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Here, we characterise the EV protein and miRNA cargo of\u00a0T. suis\u00a0to identify potential roles in host\u2013parasite communication. In contrast to EVs from\u00a0clade III\/V helminth species characterised to date, we show that\u00a0T. suis\u00a0EVs did not suppress LPS\u2010induced TNF production by human macrophages under the conditions tested, in contrast to non\u2010vesicular ESP fractions which did. By generating the first extracellular miRNA complement for\u00a0T. suis\u00a0and quantifying EV\u2010associated versus free miRNAs across larval and adult stages, we identify selective enrichment of two miRNAs in adult EVs with homology to human miR\u201022\u20103p and miR\u201029\u20103p, canonical anti\u2010fibrotic and tumour\u2010suppressor miRNAs. Functional assays in human colon cancer epithelial cells reveal that these helminth\u2010derived orthologues downregulate conserved oncogenic targets, indicating evolutionary conservation of their regulatory capacity. Our findings uncover a previously unrecognised axis of whipworm biology in which selective EV\u2010mediated export of anti\u2010fibrotic miRNAs has the potential to modulate host tissue remodeling independently of classical immunomodulation, with implications for understanding helminth\u2010driven epithelial homeostasis and cancer risk.\n                  <\/jats:p>","DOI":"10.1002\/jev2.70351","type":"journal-article","created":{"date-parts":[[2026,8,29]],"date-time":"2026-08-29T18:34:06Z","timestamp":1788028446000},"source":"Crossref","is-referenced-by-count":0,"title":["Selective Packaging of miR\u201022 and miR\u201029 Orthologues into Extracellular Vesicles of the Porcine Whipworm\n                    <i>Trichuris Suis<\/i>"],"prefix":"10.1002","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0009-0000-8231-1707","authenticated-orcid":false,"given":"Bradley","family":"Whitehead","sequence":"first","affiliation":[{"name":"Department of Clinical Medicine Faculty of Health Aarhus University  Aarhus Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maibritt","family":"Mardahl","sequence":"additional","affiliation":[{"name":"Department of Clinical Medicine Faculty of Health Aarhus University  Aarhus Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eline P.","family":"Hansen","sequence":"additional","affiliation":[{"name":"Department of Veterinary and Animal Sciences Faculty of Health and Medical Sciences University of Copenhagen  Copenhagen Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bastian","family":"Fromm","sequence":"additional","affiliation":[{"name":"The Arctic University Museum of Norway UiT \u2013 The Arctic University of Norway  Troms\u00f8 Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7710-4391","authenticated-orcid":false,"given":"Anne","family":"Borup","sequence":"additional","affiliation":[{"name":"Department of Clinical Medicine Faculty of Health Aarhus University  Aarhus Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0192-1413","authenticated-orcid":false,"given":"Anders Toftegaard","family":"Boysen","sequence":"additional","affiliation":[{"name":"Department of Clinical Medicine Faculty of Health Aarhus University  Aarhus Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jasmine C. L.","family":"Atay","sequence":"additional","affiliation":[{"name":"Department of Clinical Medicine Faculty of Health Aarhus University  Aarhus Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rikke","family":"N\u00f8rregaard","sequence":"additional","affiliation":[{"name":"Department of Clinical Medicine Faculty of Health Aarhus University  Aarhus Denmark"},{"name":"Department of Renal Medicine Aarhus University Hospital  Aarhus Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Allan","family":"Stensballe","sequence":"additional","affiliation":[{"name":"Department of Health Science and Technology Aalborg University  Aalborg Denmark"},{"name":"Clinical Cancer Research Center Aalborg University Hospital  Aalborg Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sidsel D.","family":"Andersen","sequence":"additional","affiliation":[{"name":"Department of Clinical Medicine Faculty of Health Aarhus University  Aarhus 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