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Here, we identify CD133 (Prominin\u20101), selectively expressed in basal\u2010like triple\u2010negative breast cancer (BL\u2010TNBC) cells, acts as a key organizer of EV production and cargo composition. CD133 localizes to plasma membrane protrusions and lipid rafts, where it maintains membrane architecture, promotes EV release and directs selective cargo loading, including the pro\u2010angiogenic factor CD105. Functionally, CD133\n                    <jats:sup>+<\/jats:sup>\n                    EVs potently induce endothelial tubulogenesis in a CD105\u2010dependent manner, independently of differences in EV association or endothelial proliferation. In vivo, these EVs preferentially accumulate in the lung and liver, highlighting organ\u2010specific communication. Mechanistically, CD133 maintains lipid raft integrity, facilitating selective CD105 incorporation into vesicles and establishing a spatially coordinated CD133\u2010CD105 axis. Together, our findings define a subtype\u2010specific EV population whose identity and angiogenic activity are dictated by membrane organization. By linking protrusion architecture to vesicle\u2010mediated signalling, this study highlights CD133\u2010enriched EVs as potential biomarkers and therapeutic targets in aggressive BL\u2010TNBC.\n                  <\/jats:p>","DOI":"10.1002\/jev2.70303","type":"journal-article","created":{"date-parts":[[2026,5,29]],"date-time":"2026-05-29T02:54:26Z","timestamp":1780023266000},"source":"Crossref","is-referenced-by-count":1,"title":["CD133 Shapes Extracellular Vesicle Cargo and Angiogenic Function in Basal\u2010Like Triple\u2010Negative Breast Cancer"],"prefix":"10.1002","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0009-0000-5534-7878","authenticated-orcid":false,"given":"Mireia","family":"Gomez\u2010Duro","sequence":"first","affiliation":[{"name":"Structure and Membrane Compartments Institut Curie, PSL Research University  Paris 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