745","authenticated-orcid":false,"given":"Xu","family":"Liu","sequence":"first","affiliation":[{"name":"Jiangsu Provincial Key Laboratory of Critical Care Medicine, Department of Critical Care Medicine, Zhongda Hospital, School of Medicine Southeast University  Nanjing Jiangsu 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