{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T19:12:40Z","timestamp":1788549160577,"version":"build-2803163510"},"reference-count":55,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2026,8,9]],"date-time":"2026-08-09T00:00:00Z","timestamp":1786233600000},"content-version":"vor","delay-in-days":8,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["CRC1700"],"award-info":[{"award-number":["CRC1700"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["GO 2528\/10\u20101"],"award-info":[{"award-number":["GO 2528\/10\u20101"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["GRK 2873"],"award-info":[{"award-number":["GRK 2873"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Extracellular Vesicle"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Plasma proteins are increasingly recognized as key regulators of extracellular vesicle (EV) behaviour in circulation. However, von Willebrand factor (vWF)\u2014the largest multimeric glycoprotein in blood and the only blood protein activated by shear\u2014has received little attention in this context. Under elevated shear stress, vWF transitions from a compact globular form to an extended adhesive conformation. Although elevated plasma vWF levels are associated with hypercoagulation in conditions such as malaria, COVID\u201019, and cancer, its interactions with EVs or cells under physiological flow have not been systematically investigated. Here, we show that shear\u2010activated vWF functions as a size\u2010selective molecular filter that preferentially captures objects smaller than \u223c4 \u00b5m, including platelets and tumour\u2010derived EVs, while excluding larger cells. Although intact tumour cells do not directly bind vWF under physiological flow, the coordinated binding of EVs and platelets to extended vWF promotes platelet aggregation, which subsequently traps circulating tumour cells and fosters metastatic dissemination. Our findings reveal a previously unrecognized role of vWF as a shear\u2010dependent EV\u2010binding protein that brings EVs and platelets together to enhance coagulation and metastasis. These EV\u2013vWF\u2013platelet aggregates may represent promising biomarkers or therapeutic targets for the prevention of hypercoagulation in cancer and other thrombo\u2010inflammatory diseases.<\/jats:p>","DOI":"10.1002\/jev2.70349","type":"journal-article","created":{"date-parts":[[2026,8,10]],"date-time":"2026-08-10T02:13:19Z","timestamp":1786327999000},"source":"Crossref","is-referenced-by-count":0,"title":["Shear\u2010Activated von Willebrand Factor Captures Extracellular Vesicles to Promote Platelet Activation and Metastasis"],"prefix":"10.1002","volume":"15","author":[{"given":"Yuanyuan","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Dermatology University Medical Center Hamburg\u2010Eppendorf  Hamburg Germany"},{"name":"Mildred Scheel Cancer Career Center HaTriCS4 University Medical Center Hamburg\u2010Eppendorf  Hamburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaobo","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Dermatology University Medical Center Hamburg\u2010Eppendorf  Hamburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pascal","family":"Nakielski","sequence":"additional","affiliation":[{"name":"Center for Hybrid Nanostructures (CHyN) Universit\u00e4t Hamburg  Hamburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katrin","family":"Nekipelov","sequence":"additional","affiliation":[{"name":"Pharmaceutical Institute University of Bonn  Bonn Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amanda","family":"Salviano\u2010Silva","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery University Medical Center Hamburg\u2010Eppendorf  Hamburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alper","family":"Topuz","sequence":"additional","affiliation":[{"name":"Institute for Advanced Simulation (IAS\u20102) Forschungszentrum J\u00fclich  J\u00fclich Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexander T.","family":"Bauer","sequence":"additional","affiliation":[{"name":"Department of Dermatology University Medical Center Hamburg\u2010Eppendorf  Hamburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julian","family":"K\u00f6tt","sequence":"additional","affiliation":[{"name":"Department of Dermatology University Medical Center Hamburg\u2010Eppendorf  Hamburg Germany"},{"name":"Fleur Hiege Center for Skin Cancer Research University Medical Center Hamburg\u2010Eppendorf  Hamburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jannis","family":"Akrivakis","sequence":"additional","affiliation":[{"name":"Department of Dermatology University Medical Center Hamburg\u2010Eppendorf  Hamburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Santra","family":"Brenna","sequence":"additional","affiliation":[{"name":"Neurology Department Experimental Research in Stroke and Inflammation (ERSI) University Medical Center Hamburg\u2010Eppendorf  Hamburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tomasz","family":"Downar","sequence":"additional","affiliation":[{"name":"Experimental Dermatology Medical Faculty Mannheim Heidelberg University  Mannheim Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Neus","family":"Feliu","sequence":"additional","affiliation":[{"name":"Center for Hybrid Nanostructures (CHyN) Universit\u00e4t Hamburg  Hamburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gerd","family":"Bendas","sequence":"additional","affiliation":[{"name":"Pharmaceutical Institute University of Bonn  Bonn Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2255-8393","authenticated-orcid":false,"given":"Berta","family":"Puig","sequence":"additional","affiliation":[{"name":"Neurology Department Experimental Research in Stroke and Inflammation (ERSI) University Medical Center Hamburg\u2010Eppendorf  Hamburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefan W.","family":"Schneider","sequence":"additional","affiliation":[{"name":"Department of Dermatology University Medical Center Hamburg\u2010Eppendorf  Hamburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dmitry A.","family":"Fedosov","sequence":"additional","affiliation":[{"name":"Institute for Advanced Simulation (IAS\u20102) Forschungszentrum J\u00fclich  J\u00fclich Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3788-6429","authenticated-orcid":false,"given":"Christian","family":"Gorzelanny","sequence":"additional","affiliation":[{"name":"Department