{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T19:12:39Z","timestamp":1788549159058,"version":"build-2803163510"},"reference-count":34,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T00:00:00Z","timestamp":1785715200000},"content-version":"vor","delay-in-days":2,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["2022R1A2C2006898"],"award-info":[{"award-number":["2022R1A2C2006898"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["2020R1A4A2002691"],"award-info":[{"award-number":["2020R1A4A2002691"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["RS\u20102023\u201000222188"],"award-info":[{"award-number":["RS\u20102023\u201000222188"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003710","name":"Korea Health Industry Development Institute","doi-asserted-by":"publisher","award":["RS\u20102024\u201000438983"],"award-info":[{"award-number":["RS\u20102024\u201000438983"]}],"id":[{"id":"10.13039\/501100003710","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Extracellular Vesicle"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Metabolic dysfunction\u2010associated steatotic liver disease (MASLD) and metabolic dysfunction\u2010associated steatohepatitis are leading causes of chronic liver disease, yet the contribution of neutrophils to early hepatocellular lipid accumulation remains poorly understood. Here, we investigated the role of neutrophils in hepatic lipid deposition during early MASLD development. Neutrophils acquired extracellular fatty acids (FAs) via FATP2 and CD36 and stored them as triglycerides (TGs). These lipid\u2010laden neutrophils (LNs) did not utilize FAs for energy production or lipid mediator synthesis but instead transferred lipid cargo to hepatocytes through extracellular vesicles (EVs). Neutrophil\u2010derived EVs were enriched with TGs and lipid metabolism\u2010regulating microRNAs, augmenting TG accumulation in hepatocytes. Peripheral neutrophils isolated from high\u2010fat diet\u2010fed mice exhibited a lipid\u2010laden phenotype, and adoptive transfer of LNs and EVs derived from LNs increased hepatic fat accumulation in recipient mice. In patients with MASLD, circulating neutrophils showed lipid droplet accumulation with increased TGs and enrichment of lipid\u2010associated miRNAs while plasma EVs were also enriched with TGs and lipid\u2010associated miRNAs. Single\u2010cell RNA sequencing of peripheral immune cells identified a neutrophil subpopulation characterized by enhanced lipid\u2010handling and EV\u2010related gene expression. These findings identify neutrophil\u2010mediated lipid transfer via EVs as a mechanistic link between innate immune activation and hepatic lipid accumulation during early MASLD.<\/jats:p>","DOI":"10.1002\/jev2.70350","type":"journal-article","created":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T12:52:11Z","timestamp":1785761531000},"source":"Crossref","is-referenced-by-count":0,"title":["Neutrophils Promote Metabolic Dysfunction\u2010Associated Steatotic Liver Disease Through Extracellular Vesicle\u2010mediated Lipid Transfer"],"prefix":"10.1002","volume":"15","author":[{"given":"Sanjeeb","family":"Shrestha","sequence":"first","affiliation":[{"name":"Department of Physiology School of Medicine Kyungpook National University  Daegu South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Soo\u2010Jung","family":"Jung","sequence":"additional","affiliation":[{"name":"Department of Physiology School of Medicine Kyungpook National University  Daegu South Korea"},{"name":"Department of Anatomy College of Korean Medicine Daejeon University  Daejeon South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seong\u2010Min","family":"Lim","sequence":"additional","affiliation":[{"name":"Department of Physiology School of Medicine Kyungpook National University  Daegu South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu\u2010Bin","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Physiology School of Medicine Kyungpook National University  Daegu South Korea"},{"name":"Safety &amp; Health Team Kyonggi University  Suwon Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hyeung\u2010Wook","family":"Shin","sequence":"additional","affiliation":[{"name":"Department of Physiology School of Medicine Kyungpook National University  Daegu South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hoyul","family":"Lee","sequence":"additional","affiliation":[{"name":"Research Institute of Aging and Metabolism Kyungpook National University  Daegu South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hee\u2010Kyung","family":"Jin","sequence":"additional","affiliation":[{"name":"Department of Laboratory Animal Medicine College of Veterinary Medicine Kyungpook National University  Daegu South Korea"},{"name":"KNU Alzheimer's disease Research Institute Kyungpook National University  Daegu South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jae\u2010Sung","family":"Bae","sequence":"additional","affiliation":[{"name":"Department of Physiology School of Medicine Kyungpook National University  Daegu South Korea"},{"name":"Department of Laboratory