{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T05:55:22Z","timestamp":1777874122578,"version":"3.51.4"},"reference-count":48,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2026,4,2]],"date-time":"2026-04-02T00:00:00Z","timestamp":1775088000000},"content-version":"vor","delay-in-days":1,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T00:00:00Z","timestamp":1775001600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100004359","name":"Vetenskapsr\u00e5det","doi-asserted-by":"publisher","award":["No.2021\u201303538"],"award-info":[{"award-number":["No.2021\u201303538"]}],"id":[{"id":"10.13039\/501100004359","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["LijianUifunding"],"award-info":[{"award-number":["LijianUifunding"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Extracellular Vesicle"],"published-print":{"date-parts":[[2026,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>The biological functions of extracellular vesicles (EVs) depend on their cellular source. Further, different subpopulations of EVs from the same cells carry different cargo, but differences in their biological functions are less understood. We here identify a very small EV subpopulation released by HEK293F cells (miniEVs). These EVs, in contrast to the larger EVs, were found to have anti\u2010inflammatory properties. Quantitative proteomics identified a potential anti\u2010inflammatory molecule, Syndecan\u20104 (SDC4), on the surface of the miniEVs, but not on larger EVs. We engineered HEK293F cells to overexpress SDC4, which results in the molecule being highly expressed in all EV subpopulations. Expression of SDC4, a proteoglycan, also increased the presence of heparan sulfate on the EV surface. Furthermore, these EVs were found to have potent anti\u2010inflammatory effects in vitro, which heparinase treatment could slightly reduce. Furthermore, the SDC4 EVs showed anti\u2010inflammatory effects in vivo in a model of peritonitis. We conclude that HEK293F miniEVs convey anti\u2010inflammatory properties, and SDC4\u2010expressing HEK293F\u2010EV potentially could become an anti\u2010inflammatory therapeutic.<\/jats:p>","DOI":"10.1002\/jev2.70266","type":"journal-article","created":{"date-parts":[[2026,4,2]],"date-time":"2026-04-02T17:06:01Z","timestamp":1775149561000},"source":"Crossref","is-referenced-by-count":0,"title":["Development of Anti\u2010Inflammatory Extracellular Vesicles by Surface Expression of Syndecan\u20104"],"prefix":"10.1002","volume":"15","author":[{"given":"Lijuan","family":"Yu","sequence":"first","affiliation":[{"name":"Krefting Research Centre, Department of Internal Medicine and Clinical Nutrition Institute of Medicine Sahlgrenska Academy University of Gothenburg  Gothenburg Sweden"},{"name":"Department of Laboratory Medicine, Guangdong Provincial Key Laboratory of Precision Medical Diagnostics, Guangdong Engineering and Technology Research Center for Rapid Diagnostic Biosensors, Guangdong 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