{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T22:30:20Z","timestamp":1790721020663,"version":"4.1.0"},"reference-count":60,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2026,4,17]],"date-time":"2026-04-17T00:00:00Z","timestamp":1776384000000},"content-version":"vor","delay-in-days":16,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T00:00:00Z","timestamp":1775001600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Extracellular Vesicle"],"published-print":{"date-parts":[[2026,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Extracellular vesicles (EVs) are emerging as diagnostic and therapeutic agents, yet their nanoscale size limits quantitative single\u2010vesicle analysis of surface epitopes and molecular interactions. Flow\u2010Induced Dispersion Analysis (FIDA) is a microfluidic technique enabling real\u2010time measurement of hydrodynamic size and interaction kinetics using fluorescent ligands without requiring surface immobilization. Here, FIDA was applied to characterize EV\u2013antibody interactions using anti\u2010CD63 antibodies across three EV sources: EVs from genetically engineered HEK cells expressing CD63\u2010eGFP or CD63\u2010NeonGreen\u2010Fc domain (CD63\u2010NG\u2010Fc), and EVs from clonally expanded immortalized mesenchymal stromal cells (ciMSCs). FIDA\u2010measured EV diameter sizes ranged from 40 to 90\u00a0nm, compared to 70\u2013200\u00a0nm as determined by Nanoparticle Tracking Analysis (NTA), likely reflecting methodological differences or NTA's sensitivity to non\u2010EV particles. CD63\u2010binding EC\n                    <jats:sub>50\u00a0<\/jats:sub>\n                    values were 1.9 \u00d7 10\n                    <jats:sup>8<\/jats:sup>\n                    \u00a0particles\/mL for CD63\u2010eGFP EVs and 8.4 \u00d7 10\n                    <jats:sup>8<\/jats:sup>\n                    to 2.4 \u00d7 10\n                    <jats:sup>9<\/jats:sup>\n                    \u00a0particles\/mL for ciMSC EVs, indicating higher epitope abundance in engineered vesicles. Within a broader antibody titration, maximal antibody loading to the Fc\u2010receptor domain on EVs was observed at 0.5\u20135\u00a0nM, reaching 30%\u201340% for IgG1 and 15%\u201320% for Cetuximab. These results establish FIDA as a high\u2010resolution, label\u2010efficient tool for quantifying EV\u2010antibody interactions and epitope accessibility, supporting its integration into EV standardization and quality control workflows.\n                  <\/jats:p>","DOI":"10.1002\/jev2.70269","type":"journal-article","created":{"date-parts":[[2026,4,17]],"date-time":"2026-04-17T12:47:10Z","timestamp":1776430030000},"source":"Crossref","is-referenced-by-count":1,"title":["In\u2010Solution Characterization of Extracellular Vesicles: A New Approach to Evaluating Antibody Binding and Surface Interactions"],"prefix":"10.1002","volume":"15","author":[{"given":"Maria\u2010Anthi","family":"Kakavoulia","sequence":"first","affiliation":[{"name":"Biochemistry and Chemistry, Department of Veterinary Sciences Ludwig\u2010Maximilians\u2010Universit\u00e4t Munich  M\u00fcnchen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Spies","sequence":"additional","affiliation":[{"name":"School of Life Sciences University of Applied Sciences  Muttenz Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6926-9003","authenticated-orcid":false,"given":"Tanja J.","family":"Kutzner","sequence":"additional","affiliation":[{"name":"Institute for Transfusion Medicine, University Hospital Essen University of Duisburg\u2010Essen  Essen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8659-8610","authenticated-orcid":false,"given":"Tobias","family":"Tertel","sequence":"additional","affiliation":[{"name":"Institute for Transfusion Medicine, University Hospital Essen University of Duisburg\u2010Essen  Essen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sophie","family":"Franzmeier","sequence":"additional","affiliation":[{"name":"Section of Clinical and Comparative Neuropathology, Institute of Veterinary Pathology Ludwig\u2010Maximilians\u2010Universit\u00e4t Munich  Munich Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samir El","family":"Andaloussi","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Division of Biomolecular and Cellular Medicine Karolinska Institutet  Stockholm Sweden"},{"name":"Department of Cellular Therapy and Allogeneic Stem Cell Transplantation (CAST) Karolinska University Hospital  Huddinge Sweden"},{"name":"Karolinska ATMP Center Karolinska Institutet, ANA Futura  Huddinge Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bernd","family":"Giebel","sequence":"additional","affiliation":[{"name":"Institute for Transfusion Medicine, University Hospital Essen University of Duisburg\u2010Essen  Essen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Herbert","family":"Kaltner","sequence":"additional","affiliation":[{"name":"Biochemistry and Chemistry, Department of Veterinary Sciences Ludwig\u2010Maximilians\u2010Universit\u00e4t Munich  M\u00fcnchen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2328-9794","authenticated-orcid":false,"given":"Dominik","family":"Meinel","sequence":"additional","affiliation":[{"name":"School of Life Sciences University of Applied Sciences  Muttenz Switzerland"},{"name":"Institute for Infectious Diseases University of Bern  Bern Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9198-0857","authenticated-orcid":false,"given":"Andr\u00e9","family":"G\u00f6rgens","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Division of Biomolecular and Cellular Medicine Karolinska Institutet  Stockholm Sweden"},{"name":"Department of Cellular Therapy and Allogeneic Stem Cell Transplantation (CAST) Karolinska University Hospital  Huddinge Sweden"},{"name":"Karolinska ATMP Center Karolinska Institutet, ANA Futura  Huddinge Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0901-839X","authenticated-orcid":false,"given":"Anna\u2010Kristin","family":"Ludwig","sequence":"additional","affiliation":[{"name":"Biochemistry and Chemistry, Department of Veterinary Sciences Ludwig\u2010Maximilians\u2010Universit\u00e4t Munich  M\u00fcnchen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,4,17]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1080\/1354750X.2024.2319296"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-0716-4314-3_7"},{"key":"e_1_2_9_4_1","article-title":"Imaging Extracellular Vesicles: Current and Emerging Methods","volume":"7","author":"Chuo S. 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