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The fatty acid transporter, CD36 is central for ectopic liver fat accumulation in MASLD. Extracellular vesicle (EV) secretion is modulated by lipotoxicity and since circulating EVs expectedly represent phenotype of their cell of origin, EVs hold significant biomarker potential.<\/jats:p>\n                  <jats:p>\n                    Using high\u2010resolution flow cytometry, we compared levels of liver\u2010derived (ASGR1\n                    <jats:sup>+<\/jats:sup>\n                    \u2010EVs) and CD36\n                    <jats:sup>+<\/jats:sup>\n                    \u2010expressing EVs (CD36\n                    <jats:sup>+<\/jats:sup>\n                    \u2010EV) in individuals with obesity and MASLD (\n                    <jats:italic>n<\/jats:italic>\n                    = 36) with individuals with obesity (\n                    <jats:italic>n<\/jats:italic>\n                    = 25) or lean (\n                    <jats:italic>n<\/jats:italic>\n                    = 27) without MASLD. These EV\u2010populations were assessed for all groups at baseline and during weight loss intervention for the MASLD group.\n                  <\/jats:p>\n                  <jats:p>\n                    In the MASLD group at baseline, ASGR1\n                    <jats:sup>+<\/jats:sup>\n                    \u2010EVs were significantly increased compared with lean individuals, while CD36\n                    <jats:sup>+<\/jats:sup>\n                    \u2010EVs were significantly increased compared with either control group. During intervention, levels of both ASGR1\n                    <jats:sup>+<\/jats:sup>\n                    \u2010EVs, CD36\n                    <jats:sup>+<\/jats:sup>\n                    \u2010EVs and ASGR1\n                    <jats:sup>+<\/jats:sup>\n                    CD36\n                    <jats:sup>+<\/jats:sup>\n                    \u2010EVs were significantly decreased in the MASLD group already at 1 month and further reduced after 5 months. Finally, we found that CD36\n                    <jats:sup>+<\/jats:sup>\n                    \u2010EV levels correlated with liver fat content, total body fat, and markers of liver fibrosis.\n                  <\/jats:p>\n                  <jats:p>\n                    Our study is the first to report levels of circulating ASGR1\n                    <jats:sup>+<\/jats:sup>\n                    \u2010EVs and CD36\n                    <jats:sup>+<\/jats:sup>\n                    \u2010EV in MASLD. We hereby expand knowledge about EVs in developing MASLD, while advancing their biomarker potential.\n                  <\/jats:p>","DOI":"10.1002\/jev2.70257","type":"journal-article","created":{"date-parts":[[2026,3,17]],"date-time":"2026-03-17T14:35:40Z","timestamp":1773758140000},"source":"Crossref","is-referenced-by-count":6,"title":["Circulating Liver\u2010Derived and CD36\n                    <sup>+<\/sup>\n                    Extracellular Vesicles Correlate With Ectopic Liver Fat and Markers of Fibrosis in Metabolic Dysfunction\u2010Associated Steatotic Liver Disease and Decrease During Weight Loss Intervention"],"prefix":"10.1002","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5854-1086","authenticated-orcid":false,"given":"Maiken","family":"Mellergaard","sequence":"first","affiliation":[{"name":"Department of Clinical Biochemistry Aalborg University Hospital  Aalborg Denmark"},{"name":"Department of Clinical Medicine Aalborg University  Aalborg Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anders","family":"Askeland","sequence":"additional","affiliation":[{"name":"Department of Clinical Biochemistry Aalborg University Hospital  Aalborg Denmark"},{"name":"Department of Clinical Medicine Aalborg University  Aalborg Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rikke Wehner","family":"Rasmussen","sequence":"additional","affiliation":[{"name":"Department of Clinical Biochemistry Aalborg University Hospital  Aalborg Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-3250-8509","authenticated-orcid":false,"given":"Katja Lund","family":"Cliff","sequence":"additional","affiliation":[{"name":"Department of Clinical Biochemistry Aalborg University Hospital  Aalborg Denmark"},{"name":"Department of Clinical Medicine Aalborg University  Aalborg Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0748-6296","authenticated-orcid":false,"given":"Morten Hjuler","family":"Nielsen","sequence":"additional","affiliation":[{"name":"Department of Clinical Biochemistry Aalborg University Hospital  Aalborg Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rikke B\u00fclow","family":"Eschen","sequence":"additional","affiliation":[{"name":"Department of Clinical Biochemistry Aalborg University Hospital  Aalborg Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gunna","family":"Christiansen","sequence":"additional","affiliation":[{"name":"Department of Health Science and Technology Aalborg University  Aalborg Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Vestergaard","sequence":"additional","affiliation":[{"name":"Department of Clinical Medicine Aalborg University  Aalborg Denmark"},{"name":"Steno Diabetes Center North Denmark Aalborg University Hospital  Aalborg Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jens Br\u00f8ndum","family":"Fr\u00f8kj\u00e6r","sequence":"additional","affiliation":[{"name":"Department of Clinical Medicine Aalborg University  Aalborg Denmark"},{"name":"Department of Radiology Aalborg University Hospital  Aalborg Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aase","family":"Handberg","sequence":"additional","affiliation":[{"name":"Department of Clinical Biochemistry Aalborg University Hospital  Aalborg Denmark"},{"name":"Department of Clinical Medicine Aalborg University  Aalborg 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