{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T19:12:39Z","timestamp":1788549159381,"version":"build-2803163510"},"reference-count":46,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2026,8,9]],"date-time":"2026-08-09T00:00:00Z","timestamp":1786233600000},"content-version":"vor","delay-in-days":8,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100000780","name":"European Commission","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000780","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000781","name":"European Research Council","doi-asserted-by":"publisher","award":["NanoBioMade 865629"],"award-info":[{"award-number":["NanoBioMade 865629"]}],"id":[{"id":"10.13039\/501100000781","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Extracellular Vesicle"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Efficient production of iron\u2010engineered extracellular vesicles (EVs) is of interest for their use as delivery systems in biotherapy. In this study, we investigated the role of iron in EV biogenesis and evaluated a scalable fluidic strategy for their mass production using turbulence stimulation in bioreactors. Murine mesenchymal stem cells were loaded with iron via ferric quinate or iron oxide nanoparticles (IONPs) and subsequently subjected to turbulence stimulation or serum starvation to induce EV secretion. Using elemental spectroscopy and cryogenic electron microscopy, we found that prolonged incubation with ferric quinate combined with turbulence stimulation resulted in the highest EV yield\u201460,000 EVs per cell in 3\u00a0h\u2014and enabled efficient iron transfer from cells to EVs. In contrast, IONP treatment did not increase EV yield compared to control and showed high variability in iron transfer, as probed by EV magnetophoretic mobility. TEM and XEDS analyses confirmed ferritin\u2010bound iron encapsulation within EVs, whereas IONPs were poorly internalised. These results highlight the promising role of ionic iron in EV formation and content, demonstrating how iron source, concentration, and incubation duration influence both EV biogenesis and iron engineering.<\/jats:p>","DOI":"10.1002\/jev2.70346","type":"journal-article","created":{"date-parts":[[2026,8,10]],"date-time":"2026-08-10T02:11:42Z","timestamp":1786327902000},"source":"Crossref","is-referenced-by-count":0,"title":["Iron Salts, but Not Iron Oxide Nanoparticles, Drive High\u2010Yield Production of Iron\u2010Engineered Extracellular Vesicles"],"prefix":"10.1002","volume":"15","author":[{"given":"Elliot","family":"Thouvenot","sequence":"first","affiliation":[{"name":"Laboratoire Physique des Cellules et Cancer Institut Curie, CNRS, Universit\u00e9 PSL  Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aurore","family":"Van de Walle","sequence":"additional","affiliation":[{"name":"Laboratoire Physique des Cellules et Cancer Institut Curie, CNRS, Universit\u00e9 PSL  Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean\u2010Michel","family":"Guigner","sequence":"additional","affiliation":[{"name":"Institut de Min\u00e9ralogie, de Physique des Mat\u00e9riaux et de Cosmochimie IMPMC MNHN CNRS Sorbonne Universit\u00e9  Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ali","family":"Abou\u2010Hassan","sequence":"additional","affiliation":[{"name":"Physicochimie des \u00c9lectrolytes et Nanosyst\u00e8mes InterfaciauX (PHENIX) CNRS Sorbonne Universit\u00e9  Paris France"},{"name":"Institut Universitaire de France (IUF)  Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1511-8576","authenticated-orcid":false,"given":"Lorena","family":"Martin\u2010Jaular","sequence":"additional","affiliation":[{"name":"INSERM U932 Institut Curie Research Center PSL Research University  Paris France"},{"name":"CurieCoreTech ExtracellularVesicles Institut Curie Research Center  Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amanda K. A.","family":"Silva","sequence":"additional","affiliation":[{"name":"Laboratoire Mati\u00e8re et Syst\u00e8mes Complexes, MSC, CNRS UMR7057 Universit\u00e9 Paris Cit\u00e9  Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7024-9627","authenticated-orcid":false,"given":"Claire","family":"Wilhelm","sequence":"additional","affiliation":[{"name":"Laboratoire Physique des Cellules et Cancer Institut Curie, CNRS, Universit\u00e9 PSL  Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,8,9]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1039\/c3nr01541f"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.matbio.2017.05.001"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pharmthera.2020.107683"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsami.8b18270"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1039\/D0NR07349K"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0033115"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.3390\/nano10081548"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41420\u2010023\u201001490\u20102"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/jev2.12144"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms241713247"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/jev2.12111"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/adfm.201803660"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsabm.1c01200"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.203.4387.1355"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010023\u201040258\u20101"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41580\u2010023\u201000648\u20101"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.addr.2021.113843"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/s43586\u2010023\u201000240\u2010z"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrm.25376"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/jev2.12085"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/jev2.70110"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2020.119942"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.scr.2009.12.003"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jconrel.2020.07.032"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/s44222\u2010024\u201000255\u20105"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41556\u2010018\u20100250\u20109"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.3390\/pharmaceutics12070676"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsnano.0c09938"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.3390\/ph18030325"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1021\/nn400269x"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.nano.2014.11.009"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11095\u2010012\u20100680\u20101"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.bioconjchem.8b00732"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1080\/17425247.2022.2047020"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0704879104"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2017.09.021"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.4236\/jbnb.2011.225060"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood\u20102017\u201002\u2010768580"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2020.08.001"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms22094591"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.1002\/jev2.70178"},{"key":"e_1_2_10_43_1","doi-asserted-by":"publisher","DOI":"10.1002\/jev2.12404"},{"key":"e_1_2_10_44_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.2021010995"},{"key":"e_1_2_10_45_1","doi-asserted-by":"publisher","DOI":"10.3402\/jev.v4.27066"},{"key":"e_1_2_10_46_1","doi-asserted-by":"publisher","DOI":"10.1002\/stem.1771"},{"key":"e_1_2_10_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2021.07.027"}],"container-title":["Journal of Extracellular Vesicles"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/isevjournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jev2.70346","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/isevjournals.onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jev2.70346","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/isevjournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jev2.70346","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T18:36:29Z","timestamp":1788546989000},"score":1,"resource":{"primary":{"URL":"https:\/\/isevjournals.onlinelibrary.wiley.com\/doi\/10.1002\/jev2.70346"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8]]},"references-count":46,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2026,8]]}},"alternative-id":["10.1002\/jev2.70346"],"URL":"https:\/\/doi.org\/10.1002\/jev2.70346","archive":["Portico"],"relation":{},"ISSN":["2001-3078","2001-3078"],"issn-type":[{"value":"2001-3078","type":"print"},{"value":"2001-3078","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8]]},"article-number":"e70346"}}