{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,30]],"date-time":"2026-10-30T04:21:17Z","timestamp":1793334077125,"version":"4.5.0"},"reference-count":56,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2026,7,17]],"date-time":"2026-07-17T00:00:00Z","timestamp":1784246400000},"content-version":"vor","delay-in-days":16,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100004608","name":"Natural Science Foundation of Jiangsu Province","doi-asserted-by":"publisher","award":["BK20220173"],"award-info":[{"award-number":["BK20220173"]}],"id":[{"id":"10.13039\/501100004608","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2022YFC2702503"],"award-info":[{"award-number":["2022YFC2702503"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82502009"],"award-info":[{"award-number":["82502009"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82201811"],"award-info":[{"award-number":["82201811"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Extracellular Vesicle"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Extracellular vesicles (EVs) in the mammalian oviduct constitute a key maternal regulatory system that maintains redox balance during early embryogenesis, yet their molecular cargo and functional relevance in human embryos remain poorly defined. Here, we show that human Fallopian tube\u2010derived EVs (oEVs) are rapidly internalized by human preimplantation embryos and improve developmental quality in vitro, increasing high\u2010quality Day 3 embryo formation and blastocyst development. Label\u2010free proteomics identified 6505 oEV proteins, with metabolic, antioxidant and stress\u2010response pathways strongly enriched. Cross\u2010reference with four independent datasets revealed a conserved protein subset shared across secretory\u2010phase oviduct fluid, pluripotent stem cell\u2010derived EVs and in\u2010vivo\u2010developed embryos; among these, YWHAZ was prioritized for functional validation because of its abundance in oEVs, its presence across embryo\u2010related datasets, and its known involvement in stress\u2010response pathways. We found that\n                    <jats:italic>Ywhaz<\/jats:italic>\n                    \u2010deficient mouse embryos exhibit elevated oxidative and apoptotic stress, transcriptional signatures of impaired glutathione metabolism, and failure to survive to birth despite normal blastocyst morphology. Recombinant YWHAZ protein alone failed to enter intact embryos, whereas engineered YWHAZ\u2010loaded EVs were efficiently internalized and significantly reduced intracellular ROS and apoptosis, restoring redox status towards in vivo levels without compromising implantation or foetal growth. Taken together, these findings identify YWHAZ protein as a conserved vesicle\u2010delivered regulator of redox homeostasis and demonstrate that EV\u2010mediated molecular delivery can partially rescue the oxidative stress burden characteristic of in vitro embryo culture. This work provides mechanistic insight into the maternal redox support system of the oviduct and establishes a foundation for EV\u2010based engineering of next\u2010generation embryo culture strategies.\n                  <\/jats:p>","DOI":"10.1002\/jev2.70337","type":"journal-article","created":{"date-parts":[[2026,7,17]],"date-time":"2026-07-17T13:00:16Z","timestamp":1784293216000},"source":"Crossref","is-referenced-by-count":1,"title":["Extracellular Vesicles From Human Fallopian Tubes Enhance IVF Embryo Development and Contain Functional Proteins Including YWHAZ"],"prefix":"10.1002","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3244-0353","authenticated-orcid":false,"given":"Yuehan","family":"Li","sequence":"first","affiliation":[{"name":"Reproductive Medicine Center, Tongji Hospital, Tongji Medical College Huazhong University of Science and Technology  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenjing","family":"Xiong","sequence":"additional","affiliation":[{"name":"Laboratory of Animal Center Huazhong University of Science and Technology  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Limin","family":"Gao","sequence":"additional","affiliation":[{"name":"Department of Reproductive Center Xiangyang Central Hospital Affiliated Hospital of Hubei University of Arts and Science  Xiangyang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-1352-6159","authenticated-orcid":false,"given":"Mingwei","family":"Lv","sequence":"additional","affiliation":[{"name":"Reproductive Medicine Center, Tongji Hospital, Tongji Medical College Huazhong University of Science and Technology  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fei","family":"Li","sequence":"additional","affiliation":[{"name":"Reproductive Medicine Center, Tongji Hospital, Tongji Medical College Huazhong University of Science and Technology  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rui","family":"Long","sequence":"additional","affiliation":[{"name":"Reproductive Medicine Center, Tongji Hospital, Tongji Medical College Huazhong University of Science and Technology  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chang","family":"Liu","sequence":"additional","affiliation":[{"name":"Reproductive Medicine Center Sichuan University West China Second University Hospital  Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianbo","family":"Wei","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology the First Affiliated Hospital of Guangzhou Medical University  Guangzhou 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China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8956-1944","authenticated-orcid":false,"given":"Na","family":"Guo","sequence":"additional","affiliation":[{"name":"Reproductive Medicine Center, Tongji Hospital, Tongji Medical College Huazhong University of Science and Technology  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0367-7765","authenticated-orcid":false,"given":"Lei","family":"Jin","sequence":"additional","affiliation":[{"name":"Reproductive Medicine Center, Tongji Hospital, Tongji Medical College Huazhong University of Science and Technology  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6927-4339","authenticated-orcid":false,"given":"Cong","family":"Sui","sequence":"additional","affiliation":[{"name":"Reproductive Medicine Center, Tongji Hospital, Tongji Medical College Huazhong University of Science 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