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This study reports that bacterial extracellular vesicles (BEVs) secreted by a soil bacterium\u00a0\n                    <jats:italic>Chryseobacterium aquifrigidense<\/jats:italic>\n                    M24 exhibit potent anti\u2010DENV activity by triggering the structural disintegration of DENV particles prior to cellular entry in a dose\u2010dependent manner. Mechanistic investigations revealed that BEVs interact with the viral envelope, inducing premature membrane fusion. This process is characterized by reduced membrane fluidity and irreversible lipid rearrangement, leading to a significant increase in particle density, as shown by iodixanol gradient ultracentrifugation. The proposed \u2018fusion\u2010triggered structural disruption\u2019 is further supported by the induction of aberrant E protein oligomerization and morphological changes observed via transmission electron microscopy. This mechanism is specific to enveloped viruses, as BEVs showed no effect on non\u2010enveloped Enterovirus 71. Crucially, this BEV\u2010mediated inactivation extends to other enveloped viruses, including HCV, WNV and YFV, indicating broad\u2010spectrum potential. Our findings reveal a previously unexplored function of BEVs as virucidal agents, proposing a new \u2018virus\u2010destructor\u2019 strategy that contrasts with conventional fusion inhibitors and offering promising avenues for developing broad\u2010spectrum antiviral drugs.\n                  <\/jats:p>","DOI":"10.1002\/jev2.70302","type":"journal-article","created":{"date-parts":[[2026,6,24]],"date-time":"2026-06-24T04:26:53Z","timestamp":1782275213000},"source":"Crossref","is-referenced-by-count":0,"title":["Bacterial Extracellular Vesicles (BEVs) Derived from\n                    <i>Chryseobacterium<\/i>\n                    Inhibit Dengue Virus Infection by Disrupting Its Structural Integrity"],"prefix":"10.1002","volume":"15","author":[{"given":"Yaqi","family":"Gao","sequence":"first","affiliation":[{"name":"Department of Microbiology, Shanghai Key Laboratory of Medical Biodefense, Faculty of Naval Medicine Naval Medical University  Shanghai 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