Animal Medicine College of Veterinary Medicine Kyungpook National University  Daegu South Korea"},{"name":"KNU Alzheimer's disease Research Institute Kyungpook National University  Daegu South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jae\u2010Han","family":"Jeon","sequence":"additional","affiliation":[{"name":"Research Institute of Aging and Metabolism Kyungpook National University  Daegu South Korea"},{"name":"Department of Internal Medicine School of Medicine Kyungpook National University Kyungpook National University Chilgok Hospital  Daegu South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lai Guan","family":"Ng","sequence":"additional","affiliation":[{"name":"School of Medicine Westlake University Hangzhou China"},{"name":"Shanghai Immune Therapy Institute Shanghai Jiao Tong University School of Medicine Affiliated to Renji Hospital  Shanghai China"},{"name":"Department of Microbiology and Immunology National University of Singapore (NUS) Singapore Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7745-9205","authenticated-orcid":false,"given":"Chang\u2010Won","family":"Hong","sequence":"additional","affiliation":[{"name":"Department of Physiology School of Medicine Kyungpook National University  Daegu South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,8,3]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1080\/2162402X.2017.1344804"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1478-3231.2011.02639.x"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1136\/gutjnl-2016-313654"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1500500"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mce.2010.07.005"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.3390\/biomedicines13092211"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra1503519"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI23621"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41591-018-0104-9"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.26937"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-023-37674-8"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI141513"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.4110\/in.2018.18.e43"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.31031"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.3389\/fendo.2021.751802"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2022.120422"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41590-020-0783-5"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aaa4352"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-0716-0802-9_9"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri.2016.70"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12020-017-1323-4"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.00521.2005"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.1097\/HEP.0000000000000520"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40001-025-03215-w"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2021.625472"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells11121883"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncb1596"},{"key":"e_1_2_11_29_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.29914"},{"key":"e_1_2_11_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2021.07.032"},{"key":"e_1_2_11_31_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10753-021-01442-x"},{"key":"e_1_2_11_32_1","doi-asserted-by":"publisher","DOI":"10.1002\/jev2.12404"},{"issue":"3","key":"e_1_2_11_33_1","first-page":"278","article-title":"Neutrophil\u2010Lymphocyte Ratio (NLR) Could Be Better Predictor Than C\u2010reactive Protein (CRP) for Liver Fibrosis in Non\u2010alcoholic Steatohepatitis(NASH)","volume":"45","author":"Yilmaz H.","year":"2015","journal-title":"Annals of Clinical and Laboratory Science"},{"key":"e_1_2_11_34_1","doi-asserted-by":"publisher","DOI":"10.7150\/thno.51756"},{"key":"e_1_2_11_35_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12986-020-0434-8"}],"container-title":["Journal of Extracellular Vesicles"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/isevjournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jev2.70350","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/isevjournals.onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jev2.70350","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/isevjournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jev2.70350","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T18:36:18Z","timestamp":1788546978000},"score":1,"resource":{"primary":{"URL":"https:\/\/isevjournals.onlinelibrary.wiley.com\/doi\/10.1002\/jev2.70350"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8]]},"references-count":34,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2026,8]]}},"alternative-id":["10.1002\/jev2.70350"],"URL":"https:\/\/doi.org\/10.1002\/jev2.70350","archive":["Portico"],"relation":{},"ISSN":["2001-3078","2001-3078"],"issn-type":[{"value":"2001-3078","type":"print"},{"value":"2001-3078","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8]]},"article-number":"e70350"}}