of Dermatology University Medical Center Hamburg\u2010Eppendorf  Hamburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,8,9]]},"reference":[{"key":"e_1_2_14_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms22158163"},{"key":"e_1_2_14_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/bjh.18616"},{"key":"e_1_2_14_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bpj.2010.06.002"},{"key":"e_1_2_14_5_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood\u20102014\u201008\u2010595686"},{"key":"e_1_2_14_6_1","doi-asserted-by":"publisher","DOI":"10.1080\/20013078.2020.1809065"},{"key":"e_1_2_14_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12032\u2010023\u201001950\u2010w"},{"key":"e_1_2_14_8_1","doi-asserted-by":"publisher","DOI":"10.1039\/C3NR05107B"},{"key":"e_1_2_14_9_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood\u20102004\u201002\u20100434"},{"key":"e_1_2_14_10_1","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2017.00261"},{"key":"e_1_2_14_11_1","doi-asserted-by":"publisher","DOI":"10.1039\/c8nr04042g"},{"key":"e_1_2_14_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/jth.15794"},{"key":"e_1_2_14_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.nano.2011.04.003"},{"key":"e_1_2_14_14_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0069774"},{"key":"e_1_2_14_15_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.2020005710"},{"key":"e_1_2_14_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010017\u201000230\u20102"},{"key":"e_1_2_14_17_1","doi-asserted-by":"publisher","DOI":"10.1160\/th07\u201003\u20100222"},{"key":"e_1_2_14_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010024\u201047516\u2010w"},{"key":"e_1_2_14_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/s43018\u2010021\u201000272\u2010y"},{"key":"e_1_2_14_20_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood\u20102013\u201004\u2010460139"},{"key":"e_1_2_14_21_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.11832"},{"key":"e_1_2_14_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/pgr2.70017"},{"key":"e_1_2_14_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccell.2018.03.002"},{"key":"e_1_2_14_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/imr.13106"},{"key":"e_1_2_14_25_1","doi-asserted-by":"publisher","DOI":"10.1160\/th13\u201009\u20100800"},{"key":"e_1_2_14_26_1","doi-asserted-by":"publisher","DOI":"10.1182\/bloodadvances.2017013995"},{"key":"e_1_2_14_27_1","doi-asserted-by":"publisher","DOI":"10.1292\/jvms.19\u20100400"},{"key":"e_1_2_14_28_1","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.adp1461"},{"key":"e_1_2_14_29_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2024.1367373"},{"key":"e_1_2_14_30_1","doi-asserted-by":"publisher","DOI":"10.1160\/th11\u201003\u20100143"},{"key":"e_1_2_14_31_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2019.01453"},{"key":"e_1_2_14_32_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2122716119"},{"key":"e_1_2_14_33_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.2025029974"},{"key":"e_1_2_14_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2025.01.025"},{"key":"e_1_2_14_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jtha.2023.03.027"},{"key":"e_1_2_14_36_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells10092351"},{"key":"e_1_2_14_37_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm.1955"},{"key":"e_1_2_14_38_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010018\u201028961\u20102"},{"key":"e_1_2_14_39_1","doi-asserted-by":"publisher","DOI":"10.1002\/rth2.12641"},{"key":"e_1_2_14_40_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood\u20102010\u201003\u2010276311"},{"key":"e_1_2_14_41_1","doi-asserted-by":"publisher","DOI":"10.1039\/C3MH00106G"},{"key":"e_1_2_14_42_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010017\u201014346\u20104"},{"key":"e_1_2_14_43_1","doi-asserted-by":"publisher","DOI":"10.1160\/th11\u201010\u20100719"},{"key":"e_1_2_14_44_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010021\u201002254\u20107"},{"key":"e_1_2_14_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.rpth.2023.100124"},{"key":"e_1_2_14_46_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12860\u2010020\u201000309\u20107"},{"key":"e_1_2_14_47_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0608422104"},{"key":"e_1_2_14_48_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.94.1.316"},{"key":"e_1_2_14_49_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010020\u201079882\u2010y"},{"key":"e_1_2_14_50_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092\u20108674(91)90648\u2010i"},{"key":"e_1_2_14_51_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2022.1045624"},{"key":"e_1_2_14_52_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.matbio.2022.06.002"},{"key":"e_1_2_14_53_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers15113025"},{"key":"e_1_2_14_54_1","doi-asserted-by":"publisher","DOI":"10.1002\/jev2.12404"},{"key":"e_1_2_14_55_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2019.04.003"},{"key":"e_1_2_14_56_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2020.610696"}],"container-title":["Journal of Extracellular Vesicles"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/isevjournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jev2.70349","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/isevjournals.onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jev2.70349","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/isevjournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jev2.70349","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T18:36:34Z","timestamp":1788546994000},"score":1,"resource":{"primary":{"URL":"https:\/\/isevjournals.onlinelibrary.wiley.com\/doi\/10.1002\/jev2.70349"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8]]},"references-count":55,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2026,8]]}},"alternative-id":["10.1002\/jev2.70349"],"URL":"https:\/\/doi.org\/10.1002\/jev2.70349","archive":["Portico"],"relation":{},"ISSN":["2001-3078","2001-3078"],"issn-type":[{"value":"2001-3078","type":"print"},{"value":"2001-3078","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8]]},"article-number":"e70349"}}